Vascular wilt of basil in Australia

Summerell, Brett A.; Gunn, L. V.; Bullock, Stephen H.; Tesoriero, L.; Burgess, L. W.

doi:10.1071/ap05100

Cited by 12 publications

(6 citation statements)

References 11 publications

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“…The clonal origin of the forma specialis (Katan et al., 1996) and the presence of the sequence used for designing the real‐time PCR primers in the recently identified populations of F. oxysporum f. sp. basilici in South Africa and Australia (Swart and van Niekerk, 2003; Summerell et al., 2006), suggest that the primers–probe combination should also amplify F. oxysporum f. sp. basilici isolates originating from other localities.…”

Section: Resultsmentioning

confidence: 99%

“…Sweet basil production is an important source of revenue for regional economies. The disease has been reported in Italy (Tamietti and Matta, 1989), Israel (Katan et al., 1996), the USA (Datnoff et al., 1997), Spain (Guirado‐Moya et al., 2002), Japan (Taba et al., 2002), South Africa (Swart and van Niekerk, 2003), Greece (Biris et al., 2004) and Australia (Summerell et al., 2006). Only one single clonal population belonging to VCG 0400 has been identified thus far (Katan et al., 1996).…”

Section: Introductionmentioning

confidence: 99%

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Development of a Real‐time Polymerase Chain Reaction for the Detection of Fusarium oxysporum f. sp. basilici from Basil Seed and Roots

Pasquali

Piatti²,

Gullino³

et al. 2006

Journal of Phytopathology

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A real-time polymerase chain reaction (PCR) assay using a TaqMan Ò probe was developed to detect the causal agent of wilt and crown rot of basil from infected plants and seed in Italy. The aim of the study was to diminish testing time, previously performed using nested-PCR, and to create the conditions for future automation. The sensitivity of the assay was shown to be similar to the detection limit of the available nested-PCR procedure. The advantages of real-time PCR system include halving of the testing time, as well as the ability to identify both internally and externally infected seed to the sensitivity of 1 pg of genomic DNA. The assay was able to detect the presence of the pathogen in infected seed up to a sensitivity of 24 (SD: ±10) CFU per 100 seeds.www.blackwell-synergy.com

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of a Real‐time Polymerase Chain Reaction for the Detection of Fusarium oxysporum f. sp. basilici from Basil Seed and Roots

Pasquali

Piatti²,

Gullino³

et al. 2006

Journal of Phytopathology

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“…The variability of FOB isolates has already been identified either at DNA level or by the presence of distinct compatibility groups (for a recent review, see Summerell et al 2006). However, there is yet no clear indication of the potential presence of distinct physiological races among FOB isolates.…”

Section: Discussionmentioning

confidence: 96%

“…The pathogen has a narrow host range and is considered specific for basil (Keinath 1994;Garibaldi et al 1997;Reuveni et al 1997). FOB has been disseminated to new growing areas throughout the world via imported seeds (Elmer 2001) and is now present in most countries where sweet basil is cultivated (Grasso 1975;Wick and Haviland 1992;Katan et al 1994;Keinath 1994;Garibaldi et al 1997;Guirado-Moya et al 2002;Swart and van Niekerk 2003;Summerell et al 2006).…”

Section: Introductionmentioning

confidence: 97%

Search for sources of wide-spectrum resistance to Fusarium oxysporum f. sp. basilici isolates in accessions of Ocimum species

2008

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Ocimum (Lamiaceae) is an important plant genus, with many species used for food flavorings and for essential oils. Fusarium wilt (Fusarium oxysporum f. sp. basilici, FOB) is the most important disease of basil (O. basilicum L.). Twenty-five accessions of O. basilicum, five of O. americanum and two of O. campechianum were initially evaluated for resistance to one FOB isolate (named as FOB-1). Eight accessions (identified as resistant to FOB-1) and one susceptible control were reevaluated to FOB-1 and four other FOB isolates of distinct geographic origins. The FOB isolates varied in aggressiveness and interacted differentially with the Ocimum accessions. Two accessions of O. americanum, one of O. campechianum, and one of O. basilicum had high levels of resistance to all five FOB isolates. The Ocimum germplasm identified here could represent useful sources of resistance genes for developing cultivars with wide-spectrum resistance (i.e., effective against a broad range of FOB isolates). In addition, having a set of potential differential accessions might be useful for large-scale analysis of FOB isolates to demonstrate the presence of physiological races in the Ocimum-FOB pathosystem.

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“…Leaf and stem samples were washed with running water thoroughly and approximately, 4 × 7 mm sized small tissue segments cut from active tissue margins of lesions. Small symptomatic pieces of samples taken from leaves and stems were surface sterilized by 1% sodium hypochlorite solution (laundry bleach) for one minute, 70% alcohol for one minute and rinsed in sterile distilled water three times of one minute each (Geiser et al, 2005;Summerell et al, 2006) and the prepared samples were macerated in sterile distilled water and crashed using laboratory mortar and pestle. Finally, the filtrates were diluted using sterile distilled water and wait for 30 min and allowed the bacteria to float and release from the tissues and then each suspension of the plates were streaked with sterile wire loop on the agar media.…”

Section: Isolation Of Bacterial Brown Spotmentioning

confidence: 99%

Assessment and characterization of mung bean (Vigna radiata) bacterial brown spot in Eastern Amhara, Ethiopia

Teferie¹,

Admasu²,

Damessa³

2020

Afr. J. Agric. Res.

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Mung bean is one of the major early maturing pulse crop grown all over the world including Ethiopia. The production of the crop in Ethiopia, however, suffers from many diseases caused by bacteria. The study aims to assess the intensity and identify the major foliar bacterial and fungal pathogens of the crop. Purposively, 3 districts and randomly 90 mung bean fields were surveyed during the study period. Pathogenicity test, macroscopic and microscopic observations and biochemical tests were used for identification. Symptomatic of 33 diseased bacterial samples were initially isolated and purified on nutrient agar. Bacterial brown spot was found as important foliar devastating identified diseases, even if its distribution varied among localities. Water soaked, small, circular, brown lesions surrounded by yellow zones were observed in all bacterial brown spot isolates after 8 days of inoculation. Based on cultural and biochemical characteristics, bacterial isolates were identified as grams negative phytopathogenic bacteria called Pseudomonas syringae pv. Syringae. However, further characterization of both isolates and phenotypic characteristics of a large population of newly emerged P. syringae pv. Syringae from various host plants should capture the research attention. This is the first report on the occurrence of such disease in Ethiopia.

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Vascular wilt of basil in Australia

Cited by 12 publications

References 11 publications

Development of a Real‐time Polymerase Chain Reaction for the Detection of Fusarium oxysporum f. sp. basilici from Basil Seed and Roots

Development of a Real‐time Polymerase Chain Reaction for the Detection of Fusarium oxysporum f. sp. basilici from Basil Seed and Roots

Search for sources of wide-spectrum resistance to Fusarium oxysporum f. sp. basilici isolates in accessions of Ocimum species

Assessment and characterization of mung bean (Vigna radiata) bacterial brown spot in Eastern Amhara, Ethiopia

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