Vascular Endothelial Growth Factor (VEGF) Expression in Human Coronary Atherosclerotic Lesions

Inoue, Mayumi; Itoh, Hiroshi; Ueda, Makiko; Naruko, Takahiko; Kojima, Akiko; Komatsu, Ryushi; Doi, Kentaro; Ogawa, Yoshihiro; Tamura, Naohisa; Takaya, Kazuhiko; Igaki, Toshio; Yamashita, Jun; Chun, Tae Hwa; Masatsugu, Ken; Becker, Anton E.; Nakao, Kazuwa

doi:10.1161/01.cir.98.20.2108

Cited by 410 publications

(136 citation statements)

References 24 publications

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“…VEGF can induce the migration and proliferation of endothelial cells and increase vascular permeability (Inoue et al, 1998). These events are consistent with the capacity to promote angiogenesis, which has a central role in cicatrization.…”

Section: Resultssupporting

confidence: 60%

Wound-healing evaluation of ointment from Stryphnodendron adstringens (barbatimão) in rat skin

Hernandes

Pereira

Palazzo

et al. 2010

Braz. J. Pharm. Sci.

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This study evaluated the cicatrizant effect of an ointment containing 1% of the ethyl-acetate fraction extracted from the stem bark of "barbatimão" (Stryphnodendron adstringens), in wounds made in the skin of rats, after 4, 7 and 10 days of treatment. Control wounds were treated with a base ointment without extract. The proliferation of keratinocytes in the area of reepithelialization was evaluated by counting the number of epithelial cells that were blocked in metaphase by vincristine sulfate. The length of the reepithelialized margin and the contraction of the wound were measured. Topical application of the "barbatimão" ointment stimulated proliferation of the keratinocytes, but had no effect on the length of the epithelium or on the contraction of the wounds. Uniterms:Stryphnodendron adstringens/pharmacognosy. Stryphnodendron adstringens/ cicatrizant effect. "Barbatimão"/pharmacognosy. Wound/healing/experimental study. Reepithelialization.Neste estudo, avaliou-se a atividade cicatrizante de uma pomada contendo uma fração acetato de etila 1% obtida de cascas de "barbatimão" (Stryphnodendron adstringens) em feridas excisionais na pele de ratos após 4, 7 e 10 dias de tratamento. Feridas controle foram tratadas com pomada base, sem extrato. A proliferação dos queratinócitos na área reepitelizada foi avaliada através da contagem do número de queratinócitos bloqueados em metáfase, pelo sulfato de vincristina. O comprimento da margem reepitelizada e a contração das feridas foram mensurados. As feridas tratadas com barbatimão apresentaram um maior número de mitoses do que aquelas tratadas com a pomada base, em todos os tempos avaliados. A aplicação tópica da pomada de "barbatimão" estimulou a proliferação epitelial contudo não teve efeito sobre a migração dos queratinócitos ou sobre a contração das feridas. Unitermos:Stryphnodendron adstringens/farmacognosia. Stryphnodendron adstringens/efeito cicatrizante. "Barbatimão"/farmacognosia. Feridas/cicatrização/estudo experimental. Reepitelização. INTRODUCTIONIn cutaneous lesions, tannins bind to the proteins of the injured tissues, precipitating them (Fernandez et al., 2002) and creating a protective layer (Neto et al., 1996). This layer isolates the wound site from the environment, reducing the permeability and exudation of the wound (Brown, Dattner, 1998;Bedi, Shenefelt, 2002) and promoting tissue repair. Tannins also exhibit vasoconstrictor and anti-inflammatory properties (Mota et al., 1985;Kapu et al., 2001) and stimulate the growth of epidermis, aiding reepithelialization (Palermo et al., 2002;. Reepithelialization involves the proliferation and migration of cells from the edges of the wound, and is regulated by mechanisms involving genes, growth factors, integrins, extracellular matrices (ECM) and metalloproteinases (MMPs) (Santoro, Gaudino, 2005).Stryphnodendron Martius, Leguminosae, popularly known as "barbatimão", is a Brazilian savannah tree. Extracts of the stem bark from species of Stryphnodendron have several medically useful properties, such as antiinflamma...

