Vascular tissue engineering: microtextured scaffold templates to control organization of vascular smooth muscle cells and extracellular matrix

Sarkar, Sumona; Dadhania, Manisha; Rourke, P. M. C.; Desai, Tejal A.; Wong, Joyce Y.

doi:10.1016/j.actbio.2004.08.003

Cited by 119 publications

(112 citation statements)

References 20 publications

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“…In cells cultured on 3D patterned scaffolds, the expression levels of adhesion signalling complexes, 43 focal adhesion kinase 13,43 and F-actin, 13,15,44 their proliferative, 8,13,20,22 cell aggregation [17][18][19][20] and metabolic 13,[19][20][21] capacities, and their differentiation potential, 9, 23 differ from those of cells cultured on flat surfaces.…”

Section: Discussionmentioning

confidence: 99%

Influence of the pattern size of micropatterned scaffolds on cell morphology, proliferation, migration and F-actin expression

2014

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To determine how the three-dimensional (3D) shape of scaffolds influences cell functions, 3D micropatterned scaffolds of various sizes were fabricated on a silicon substrate. The micropatterns were equilateral triangular pores with 3-20 µm long sides, and all had the same pore ratio (total pore area per unit area) and depth. The patterns only differed in terms of their 2D size. Such scaffolds have not been previously generated, and thus the effects of pattern size on cell functions have not been addressed. NIH-3T3 cells were cultured on these micropatterned scaffolds, and their morphology, proliferation rate, migration rate, and level of F-actin expression were assessed. Cells became more rounded and F-actin expression decreased as the pattern size of the scaffold decreased. Relationships were also demonstrated between pattern size and cell proliferation and migration. These results suggest that the pattern size of 3D micropatterned scaffolds affects the level of mechanical stress that cells experience, and thereby influences F-actin expression, cell morphology, cell proliferation and cell migration.2

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Section: Discussionmentioning

confidence: 99%

Influence of the pattern size of micropatterned scaffolds on cell morphology, proliferation, migration and F-actin expression

2014

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“…Some studies have examined cellular responses to the stiffness of different substrates, such as polyacrylamide (PAAM) (Lo et al 2000;Wang et al 2000;Engler et al 2004a;Stroka & Aranda-Espinoza 2011a;Galie et al 2015), Polydimethylsiloxane (PDMS) (Sarkar et al 2005;Ataollahi et al 2015) or alginate (Huang et al 2013), as such materials are biocompatible and can be fabricated using suitable methods to achieve desired stiffness values.…”

Section: Introductionmentioning

confidence: 99%

Regulation of Endothelial Cell Adherence and Elastic Modulus by Substrate Stiffness

Jalali

Tafazzoli-Shadpour

Haghighipour

et al. 2015

Cell Communication & Adhesion

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Although substrate stiffness has been previously reported to affect various cellular aspects, such as morphology, migration, viability, growth, and cytoskeletal structure, its influence on cell adherence has not been well examined. Here, we prepared three soft, medium, and hard polyacrylamide (PAAM) substrates and utilized AFM to study substrate elasticity and also the adhesion and mechanical properties of endothelial cells in response to changing substrate stiffness. Maximum detachment force and cell stiffness were increased with increasing substrate stiffness. Maximum detachment force values were 0.28 ± 0.14, 0.94 ± 0.27, and 1.99 ± 0.59 nN while Young's moduli of cells were 218. 85 ± 38.73, 385.58 ± 131.67, and 933.20 ± 428.92 Pa for soft, medium, and hard substrates, respectively. Human umbilical vein endothelial cells (HUVECs) showed round to more spread shapes on soft to hard substrates, with the most organized and elongated actin structure on the hard hydrogel. Our results confirm the importance of substrate stiffness in regulating cell mechanics and adhesion for a successful cell therapy. ARTICLE HISTORY

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“…Soft lithography uses a stamp or mold prepared by casting an elastomer, typically polydimethylsiloxane (PDMS), against a master containing a patterned relief structure prepared via traditional photolithography techniques. These materials can then serve as stamps that can be used to deposit molecules in precise patterns via microcontact printing in order to spatially control cell adhesion [29,31], as well as being used as topological cues themselves, mimicking the 3-dimensional nano-and microstructure of the native ECM [34]. A particularly attractive use for these molds is for hot embossing of thermoplastic polymers (e.g., polystyrene (PS)) in order to create substrates with topological features on the micron-and submicron-scale [32,37,42].…”

Section: Introductionmentioning

confidence: 99%

A thermoresponsive, microtextured substrate for cell sheet engineering with defined structural organization

et al. 2008

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The proper function of many tissues depends critically on the structural organization of the cells and matrix of which they are comprised. Therefore, in order to engineer functional tissue equivalents that closely mimic the unique properties of native tissues it is necessary to develop strategies for reproducing the complex, highly organized structure of these tissues. To this end, we sought to develop a simple method for generating cell sheets that have defined ECM/cell organization using microtextured, thermoresponsive polystyrene substrates to guide cell organization and tissue growth. The patterns consisted of large arrays of alternating grooves and ridges (50 μm wide, 5 μm deep). Vascular smooth muscle cells cultured on these substrates produced intact sheets consisting of cells that exhibited strong alignment in the direction of the micropattern. These sheets could be readily transferred from patterned substrates to non-patterned substrates without the loss of tissue organization. Ultimately, such sheets will be layered to form larger tissues with defined ECM/cell organization that spans multiple length scales.

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Vascular tissue engineering: microtextured scaffold templates to control organization of vascular smooth muscle cells and extracellular matrix

Cited by 119 publications

References 20 publications

Influence of the pattern size of micropatterned scaffolds on cell morphology, proliferation, migration and F-actin expression

Influence of the pattern size of micropatterned scaffolds on cell morphology, proliferation, migration and F-actin expression

Regulation of Endothelial Cell Adherence and Elastic Modulus by Substrate Stiffness

A thermoresponsive, microtextured substrate for cell sheet engineering with defined structural organization

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