Vascular Tissue Preservation Techniques

Müller‐Schweinitzer, Else

doi:10.1007/978-1-4612-0303-2_24

Cited by 7 publications

(4 citation statements)

References 27 publications

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“…Whilst this discrepancy might be due to species differences, it may also reflect different susceptibility of endothelial cells from different calibre arteries to the explantation, cryopreservation or thawing process. Furthermore, the reduction in SMC contractile force after cryopreservation might be explained by the prolonged ( > 10 min) exposure to cryomedium (DMSO) before cryopreservation [22]. Further studies are required to further optimise this routinely used cryopreservation protocol.…”

Section: Resultsmentioning

confidence: 99%

Impact of current cryopreservation procedures on mechanical and functional properties of human aortic homografts

et al. 2001

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We evaluated the impact f standard cryopreservation on mechanical and functional properties of human aortic homografts. From 14 human heart-valve donors, the thoracic descending aorta was obtained. Effects of cryopreservation on mechanical (elastic properties and breaking stress) and smooth muscle cell (SMC) and endothelium function were tested. Cryopreservation (cryo) did not significantly affect Young's modulus of elastin (fresh: 3.1 +/- 1.0, cryo: 2.7 +/- 0.9 x 10(5) Nm(-2)), collagen recruitment pressure (fresh: 1.1 +/- 0.3, cryo: 1.1 +/- 0.4 x 10(4) Nm(-2)), distensibility (fresh: 3.8 +/- 1.8, cryo: 3.6 +/- 1.6 x 10(5) N(-1)m2), or breaking stress (fresh: 2.4 +/- 1.0, cryo: 2.2 +/- 1.0 x 10(6) Nm(-2)). Following explantation, no endothelium-dependent relaxation was found. SMC function and endothelium-independent relaxation were mainly intact after explantation but significantly decreased after cryopreservation. Aortic mechanical properties are not influenced by cryopreservation. Following explantation, almost no endothelial cell function is present, and SMC contractility is strongly affected after cryopreservation.

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Section: Resultsmentioning

confidence: 99%

Impact of current cryopreservation procedures on mechanical and functional properties of human aortic homografts

et al. 2001

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“…The various advantages of the cryopreservation technique for storage of vascular tissues for subsequent pharmacological studies have been highlighted in recent reviews (Muller-Schweinitzer, 1992, 1994a. Except for a few reports on human airway epithelium (Raeburn et al 1986;Aizawa et al 1988;Fernandes et al 1989Fernandes et al , 1990, most in vitro studies on airway epithelial function are routinely carried out with animal tissues, largely because tissues from humans (normally excised following surgery) are not readily available for experimental studies.…”

Section: Discussionmentioning

confidence: 99%

“…This, however, only allows storage for a few days, whereas cryopreservation in liquid nitrogen allows storage of blood vessels indefinitely and a wide variety of functional activities are well preserved in these tissues (Miiller-Schweinitzer, 1992, 1994a. In addition, it has been shown that the same technique also preserves the functional activity of human and porcine airway smooth muscle (Johnson, McKay, Alouan, Armour & Black, 1993; Miiller-Schweinitzer, Hasse & Swoboda, 1993a, b).…”

Section: Introductionmentioning

confidence: 99%

Evidence for preservation of epithelial function in cryopreserved porcine and human bronchi

Vacciana¹,

Ebeigbe²,

Hasse³

et al. 1994

Experimental Physiology

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SUMMARYPorcine and human bronchi have been investigated in vitro without or after storage at -196°C in Krebs-Henseleit solution containing 2-0 M dimethyl sulphoxide and 01 M sucrose as cryoprotectants. In bronchi from both species maximal post-thaw contractile responses to acetylcholine (ACh) were reduced by about 25-30 % compared to unfrozen bronchi. To assess the viability of bronchi and endothelium-denuded rat aortic strips, was employed. The release of an epithelium-derived inhibitory factor (EpDIF) was induced by ACh and assessed in terms of concentration-dependent relaxation of the endothelium-denuded rat aortae. Following removal of bronchial epithelium, ACh failed to elicit any relaxation of rat aorta. With cryopreserved bronchi from both pig and human about 5 and 30 times higher concentrations of ACh, respectively, were required to elicit the same relaxant response as with unfrozen bronchi. The results suggest that after the freezing-thawing process both smooth muscle and epithelial function is largely preserved and provide support for the use of cryopreservation for storage of airway preparations for pharmacological studies.

