Vasculogenesis and angiogenesis in the endometrium during menstrual cycle and implantation

Demir, Ramazan; Yaba, Aylin; Huppertz, Berthold

doi:10.1016/j.acthis.2009.04.004

Cited by 168 publications

(156 citation statements)

References 89 publications

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“…In addition, VEGFA was expressed in the mouse and rabbit endometrium and likely participated in the increased angiogenesis and vascular permeability necessary for implantation [44,45]. Evidence suggested that the expression of VEGFA was highly regulated in a temporal and spatial manner at the early stage of implantation [46]. HOXA10, a homeobox-containing transcription factor, expressed cyclically during the menstrual cycle in the endometrium under the influence of steroid hormone, and had the highest expression level during WOI [47,48].…”

Section: Mir-181a Might Participate In the Formation Of Endometrial Rmentioning

confidence: 99%

WITHDRAWN: Endometrial epithelial cell apoptosis is inhibited via a ciR8073-miR181a-neurotensis pathway during embryo implantation

Zhang¹,

Liu²,

Liu³

et al. 2018

Oncotarget

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Development of receptive endometrium (RE) from pre-receptive endometrium (PE) is essential for embryo implantation, but the molecular mechanisms are not fully understood. In this study, lncRNA/circRNA-miRNA-mRNA networks were constructed to explore the functions of potential competing endogenous RNAs (ceRNA) during the development of RE in dairy goats. We observed that circRNA8073 (ciR8073) decreased levels of miR-181a by acting as a miRNA sponge. This effect indirectly increased expression of neurotensin in endometrial epithelial cells (EECs). Neurotensin in turn inhibited EEC apoptosis by increasing expression of BCL-2/BAX via the MAPK pathway, and also increased expression leukemia-inhibitory factor, cyclo-oxygenase 2, vascular endothelial growth factor A and homeobox A10. We have thus identified a ciR8073-miR181a-neurotensin pathway in the endometrium of dairy goats. Via this pathway, ciR8073 functions as a ceRNA that sequesters miR-181a, thereby protecting neurotensin transcripts from miR-181a-mediated suppression in EECs.

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Section: Mir-181a Might Participate In the Formation Of Endometrial Rmentioning

confidence: 99%

WITHDRAWN: Endometrial epithelial cell apoptosis is inhibited via a ciR8073-miR181a-neurotensis pathway during embryo implantation

Zhang¹,

Liu²,

Liu³

et al. 2018

Oncotarget

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“…This is probably because sub-epithelial stromal cells underwent transformation to other cell types or subjected to an increase in proliferation rate when implantation occurs. It could therefore be suggested that reduction in expression of SM22 alpha in sheep intercaruncular tissue at the time of implantation is probably due to endometrial cell differentiation while it is widely expressed in endothelial cells contributing to new formation of microvasculature at that time (Demir et al 2009). …”

Section: Actin Binding Proteinsmentioning

confidence: 99%

Proteomic analysis of the sheep caruncular and intercaruncular endometrium reveals changes in functional proteins crucial for the establishment of pregnancy

Al-Gubory¹,

Arianmanesh²,

Garrel³

et al. 2014

Reproduction

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The expression and regulation of endometrial proteins are crucial for conceptus implantation and development. However, little is known about site-specific proteome profiles of the mammalian endometrium during the peri-implantation period. We utilised a two-dimensional gel electrophoresis/mass spectrometry-based proteomics approach to compare and identify differentially expressed proteins in sheep endometrium. Caruncular and intercaruncular endometrium were collected on days 12 (C12) and 16 (C16) of the oestrous cycle and at three stages of pregnancy corresponding to conceptus pre-attachment (P12), implantation (P16) and post-implantation (P20). Abundance and localisation changes in differentially expressed proteins were determined by western blot and immunohistochemistry. In caruncular endometrium, 45 protein spots (5% of total spots) altered between day 12 of pregnancy (P12) and P16 while 85 protein spots (10% of total spots) were differentially expressed between P16 and C16. In intercaruncular endometrium, 31 protein spots (2% of total spots) were different between P12 and P16 while 44 protein spots (4% of total spots) showed differential expression between C12 and C16. The pattern of protein changes between caruncle and intercaruncle sites was markedly different. Among the protein spots with implantation-related changes in volume, 11 proteins in the caruncular endometrium and six proteins in the intercaruncular endometrium, with different functions such as protein synthesis and degradation, antioxidant defence, cell structural integrity, adhesion and signal transduction, were identified. Our findings highlight the different but important roles of the caruncular and intercaruncular proteins during early pregnancy.