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Section: Resultssupporting

confidence: 60%

Wound-healing evaluation of ointment from Stryphnodendron adstringens (barbatimão) in rat skin

Hernandes

Pereira

Palazzo

et al. 2010

Braz. J. Pharm. Sci.

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“…Moreover, VEGF has been found to be upregulated in human coronary atherosclerotic lesions but not in normal coronary segments [9]. Although the mechanism by which VEGF induces plaque formation has not been established, it has been suggested that new vessel formation causes plaque progression [8].…”

Section: Introductionmentioning

confidence: 99%

Vitamins C and E downregulate vascular VEGF and VEGFR-2 expression in apolipoprotein-E-deficient mice

et al. 2003

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Anti-angiogenic therapy reduces both plaque growth and intimal neovascularization in apolipoprotein-E-deficient mice (apoE−/−). Vascular endothelial growth factor (VEGF) has been suggested as playing a role in the development of atherosclerosis. We examined the hypothesis that VEGF and VEGF receptor-2 (VEGFR-2) expression is upregulated in apoE−/− and, since it could be driven by oxidative stress, tested whether dietary supplementation with vitamins C and E could downregulate it. Two-month-old apoE−/− received vitamin C combined with ␣-or ␤-tocopherol for 4 weeks. Aortic VEGF and VEGFR-2 expression were measured by RT-qPCR and western blot.ApoE−/− showed significantly higher expression of aortic VEGF and VEGFR-2 mRNA (P < 0.001) and protein (P < 0.001) than wild-type mice, as well as increased plasma VEGF (P < 0.001). Vitamin C and ␣-tocopherol significantly reduced aortic VEGF and VEGFR-2 expression in apoE−/− (P < 0.001), circulating VEGF (P < 0.01) and plasma lipid peroxidation (P < 0.01). apoE−/− receiving vitamin C and ␤-tocopherol showed diminished lipid peroxidation and VEGFR-2, but only partial reduction of VEGF expression.These data demonstrate that augmented VEGF and VEGFR-2 expression in apoE−/− vasculature can be downregulated by vitamins C and E, at least partially through oxidative stress reduction. This novel mechanism could contribute to explaining the beneficial effects of antioxidant vitamins in experimental atherosclerosis.

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“…However, it remains uncertain whether VEGF enhances or prevents IVD degeneration. Increased VEGF expression has been associated with the progress of atherosclerosis through neovascularization and inflammation in atheromatous plaques (Inoue et al, 1998;Celletti et al, 2001). Some studies have shown that expression of VEGF and nerve growth factor are significantly higher in degenerative disc with no herniation compared with herniated disc, and angiogenesis is related to the presence of nerve fiber ingrowth into degenerative IVD and the appearance of discogenic low back pain (Freemont et al, 1997(Freemont et al, , 2002.…”

Section: Discussionmentioning

confidence: 99%

“…PCR-RFLP assay was performed to analyze these 3 SNPs. The primers and PCR conditions for the determination of eNOS SNP have been previously described (Inoue et al, 1998).…”

Section: Genotypingmentioning

confidence: 99%

Association between VEGF and eNOS gene polymorphisms and lumbar disc degeneration in a young Korean population

Han¹,

Ropper²,

Teng³

et al. 2013

Genet. Mol. Res.

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Vascular Endothelial Growth Factor (VEGF) Expression in Human Coronary Atherosclerotic Lesions

Cited by 410 publications

References 24 publications

Wound-healing evaluation of ointment from Stryphnodendron adstringens (barbatimão) in rat skin

Wound-healing evaluation of ointment from Stryphnodendron adstringens (barbatimão) in rat skin

Vitamins C and E downregulate vascular VEGF and VEGFR-2 expression in apolipoprotein-E-deficient mice

Association between VEGF and eNOS gene polymorphisms and lumbar disc degeneration in a young Korean population

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