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“…Cryopreserved human blood vessels may become important tools in both bypass surgery and peripheral vascular reconstruction in patients without sufficient autologous graft material ( Brockbank, 1995 ; Fujitani et al ., 1992 ) and are actually interesting topics of pharmacological research ( Müller‐Schweinitzer, 1994a , 1994b ; 1998 ; Müller‐Schweinitzer & Fozard, 1997 ). However, evidence has been presented that after thawing of cryostored human arteries the compliance of the blood vessel is diminished ( Müller‐Schweinitzer, 1994a , 1994b ) while at the same time the sensitivity of smooth muscle cells to contractile stimuli may be enhanced as indicated by a leftward shift of the concentration‐response curves to various agonists in human coronary, mesenteric and internal mammary arteries ( Müller‐Schweinitzer et al ., 1997 ; 1998 ). Previous experiments on human internal mammary arteries have suggested that cryopreservation facilitates contractile responses induced by activation of protein kinase C (PKC) which seems to be associated with increased Ca 2+ influx through dihydropyridine‐sensitive Ca 2+ channels.…”

Section: Introductionmentioning

confidence: 99%

The mechanism of cryoinjury: In vitro studies on human internal mammary arteries

Müller‐Schweinitzer

Brett

Zerkowski

et al. 2000

British J Pharmacology

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1 The mechanism of cryoinjury was investigated in human internal mammary arteries (IMA) by monitoring contractile responses to ET-1 and KCl. 2 For cryopreservation segments of IMA were equilibrated for 20 min with the cryomedium (RPMI 1640 culture medium containing 1.8 M DMSO and 0.1 M sucrose), frozen at a mean cooling rate of 1.38C min 71 to 7708C and stored in liquid nitrogen. Before use, samples were thawed slowly and the cryomedium removed by dilution. 3 Compared to unfrozen controls, ET-1 stimulated frozen/thawed IMA with similar e cacy but at 3 fold lower concentrations (P50.001). Addition of ET-1 (100 nM) induced maximal contraction of unfrozen IMA within 10 min, declining thereafter to 25% after 90 min. In frozen/thawed IMA the ET-1-induced contraction was sustained but could be reversed if protein kinase C was blocked by staurosporine (100 nM). Responses to ET-1 of cryostored IMA were 5 fold more susceptible to blockade by nifedipine than those of controls. 4 After cryostorage the e cacy of KCl was diminished to 40% (P50.05) and the KCl curve was shifted to the left (2 fold, P50.001). In both unfrozen and cryostored IMA the KCl (60 mM) e ect was sustained and equally susceptible to nifedipine. 5 It is suggested that the smooth muscle cell of IMA is receptive to physical forces which occur during cryopreservation. These forces modify transmembrane signal transduction and intracellular pathways, that are common to pharmacological agonists thereby changing vascular responses to several contractile agonists after thawing. British Journal of Pharmacology (2000) 130, 636 ± 640 Keywords: Human internal mammary artery; cryopreservation; cryoinjury; endothelin-1; depolarization; protein kinase C activation; calcium in¯ux; staurosporine; nifedipine Abbreviations: ANOVA, one-way analysis of variance; DAG, diacylglycerol; DMSO, dimethylsulphoxide; EC 50 , concentration of an agonist producing 50% of E max ; EDTA, ethylenediaminetetraacetatic acid disodium salt; E max , maximal contractile e ect; ET-1, endothelin-1; IC 50 , drug concentration of an inhibitor producing 50% inhibition of E max of an agonist; IMA, internal mammary artery; IP 3 , phosphoinositide 4,5-trisphosphate; KH, Krebs-Henseleit; pD 2 , negative logarithm of the molar concentration of agonist producing 50% of E max ; PGF 2a , prostaglandin

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Vascular Tissue Preservation Techniques

Cited by 7 publications

References 27 publications

Impact of current cryopreservation procedures on mechanical and functional properties of human aortic homografts

Impact of current cryopreservation procedures on mechanical and functional properties of human aortic homografts

Evidence for preservation of epithelial function in cryopreserved porcine and human bronchi

The mechanism of cryoinjury: In vitro studies on human internal mammary arteries

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