Vascular Thymus Transplantation in Rats

Kampinga, J; Schuurman, Henk Jan; Pol, Gerda H.; Bartels, H.; Broekhuizen, Roel; Vaessen, L. M. B.; Tielen, Frans; Rozing, Jan; Blaauw, Egbert H.; Roser, Bruce; Aspinall, Richard; Nieuwenhuis, Paul

doi:10.1097/00007890-199010000-00028

Cited by 19 publications

(5 citation statements)

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“…lymphoid organs. T o establish the relationships between these 11 different subsets we used the method of vascular thymus transplantation in RT7 congenic rats [12], and followed the phenotypic changes of donor thymic emigrants during their sojourn in the lymphoid organs. Based on these, and additional short-time thymectomy experiments, a putative pathway of post-thymic T cell development was postulated [6].…”

Section: Introductionmentioning

confidence: 99%

Post-thymic T cell development in rats: an update

Kampinga

Groen

Klatter

et al. 1992

Biochemical Society Transactions

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Section: Introductionmentioning

confidence: 99%

Post-thymic T cell development in rats: an update

Kampinga

Groen

Klatter

et al. 1992

Biochemical Society Transactions

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“…The development of appropriate animal models has been crucial for many of the major advances in transplantation. Although the rat vascular thymus transplantation model was reported by Kampinga et al, 1 this is the first description of mouse vascular thymus transplantation. The mouse as an animal model has several advantages over the rat.…”

Section: Discussionmentioning

confidence: 99%

“…Thymus transplantation has been used extensively to investigate various aspects of thymus function, as summarized by Kampinga et al 1 and others: the attraction and intrathymic maturation of bone marrow, 2,3 the development of major histocompatibility complex (MHC) restriction, 4,5 and induction of tolerance. 6,7 The role of the thymus has also been studied in infections, 8,9 autoimmune disease, 10,11 hypertension, 12 malignant disorders, 13,14 and congenital immune deficiency disease in the mouse, 15,16 rat, 17 and human.…”

Section: Microsurgery 19:56-60 1999mentioning

confidence: 99%

“…Furthermore, nonvascular grafts failed to reproduce the normal anatomy of the thymus, and they may not have resulted in enough T-cells. To address these problems, Kampinga et al 1 developed a vascular thymus transplantation technique in the rat in 1990. In this model, ischemia damage was avoided because the vascular anastomosis between donor and recipient was well established.…”

Section: Microsurgery 19:56-60 1999mentioning

confidence: 99%

“…The donor surgery was modified from the rat vascular thymus transplant model. 1 A midline abdominal incision was made in the donor animal. After ligating the aorta and inferior vena cava below the renal artery and vein, a 30gauge needle was introduced into the inferior vena cava.…”

Section: Donor Operationmentioning

confidence: 99%

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Surgical technique for vascularized thymus transplantation in mice

et al. 1999

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Traditionally, mouse nonvascularized thymus implants have been used to investigate various aspects of thymus function. However, these grafts are easily damaged by ischemia and fail to reproduce the normal anatomy of the thymus. In addition, the function of these grafts has not been fully examined. We have recently developed a vascularized thymus transplant model in mice. The donor operation consists of isolating the right lobe of the thymus and creating a single vascular pathway. In the recipient surgery, end-to-side anastomoses between donor brachycephalic artery and recipient right common carotid artery, and between donor superior caval vein and recipient right external jugular vein, were performed. We performed 10 consecutive isografts in BALB/c mice with a success rate of 90%. The thymus grafts had a normal histology and function. This study illustrates that it is technically possible to transplant a mouse vascular thymus graft. This model has several advantages that make it a useful tool to study many aspects of thymus function. We plan to use this model further to study the potential for induction of tolerance by thymus grafts.

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Development of donor‐specific chimerism and tolerance in composite tissue allografts under αβ‐T‐cell receptor monoclonal antibody and cyclosporine a treatment protocols

et al. 2004

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Recently, we induced donor-specific tolerance to rat hindlimb allografts under a 35-day course of alphabeta-T-cell receptor monoclonal antibody (alphabeta-TCR mAb) and cyclosporine A (CsA). In this report, we investigated the role of shorter alphabeta-TCR/CsA protocols on tolerance induction. We performed 52 hindlimb transplantations, between Lewis-Brown-Norway (LBN, F1) donors and Lewis recipients to test the impact of 21-, 7-, and 5-day protocols of combined alphabeta-TCR/CsA treatment on tolerance induction. Donor-specific tolerance and immunocompetence were tested by mixed lymphocyte reaction (MLR) in vitro and by standard skin grafting in vivo. The efficacy of immunosuppressive protocol and donor-specific chimerism was assessed by flow cytometry. All transplants under 5, 7, and 21 days of combined alphabeta-TCR/CsA therapy survived over 350 days. Clinical tolerance and immunocompetence were confirmed by skin grafting in vivo and MLR in vitro. Flow cytometry revealed a high level of donor chimerism in the peripheral blood of long-term survivors. The extension of survival of limb allografts and allo-unresponsiveness were directly associated with the development of stable chimerism in the tolerant recipients.

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Vascular Thymus Transplantation in Rats

Cited by 19 publications

References 0 publications

Post-thymic T cell development in rats: an update

Post-thymic T cell development in rats: an update

Surgical technique for vascularized thymus transplantation in mice

Development of donor‐specific chimerism and tolerance in composite tissue allografts under αβ‐T‐cell receptor monoclonal antibody and cyclosporine a treatment protocols

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