Vascular-specific expression of GUS and GFP reporter genes in transgenic grapevine (Vitis vinifera L. cv. Albariño) conferred by the EgCCR promoter of Eucalyptus gunnii

Gago, Jorge; Grima‐Pettenati, Jacqueline; Gallego, Pedro Pablo Ferrer

doi:10.1016/j.plaphy.2011.02.005

Cited by 29 publications

(13 citation statements)

References 36 publications

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“…The quantitative fluorometric GUS assay revealed higher levels of GUS activity in stem and root compared to leaf tissues. This is in agreement with the earlier reports which suggested that the Eucalyptus gunnii CCR gene (EgCCR) promoter activity was highest in lignified organs (stem and root) than leaves of transgenic tobacco (Lacombe et al 2000) and transgenic grapevine (Gago et al 2011). The pattern of expression directed by the LlCCR promoter in transgenic tobacco plants clearly showed that the Leucaena promoter sequence is recognized by tobacco transcriptional factors, in spite of the evolutionary distance between these two species.…”

Section: Discussionsupporting

confidence: 92%

Isolation of cinnamoyl CoA reductase and cinnamyl alcohol dehydrogenase gene promoters from Leucaena leucocephala, a leguminous tree species, and characterization of tissue-specific activity in transgenic tobacco

Prashant

Sunita

Vavilala

et al. 2011

Plant Cell Tiss Organ Cult

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Promoter sequences of a 795 bp cinnamoyl CoA reductase (LlCCR) and 1,882 bp cinnamyl alcohol dehydrogenase (LlCAD) genes were isolated from Leucaena leucocephala, a leguminous tree species by genome walking, and analysed using bioinformatics tools. This revealed presence of cis-elements such as AC-boxes, XYLAT, WRKY, and MYB binding sites in addition to CAAT and TATA boxes. For functional characterization, each of LlCCR and LlCAD promoter sequences were fused to b-glucuronidase (GUS) reporter gene, immobilized into pBI101 plasmid, and introduced into tobacco via Agrobacterium tumefaciens strain LBA4404. Histochemical observations of transgenic lines indicated tissue-specific expression of GUS in the vascular tissues of leaves, stems, and roots. These results demonstrate that GUS expression driven by either LlCCR or LlCAD promoters were involved in lignifying tissues, and more specifically in differentiating xylem cells. This observed tissue-specific expression driven by either LlCCR or LlCAD promoters is sufficient for reducing the lignin content only in vascular tissues, thus overcoming the risks and challenges associated with down-regulation of lignin content in whole plants.

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Section: Discussionsupporting

confidence: 92%

Isolation of cinnamoyl CoA reductase and cinnamyl alcohol dehydrogenase gene promoters from Leucaena leucocephala, a leguminous tree species, and characterization of tissue-specific activity in transgenic tobacco

Prashant

Sunita

Vavilala

et al. 2011

Plant Cell Tiss Organ Cult

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“…EgCCR promoter showed high expression in the newly formed vascular tissues in stems, leaves, and petioles of transformed grapevine that could be useful for engineered resistance against vascular pathogens (Gago et al 2011 ).…”

Section: Grapevinementioning

confidence: 99%

Promoters for Transgenic Horticultural Plants

Smirnova

Tishchenko²,

Ermakov

et al. 2014

Abiotic Stress Biology in Horticultural Plants

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Gene engineering provides an opportunity to obtain plants with either silenced or overexpressed target genes. This approach is frequently used to study various aspects of the biochemistry, physiology, and stress tolerance of horticultural plants. Selection of appropriate promoters to govern transcription of a transgenic construct is an important step in the development of effi cient genetic models. Here we present a brief overview of available literature data on the promoters investigated with the transgenic horticultural plants and some valuable information resources in this fi led.

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“…Genetic engineering offers new tools for studies that address the functional characterization of genes of interest in grapevine. Several groups have published reports on the transformation of Vitis [33][34][35][36][37][38][39][40]. To date, Gollop et al [41] have provided evidence for the stable transformation of non-embryogenic cultures (NEC).…”

Section: Introductionmentioning

confidence: 99%

A reliable protocol for the stable transformation of non-embryogenic cells cultures of grapevine (Vitis vinifera L.) and Taxus x media

et al. 2015

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One of the major intent of metabolic engineering in cell culture systems is to increase yields of secondary metabolites. Efficient transformation methods are a priority to successfully apply metabolic engineering to cell cultures of plants that produce bioactive or therapeutic compounds, such as Vitis vinifera and Taxus x media. The aim of this study was to establish a reliable method to transform non-embryogenic cell cultures of these species. The V. vinifera cv. Gamay/cv. Monastrell cell lines and Taxus x media were used for Agrobacterium-mediated transformation using the Gateway-compatible Agrobacterium sp. binary vector system for fast reliable DNA cloning. The Taxus x media and Vitis cell lines were maintained in culture for more than 4 and 15 months, respectively, with no loss of reporter gene expression or antibiotic resistance. The introduced genes had no discernible effect on cell growth, or led to extracellular accumulation of phytoalexin trans-Resveratrol (t-R) in response to elicitation with methylated cyclodextrins (MBCD) and methyl jasmonate (MeJA) in the grapevine transgenic cell lines compared to the parental control. The method described herein provides an excellent tool to exploit exponentially growing genomic resources to enhance, optimize or diversify the production of bioactive compounds generated by grapevine and yew cell cultures, and offers a better understanding of many grapevine and yew biology areas.

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Vascular-specific expression of GUS and GFP reporter genes in transgenic grapevine (Vitis vinifera L. cv. Albariño) conferred by the EgCCR promoter of Eucalyptus gunnii

Cited by 29 publications

References 36 publications

Isolation of cinnamoyl CoA reductase and cinnamyl alcohol dehydrogenase gene promoters from Leucaena leucocephala, a leguminous tree species, and characterization of tissue-specific activity in transgenic tobacco

Isolation of cinnamoyl CoA reductase and cinnamyl alcohol dehydrogenase gene promoters from Leucaena leucocephala, a leguminous tree species, and characterization of tissue-specific activity in transgenic tobacco

Promoters for Transgenic Horticultural Plants

A reliable protocol for the stable transformation of non-embryogenic cells cultures of grapevine (Vitis vinifera L.) and Taxus x media

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