Vascular Network Formation on Macroporous Polydioxanone Scaffolds

Heene, Sebastian; Thoms, Stefanie; Kalies, Stefan; Wegner, Nils; Peppermüller, Pia; Born, Nikolai; Walther, Frank; Scheper, Thomas; Blume, Cornelia

doi:10.1089/ten.tea.2020.0232

Cited by 9 publications

(12 citation statements)

References 36 publications

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“…Capillary network on a macroporous PDO scaffold, generated by porogen‐leaching. (A) Poly‐p‐dioxanone scaffolds were produced by dissolving PDO in hexafluoro‐2‐propanol using a porogen leaching process with NaCl crystals as porogen [58,81]. (B) 1–4: 1) Macroporous PDO‐scaffold, 2) Dimensions of pores, 3) Tubule‐like‐structures out of GFP‐labeled HUVECs (green) and MSCs; cell nuclei stained with Hoechst 33342 (blue), 4) Lumen‐verification using a dextran assay (GFP‐HUVECs, green; dextran, red) [58]…”

Section: Tissue Engineering Trends In Vascular Scaffold Designmentioning

confidence: 99%

“…Increasingly, however, there F I G U R E 3 Capillary network on a macroporous PDO scaffold, generated by porogen-leaching. (A) Poly-p-dioxanone scaffolds were produced by dissolving PDO in hexafluoro-2-propanol using a porogen leaching process with NaCl crystals as porogen [58,81]. (B) 1-4: 1) Macroporous PDO-scaffold, 2) Dimensions of pores, 3) Tubule-like-structures out of GFP-labeled HUVECs (green) and MSCs; cell nuclei stained with Hoechst 33342 (blue), 4) Lumen-verification using a dextran assay (GFP-HUVECs, green; dextran, red) [58] is an awareness of the need for adapted CAD/computeraided manufacturing (CAM) and slicing software, and this is giving rise to corresponding research and development activities.…”

Section: D Printing For Generation Of Vascular Scaffoldsmentioning

confidence: 99%

“…(A) Poly-p-dioxanone scaffolds were produced by dissolving PDO in hexafluoro-2-propanol using a porogen leaching process with NaCl crystals as porogen [58,81]. (B) 1-4: 1) Macroporous PDO-scaffold, 2) Dimensions of pores, 3) Tubule-like-structures out of GFP-labeled HUVECs (green) and MSCs; cell nuclei stained with Hoechst 33342 (blue), 4) Lumen-verification using a dextran assay (GFP-HUVECs, green; dextran, red) [58] is an awareness of the need for adapted CAD/computeraided manufacturing (CAM) and slicing software, and this is giving rise to corresponding research and development activities. The incorporation of medical imaging for patient-specific scaffold development is also increasingly being explored in this context and must be optimized in the future [66].…”

Section: D Printing For Generation Of Vascular Scaffoldsmentioning

confidence: 99%

“…It could therefore be a good concept to combine a mechanically stable polymer such as PCL with a faster degrading polymer for surface structuring in the same scaffold. Such a polymer is, for example, represented by polydioxanone (PDO), that is very suitable for vascular cell seeding, and shall be discussed in the following [ 58 ].…”

Section: Tissue Engineering Trends In Vascular Scaffold Designmentioning

confidence: 99%

“…PDO has already been tested in combination with adipogenous tissue derived mesenchymal stem cells (AD‐MSCs) [ 59 ] and supported the differentiation of stem cells into bone and adipose tissue. PDO filaments can be used to produce macroporous scaffold by a porogen leaching method e. g. making use of NaCl as porogen (Figure 3A) [ 58 ]. These structured scaffolds can be integrated for cultivation of microvessels–as present as “vasa vasorum” in the vascular wall–to maintain nutrient supply for thicker arterial walls such as in the aorta.…”

Section: Tissue Engineering Trends In Vascular Scaffold Designmentioning

confidence: 99%

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Vascular implants – new aspects for in situ tissue engineering

