Vascular responsiveness of isolated, perfused basilar arteries in dogs and monkeys.

Abstract

A new model of isolated basilar arterial preparation was developed in the dog and monkey. Isolated arteries were perfused at a constant flow rate with Krebs solution and suspended in a bath at 3TC. By inserting the steel cannula into the artery, the space between the luminal wall of the artery and the cannula was narrowed enough to obtain a suitable perfusion pressure during the perfusion. The resting perfusion pressure was maintained at a constant level of 50-100 mmHg. The drug solution was intraluminally administered, and the response was obtained as changes in the perfusion pressure. Responses to 6 vasoactive substances (norepinephrine, 5-HT, PGF2a, histamine, ATP and KCl) were compared between simian and canine basilar arteries. It was demonstrated that in simian arteries, PGF2a was the strongest but 5-HT and norepinephrine induced slight vasoconstriction to the same degree, and in canine basilar arteries, 5-HT induced the strongest constriction but norepinephrine did not produce significant vasoconstriction, and KC1 induced marked constriction to the same degree at extremely large doses in both species. It was demonstrated that the cannula inserting method is useful to observe the responses of isolated basilar arteries. cannula inserting method ; isolated basilar artery ; simian cerebral artery ; dog cerebral artery Recently, a new model of isolated and perfused vascular preparation was developed by Hongo and Chiba (1983) and modified by Tsuji and Chiba (1984). In their preparation, the resting perfusion pressure was maintained between 50-100 mm Hg which was close to physiological arterial blood pressure throughout the experiment. The drug administration was performed on the endothelial side of the artery. By using this method, vascular responses to vasoactive substances were studied in several parts of relatively large arteries isolated (Hongo and Chiba 1983;Tsuji and Chiba 1984; Chiba and Tsukada 1984a, b ; Ito and Chiba 1984a, b). In the present experiments, we made an attempt to apply this method to the isolated cerebral artery in the dog and monkey, by using a modified cannula inserting method, and to compare effects of 6 different vasoactive substances

Characteristics of the Responses of Isolated and Perfused Canine Splenic Arteries to Vasoactive Substances and to Periarterially Electrical Stimulation

“…Since Hongo and Chiba (4) introduced the cannula insertion method in pharmacological and physiological studies, effects of vasoactive substances on the canine intermediate auricular, femoral, mesenteric, ophthalmic, basilar, coronary and lingual arteries had been reported by the use of this method (8,(16)(17)(18)(19)(20)(21)(22)(23). Observations on vasoconstrictive responses to a-adrenoceptor agonists, ATP and histamine indicate that the distributions of adrenergic, purinergic and histaminergic receptors in different arteries are not always same (16,17,(19)(20)(21).…”

“…Observations on vasoconstrictive responses to a-adrenoceptor agonists, ATP and histamine indicate that the distributions of adrenergic, purinergic and histaminergic receptors in different arteries are not always same (16,17,(19)(20)(21).…”

“…Several reports in dicated that there were a few discrepancies of pharma cological responses to vasoactive substances using the can nula insertion method (14,15). On the other hand, we have reported that the vasoconstrictive responses to a adrenoceptor agonists, ATP and histamine in different ar teries are not always same, which reflects the distributions of adrenergic, purinergic and histaminergic receptors in vascular smooth muscle cells (16)(17)(18)(19)(20)(21). Therefore, we in vestigated the discrepancy of vasoconstrictive responses to clonidine, and observed the vasoconstrictive responses to vasoactive agents and electrical nerve stimulation in iso lated and perfused canine splenic arteries.…”

Time-Dependent Effects of Extraluminally-Applied Oxyhemoglobin and Endothelial Removal on Vasodilator Responses in Isolated, Perfused Canine Basilar Arteries

“…The cannula-inserting method, which was originally developed by Hongo and Chiba [24] , has been modified by Tsuji and Chiba [25] for the examination of responsiveness of various smaller vessels, including isolated basilar arteries of monkeys or dogs [26][27][28]. In the present study, using the stainless steel cannula-inserting method, we report the time-dependent effects of extraluminally ap plied oxyHb and endothelial removal with intraluminal saponin [27,29,30] on the vas cular responses to ACh, A23187, thimerosal and SNP in isolated and perfused canine basilar arteries.…”

Heterogeneous vasomotor responses of anatomically distinct feline cerebral arteries

“…Other than differences based purely on the anatomical location of the artery, both neurotransmitter concentrations and the density of innervation may be a function of vessel calibre within the cerebrovascular bed; such is the case for 5-hydroxytryptamine (Edvinsson et al, 1983a;Scatton et al, 1985). Another characteristic of the cerebral arteries resides in the heterogeneity of the vascular responses to agonists and antagonists not only between species (Chiba & Tsuji, 1985;Hamel et al, 1985;Sasaki et al, 1985;Usui et al, 1985) but also between anatomically distinct vessels (Toda, 1976) and, further, between different segments of the same vessel in a given species (Toda et al, 1984).…”