Vasoactive intestinal polypeptide and nitric oxide synthase distribution in the enteric plexuses of the human colon: an histochemical study and quantitative analysis

Matini, Patrizia; Faussone–Pellegrini, Maria Simonetta; Cortesini, C; Mayer, Bernd

doi:10.1007/bf01457541

Cited by 25 publications

(20 citation statements)

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“…Colocalization studies using different neuronal markers are crucial to determine subpopulations of neurons [10][11][12]. Distinct neuronal markers of subclasses of neurons in chagasic patients with megacolon have not been reported previously.…”

Section: Discussionmentioning

confidence: 96%

“…In this study, a double-labeling immunofluorescent technique using antibodies was employed to determine the population of neuronal bodies in the enteric plexuses of the colon from chagasic patients with megacolon. Calretinin has been used to identify intrinsic primary afferent neurons (IPANs), cChAT and SP to identify excitatory motor neurons, NPY to identify interneurons, and NOS and VIP to identify inhibitory motor neurons in the human colon [10,[12][13][14].…”

Section: Discussionmentioning

confidence: 99%

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Neurochemical Coding of the Enteric Nervous System in Chagasic Patients with Megacolon

et al. 2007

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Neuronal destruction has been considered the hallmark of pathogenic mechanisms in chagasic megacolon. Characterization of neuropeptides in the enteric nervous system from chagasic patients with megacolon could elucidate some aspects of the development of this syndrome. In the present work we demonstrate the changes in expression of neuropeptides and neurochemical markers present in neuronal plexuses from the colons of chagasic patients with megacolon. Sections of frozen tissue samples were immunohistochemically labeled for anticalretinin, cChaT, substance P, VIP, NOS, and NPY. Immunoreactivity was observed using a confocal microscope. Our results demonstrate that in chagasic patients with megacolon, inhibitory motor neurons (VIP and NOS immunoreactive) are preferentially destroyed by Trypanosoma cruzi and/or the inflammatory process. These results suggest a selective destruction of enteric neurons in the colon of chagasic patients with megacolon, pointing to an important discovery in the mechanism of pathogenesis of Chagas' disease.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Neurochemical Coding of the Enteric Nervous System in Chagasic Patients with Megacolon

et al. 2007

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“…NOS-IR myenteric plexus (MP) neurons were counted (twice by two different workers) in sections obtained from paraffin-embedded specimens which were first immunostained and further stained with haematoxylin-eosin (HE) to determine the MP neuron number per millimetre of section length (see Matini et al 1995) in rats sacrificed during the first week of postnatal life (P0, P3, P5, P7; two animals for each age group) and in adulthood (P30, P60, P90; two animals for each group of age). In order to ascertain whether the increase in NOS-immunoreactive neuron number occurred gradually or at a specific age, six 2-week-old rats and six 3-week-old rats were also studied.…”

Section: Morphometry and Statisticsmentioning

confidence: 99%

Neurochemical differentiation of rat enteric neurons during pre- and postnatal life

Matini

Mayer

Faussone–Pellegrini

1997

Cell and Tissue Research

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The presence of nitric oxide synthase (the enzyme which synthesizes nitric oxide), vasoactive intestinal peptide (VIP) and pituitary adenylyl-cyclase-activating peptide (PACAP) in the rat enteric nervous system between term (E18) and 90 days postpartum (P90) was investigated by immunohistochemistry. Neuronal maturity was ascertained by emanining for the presence of two novel neuron intermediate filament proteins: alpha-internexin, which is transiently expressed in developing neurons, and peripherin, which is expressed in differentiating as well as in mature neurons. The alimentary canal of the foetus at E18, unfed newborn, suckling, weaning and adult rats was studied. Throughout the rat gut the myenteric neurons were recognized by the time the foetal stage was reached and the submucous neurons by the suckling period. alpha-Internexin was present at E18; its levels increased during ageing and were markedly reduced at P30. Peripherin was first detected at birth; its levels increased with ageing and practically almost all of the neurons and nerve fibres were labelled from P30. Nitric oxide synthase was present at E18 and the number of labelled neurons gradually increased with ageing, while both VIP and PACAP could be detected by the end of the suckling period in an equal number of neurons to that in adulthood. These data indicate that in the rat gut the onset of the mature neuronal phenotype is not yet achieved at birth and that the neurochemical differentiation is accomplished during the first month of postnatal life.

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“…A constitutive nitric oxide synthase is present in neurons (NOS-1). In the gut it is localized in the neurons of enteric ganglia and in their processes (1)(2)(3). The function of NO produced by NOS-1 is relaxation of the smooth muscle.…”

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confidence: 99%

Changes in Distribution of Three Isoforms of Nitric Oxide Synthase in Ulcerative Colitis

Vento¹,

Kiviluoto²,

Järvinen³

et al. 2001

Scandinavian Journal of Gastroenterology

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Vasoactive intestinal polypeptide and nitric oxide synthase distribution in the enteric plexuses of the human colon: an histochemical study and quantitative analysis

Abstract: (a) the right colon contains a lower number of neurons and of VIP- and NOS-positive ones than the left colon, and (b) VIP- and NOS-positive fibers are differently distributed in the inner and outer portions of the circular muscle.

Cited by 25 publications

References 29 publications

Neurochemical Coding of the Enteric Nervous System in Chagasic Patients with Megacolon

Neurochemical Coding of the Enteric Nervous System in Chagasic Patients with Megacolon

Neurochemical differentiation of rat enteric neurons during pre- and postnatal life

Changes in Distribution of Three Isoforms of Nitric Oxide Synthase in Ulcerative Colitis

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