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“…It appears that the expression of VEGF is highly regulated in a temporal and spatial manner at the early stage of implantation (Demir et al 2010). In dairy goat, higher levels of VEGF mRNA were detected in the endometrium at D15 compared to D5, and VEGF-protein was expressed in the cytoplasm of LE, GE, and the SC.…”

Section: Discussionmentioning

confidence: 87%

The study of endometrium at gestational days 5 and 15 in dairy goats (Capra hircus)

Zhang¹,

Liu²,

Ping³

et al. 2017

Czech J. Anim. Sci.

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Zhang L., Liu X.-R., Han P., Zhou Z.-Q., Cao B.-Y., Song Y.-X. (2017): The study of endometrium at gestational days 5 and 15 in dairy goats (Capra hircus). Czech J. Anim. Sci., 62,[357][358][359][360][361][362][363][364][365][366][367][368] Endometrium receptivity, an absolutely necessary part of successful embryo implantation, and several morphological and biochemical endometrial receptivity biomarkers have already been studied and proposed in some animals. However, to our knowledge, no such a study has as yet been undertaken in dairy goat. In the present study, the serum and uterus of dairy goat were studied at gestational days 5 and 15, the estrogen (E2) and progesterone (P4) concentrations in serum were determined by electrochemiluminescence immunoassay, the surface topography of endometrium was observed by scanning electron microscopy. Furthermore, the estrogen receptors (ER), prolactin (PRL), some marker genes of receptive endometrium, and cell proliferation and apoptosis in the uterus were also detected. Well-formed pinopodes were found on the surface of endometrium at day 15 with higher E2 and P4 concentrations in the serum and higher estrogen receptors ERα and ERβ expression levels and a lower PRL level in the endometrium. Moreover, some expression levels of marker genes of receptive endometrium (OPN, VEGF, LIF, PRLR) were increased at day 15 compared to day 5, but no significant differences were observed in the cell proliferation and apoptosis in the uterus. The results showed that the endometrium reached the receptive state at gestational day 15 in dairy goats. Keywords: endometrial receptivity; pinopodes; gestational day 15 (D15)List of abbreviations: E2 = estrogen, P4 = progesterone, D = gestational day (D0, D1, D5, D15), ER = estrogen receptor, ERα = E2 receptor α, ERβ = E2 receptor β, PR = P4 receptor, PRL = prolactin, PRLR = prolactin receptor, PCNA = proliferating cell nuclear antigen, GHR = growth hormone receptor, IFG1R = insulin-like growth factor 1 receptor, OPN = osteopontin, VEGF = vascular endothelial growth factor, LIF = leukaemiainhibitory factor, RT-qPCR = quantitative real-time polymerase chain reaction, MPA = medroxyprogesterone acetate, FSH = follicle-stimulating hormone, PMSG = pregnant mare's serum gonadotropin, SEM = scanning electron microscopy, PA = paraformaldehyde, PBS = phosphate-buffered saline, ECLI = electrochemiluminescence immunoassay, HE = hematoxylin/eosin, IHC = immunohistochemistry, BSA = bull serum albumin, HRP = horseradish peroxidase, DAB = diaminobenzidine, NC = negative control, TUNEL = terminal deoxyribonucleotidyl transferase-mediated dUTP-digoxigenin nick end-labelling, LE = luminal epithelium, GE = glandular epithelium, SC = stromal cell, VE = vascular endothelial cell, IOD = integrated optical density, WOI = window of implantation, EEC = endometrial epithelial cells, ESC = endometrial stromal cells

show abstract

Vasculogenesis and angiogenesis in the endometrium during menstrual cycle and implantation

Cited by 168 publications

References 89 publications

WITHDRAWN: Endometrial epithelial cell apoptosis is inhibited via a ciR8073-miR181a-neurotensis pathway during embryo implantation

WITHDRAWN: Endometrial epithelial cell apoptosis is inhibited via a ciR8073-miR181a-neurotensis pathway during embryo implantation

Proteomic analysis of the sheep caruncular and intercaruncular endometrium reveals changes in functional proteins crucial for the establishment of pregnancy

The study of endometrium at gestational days 5 and 15 in dairy goats (Capra hircus)

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