Vascularized Tissue-Engineered Ears

Neumeister, Michael W.; Wu, Tammy; Chambers, Christopher B.

doi:10.1097/01.prs.0000195071.01699.ce

Cited by 59 publications

(40 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Hott et al [18] used computer-aided design and injection molding technologies to fabricate small, precisely shaped chondrocyte-seeded calcium alginate structures in the shape of butterfly tympanic membrane patches. Neumeister et al [19] used silicone mold placed over transposed vessels in the abdominal wound pocket of the rat to form and shape vascularized molded fibrous capsule, which was later filled with chondrocytes and a fibrin glue carrier. Kamil at al.…”

Section: Discussionmentioning

confidence: 99%

Mold-Shaped, Nanofiber Scaffold-Based Cartilage Engineering Using Human Mesenchymal Stem Cells and Bioreactor

Janjanin

Morgan

et al. 2008

Journal of Surgical Research

View full text Add to dashboard Cite

Background-Mesenchymal stem cell (MSC)-based tissue engineering is a promising future alternative to autologous cartilage grafting. This study evaluates the potential of using MSCs, seeded into electrospun, biodegradable polymeric nanofibrous scaffolds, to engineer cartilage with defined dimensions and shape, similar to grafts used for subcutaneous implantation in plastic and reconstructive surgery.

show abstract

Section: Discussionmentioning

confidence: 99%

Mold-Shaped, Nanofiber Scaffold-Based Cartilage Engineering Using Human Mesenchymal Stem Cells and Bioreactor

Janjanin

Morgan

et al. 2008

Journal of Surgical Research

View full text Add to dashboard Cite

show abstract

“…In contrast, only a few studies have been published dealing with the prefabrication of in vitro cartilage-engineered scaffolds to ensure survival, function and shape in vivo (Staudenmaier et al 2004, Neumeister et al 2006). Shen was the first to describe the term "prefabrication" (Shen 1982), meaning the implantation of a vascular pedicle under a skin flap.…”

Section: Introductionmentioning

confidence: 91%

Vascularization of engineered cartilage constructs in a mouse model

et al. 2014

View full text Add to dashboard Cite

Tissue engineering of cartilage tissue offers a promising method for reconstructing ear, nose, larynx and trachea defects. However, a lack of sufficient nutrient supply to cartilage constructs limits this procedure. Only a few animal models exist to vascularize the seeded scaffolds. In this study, polycaprolactone (PCL)-based polyurethane scaffolds are seeded with 1 × 10(6) human cartilage cells and implanted in the right hind leg of a nude mouse using an arteriovenous flow-through vessel loop for angiogenesis for the first 3 weeks. Equally seeded scaffolds but without access to a vessel loop served as controls. After 3 weeks, a transposition of the vascularized scaffolds into the groin of the nude mouse was performed. Constructs (verum and controls) were explanted 1 and 6 weeks after transposition. Constructs with implanted vessels were well vascularized. The amount of cells increased in vascularized constructs compared to the controls but at the same time noticeably less extracellular matrix was produced. This mouse model provides critical answers to important questions concerning the vascularization of engineered tissue, which offers a viable option for repairing defects, especially when the desired amount of autologous cartilage or other tissues is not available and the nutritive situation at the implantation site is poor.

show abstract

“…Neumeister et al combined the techniques of vascular prefabrication, tissue culturing, and capsule formation to fabricate ear construct that is reliably transferable on its blood supply. [36] Despite of the encouraging progress, many improvements are still needed for its clinical application, such as to shorten the in vitro expansion time, to strengthen the mechanical property of the neocartilage, and to simplify the whole process.…”

Section: Cartilage Tissue Engineeringmentioning

confidence: 99%