Vascular imaging in small rodents using micro-CT

Replacing Vascular Corrosion Casting by In Vivo Micro-CT Imaging for Building 3D Cardiovascular Models in Mice

“…Schambach et al [19] have already used both a blood pool contrast agent [21] showed that the morphology of 3D models of the murine aortic arch (obtained using vascular corrosion casting) is very similar to the one of the human aortic arch (obtained in vivo). An alternative method to obtain 3D models of the murine arterial geometry in vivo is MRI imaging.…”

In Vivo X-Ray Digital Subtraction and CT Angiography of the Murine Cerebrovasculature Using an Intra-Arterial Route of Contrast Injection

“…While other groups performed in vivo x-ray DSA of liver, 19 thoracic, and cerebral vessels in rats, 11,17,18,21 and of the renal vasculature in mice, 17 no studies described x-ray DSA for imaging of the cerebrovasculature in live mice. Our findings, however, demonstrate that this is highly achievable.…”

“…11,15,17 Because most institutions do not have easy access to a synchrotron, smaller, less expensive, and more accessible methods are preferred. For example, some researchers have implemented x-ray-based DSA of the thoracic and liver vasculature in mice.…”

Synchrotron Radiation and Nanotechnology for Stem Cell Research

“…At tissue level of organization, microscopy techniques attempting to visualize the tissue rebuilding process, such as light, fluorescence, scanning and transmission electron microscopy are limited to two-dimensional (2D) local information or, otherwise, require laborious three-dimensional (3D) reconstruction of serial sections. In-vivo imaging methods, including MRI, PET and micro-CT, have the potential to play a major role in the setting attempting to allow quantification of the rebuilding process, including longitudinal cell tracking (Badea et al, 2006;De Vries et al, 2005;Dhodapkar et al, 2001;Schambach et al, 2010). On the other hand, current radiologic 3D-methods, possess intrinsic limitations to identify the localization and fate of the injected cells in both clinical (Frangioni & Hajjar , 2004) and experimental (Li et al,2009;Toyama et al, 2004;Kudo et al, 2002;Schelbert et al, 2003) settings.…”