Vasoactive intestinal peptide stimulation of human renal adenylate cyclase in vitro.

Abstract

3. Half-maximal stimulation of human renal cortical plasma membrane adenylate cyclase was observed with a mean value of 35 nM-VIP.4. The stimulation of renal adenylate cyclase by VIP appeared to be specific because stimulation by glucagon was additive to that obtained with VIP, and the VIP receptor antagonist (4 Cl-D-Phe6, Leul7)-VIP inhibited the VIP-dependent stimulation of adenylate cyclase activity.

A cultured human renal epithelioid cell line responsive to vasoactive intestinal peptide

“…The specific nature of the response is demonstrated by the comparative effects of related hormones and the anatgonistic actions of (4C1-D-Phe6,Leul7)-VIP, (Pandol, Dharmsathaphorn, Schoeffield, Vale & Rivier, 1986), (Ac-Tyrt,D-Phe2)-GRF (Waelbroecke, Robberecht, Coy, Camus, DeNeef & Christophe, 1985) and (10-28)VIP (Turner, Jones & Bylund, 1986) upon VIP stimulation of HEK (293) adenylate cyclase. Recently we have identified a VIP-stimulated adenylate cyclase in human renal cortical plasma membranes (Charlton, Neal & Simmons, 1989), though half-maximal activation is seen at 19 nM. In canine renal cortical plasma membranes 1251-VIP binding is characterized by both a high-affinity, low-capacity receptor (2)- (4) 43-3 +3-4…”

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“…In man and other mammals the patterns of segmentation are not distinct, with a gradual change in characteristics along the distal nephron; this is true for activation of adenylate cyclase by salmon calcitonin and PTH (Chabardes, Gagnan-Brunette, Imbert-Teboul, Gontcharevskaia, Montegut, Clique & Morel, 1980). VIP has been shown to activate adenylate cyclase in human renal plasma membranes, vi i a receptor present on distal tubular elements (Charlton, Neal & Simmons, 1990, 1992.…”

Renal epithelial Cl‐ secretion

“…In man and other mammals the patterns of segmentation are not distinct, with a gradual change in characteristics along the distal nephron; this is true for activation of adenylate cyclase by salmon calcitonin and PTH (Chabardes, Gagnan-Brunette, Imbert-Teboul, Gont-charevskaia, Montegut, Clique & Morel, 1980). VIP has been shown to activate adenylate cyclase in human renal plasma membranes, vi i a receptor present on distal tubular elements (Charlton, Neal & Simmons, 1990, 1992.…”