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Conventional synthetic vascular grafts require ongoing anticoagulation, and autologous venous grafts are often not available in elderly patients. This review highlights the development of bioartificial vessels replacing brain-dead donoror animal-deriving vessels with ongoing immune reactivity. The vision for such bio-hybrids exists in a combination of biodegradable scaffolds and seeding with immune-neutral cells, and here different cells sources such as autologous progenitor cells or stem cells are relevant. This kind of in situ tissue engineering depends on a suitable bioreactor system with elaborate monitoring systems, three-dimensional (3D) visualization and a potential of cell conditioning into the direction of the targeted vascular cell phenotype. Necessary bioreactor tools for dynamic and pulsatile cultivation are described. In addition, a concept for design of vasa vasorum is outlined, that is needed for sustainable nutrition of the wall structure in large caliber vessels. For scaffold design and cell adhesion additives, different materials and technologies are discussed. 3D printing is introduced as a relatively new field with promising prospects, for example, to create complex geometries or micro-structured surfaces for optimal cell adhesion and ingrowth in a standardized and custom designed procedure. Summarizing, a bio-hybrid vascular prosthesis from a controlled biotechnological process is thus coming more and more into view. It has the potential to withstand strict approval requirements applied for advanced therapy medicinal products.

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Section: Tissue Engineering Trends In Vascular Scaffold Designmentioning

confidence: 99%

Section: D Printing For Generation Of Vascular Scaffoldsmentioning

confidence: 99%

Section: D Printing For Generation Of Vascular Scaffoldsmentioning

confidence: 99%

Section: Tissue Engineering Trends In Vascular Scaffold Designmentioning

confidence: 99%

Section: Tissue Engineering Trends In Vascular Scaffold Designmentioning

confidence: 99%

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Vascular implants – new aspects for in situ tissue engineering

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Biocompatible scaffolds based on collagen and oxidized dextran for endothelial cell survival and function in tissue engineering

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et al. 2023

Engineering in Life Sciences

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Angiogenesis is a vital step in tissue regeneration. Hence, the current study aimed to prepare oxidized dextran (Odex)/collagen (Col)‐hydrogels with laminin (LMN), as an angiogenic extracellular matrix (ECM) component, for promoting human umbilical vein endothelial cell (HUVEC) proliferation and function. Odex/Col scaffolds were constructed at various concentrations and temperatures. Using oscillatory rheometry, scanning electron microscopy (SEM), and cell viability testing, the scaffolds were characterized, and then HUVEC proliferation and function was compared with or without LMN. The gelation time could be modified by altering the Odex/Col mass ratio as well as the temperature. SEM showed that Odex/Col hydrogels had a more regular three‐dimensional (3D) porous structure than the Col hydrogels. Moreover, HUVECs grew faster in the Col scaffold (12 mg/mL), whereas the Odex (30 mg/mL)/Col (6 mg/mL) scaffold exhibited the lowest apoptosis index. Furthermore, the expression level of vascular endothelial growth factor (VEGF) mRNA in the group without LMN was higher than that with LMN, and the Odex (30 mg/mL)/Col (6 mg/mL) scaffold without LMN had the highest VEGF protein secretion, allowing the cells to survive and function effectively. Odex/Col scaffolds, with or without LMN, are proposed as a tissue engineering construct to improve HUVEC survival and function for angiogenesis.

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Progress of biodegradable polymer application in cardiac occluders

Xu,

Fa,

Yang

et al. 2023

J Biomed Mater Res

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Cardiac septal defect is the most prevalent congenital heart disease and is typically treated with open‐heart surgery under cardiopulmonary bypass. Since the 1990s, with the advancement of interventional techniques and minimally invasive transthoracic closure techniques, cardiac occluder implantation represented by the Amplazter products has been the preferred treatment option. Currently, most occlusion devices used in clinical settings are primarily composed of Nitinol as the skeleton. Nevertheless, long‐term follow‐up studies have revealed various complications related to metal skeletons, including hemolysis, thrombus, metal allergy, cardiac erosion, and even severe atrioventricular block. Thus, occlusion devices made of biodegradable materials have become the focus of research. Over the past two decades, several bioabsorbable cardiac occluders for ventricular septal defect and atrial septal defect have been designed and trialed on animals or humans. This review summarizes the research progress of bioabsorbable cardiac occluders, the advantages and disadvantages of different biodegradable polymers used to fabricate occluders, and discusses future research directions concerning the structures and materials of bioabsorbable cardiac occluders.

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Vascular Network Formation on Macroporous Polydioxanone Scaffolds

Cited by 9 publications

References 36 publications

Vascular implants – new aspects for in situ tissue engineering

Vascular implants – new aspects for in situ tissue engineering

Biocompatible scaffolds based on collagen and oxidized dextran for endothelial cell survival and function in tissue engineering

Progress of biodegradable polymer application in cardiac occluders

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