Vasopressin: Induced Structural Change in Toad Bladder Luminal Membrane

Kachadorian, W. A.; Wade, James B.; Discala, Vincent A.

doi:10.1126/science.809840

Cited by 210 publications

(96 citation statements)

References 24 publications

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“…The complementary e fracture face is shown in Fig. 9B; grooves can be seen in a pattern that matches the aggregates in the p face, as reported following vasopressin stimulation (Kachadorian, Wade & DiScala, 1975).…”

Section: Morphological Changes Induced By Nemmentioning

confidence: 53%

Activation of the vasopressin‐sensitive water permeability pathway in the toad bladder by N‐ethyl maleimide

Marples¹,

Bourguet²,

Taylor³

1994

Experimental Physiology

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SUMMARYVasopressin stimulates transepithelial water flow in the toad urinary bladder. We report here that N-ethyl maleimide (NEM) (0-1 mM) produces a similar increase in osmotic water flow when applied to the mucosal surface of the tissue. NEM-induced water flow is sensitive to inhibitors of hormone-induced water flow, including serosal acidification, or exposure to quinidine or cytoskeleton-disruptive drugs. NEM-induced water flow is additive with that induced by a submaximal, but not a maximal, dose of vasopressin. The response to mucosal NEM is not reversed on removal of the reagent, but established NEM-induced water flow can be inhibited by serosal acidification or quinidine. Like vasopressin, mucosal NEM induces the appearance of fusion profiles and intramembranous particle aggregates (putative water channels) in the apical plasma membrane of the granular cells, and the incidence of particle aggregates correlates with water flow. NEM does not cause an increase in intracellular cAMP.Our data suggest that NEM stimulates transepithelial water flow by irreversibly activating cellular mechanisms normally triggered by vasopressin, hence causing the insertion of water channels.

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Section: Morphological Changes Induced By Nemmentioning

confidence: 53%

Activation of the vasopressin‐sensitive water permeability pathway in the toad bladder by N‐ethyl maleimide

Marples¹,

Bourguet²,

Taylor³

1994

Experimental Physiology

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“…This alteration consists of the aggregation of intramembranous particles at multiple sites and is seen on the inner membrane fracture face. A similar observation in isolated toad urinary bladder after vasopressin stimulation has also been made and the specific localization of aggregates to luminal membranes of granular cells and the organization of aggregated particles in The Journal of Clinical Investigation Volume 59 March 1977*576-581 576 linear arrays have been established (8). Since for both preparations intramembranous particle aggregation occurs in the absence, as well as in the presence, of an osmotic gradient (9,10), the aggregation response can not be considered a consequence of net water flow per se, but rather appears to represent a primary hormonal effect.…”

Section: Introductionmentioning

confidence: 94%

“…Since for both preparations intramembranous particle aggregation occurs in the absence, as well as in the presence, of an osmotic gradient (9,10), the aggregation response can not be considered a consequence of net water flow per se, but rather appears to represent a primary hormonal effect. In addition, since the frequency of particle aggregation sites and the cumulative area of membrane occupied by them are linearly related to measured rates of vasopressin-induced osmotic water flow (8,10), it appears that these sites of aggregation may be of functional significance in the mechanism of action of vasopressin.…”

Section: Introductionmentioning

confidence: 99%

Relationship of aggregated intramembranous particles to water permeability in vasopressin-treated toad urinary bladder.

Kachadorian¹,

Levine²,

Wade³

et al. 1977

J. Clin. Invest.

106

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A B S T R A C T It has been previously demonstrated with freeze-fracture electron microscopy that vasopressin induces specific structural alterations of the luminal membrane of granular cells from toad urinary bladder in a dose-dependent fashion. These alterations consist of aggregated intramembranous particles and are observed both in the presence and absence of an osmotic gradient. We examined the effect of methohexital, a selective inhibitor of vasopressin-stimulated water flow, and the effect of phloretin, a selective inhibitor of urea permeability, on the structure of the granular cell luminal membrane. Methohexital treatment of the vasopressin-stimulated toad bladder reduced both the osmotic water flow and vasopressininduced alterations of membrane structure to the same extent. Phloretin reduced urea permeability but not water flow or particle aggregation. Since neither agent affects vasopressin-stimulated sodium movement, these findings indicate that the phenomenon of particle aggregation is specifically related to vaso-

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“…Such units (water channels or pores) are probably embedded in a structure revealed by the freeze-fracture technique as intramembrane particle (i.m.p.) aggregates (Chevalier, Bourguet & Hugon, 1974;Kachadorian, Wade & DiScala, 1975;Humbert, Montesano, Grosso, De Sousa & Orci, 1977;Wade, 1978). Similar aggregates were recently reported in toad skins exposed either to vasopressin or to isoprenaline (Brown, Grosso & De Sousa, 1979.…”

Section: -9+10-2mentioning

confidence: 99%

Osmotic water flow across the abdominal skin of the toad Bufo marinus: effect of vasopressin and isoprenaline

Sousa

Grosso

1982

The Journal of Physiology

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4. Methohexital and propranolol selectively inhibited the hydrosmotic effects of vasopressin and isoprenaline, respectively, whereas amiloride and ouabain had no effect.5. Mutual inhibition was found between vasopressin and isoprenaline in skins very sensitive to vasopressin. In less sensitive skins isoprenaline further increased Jw despite exposure of the epithelia to supramaximal concentrations of vasopressin. 6. Differential reactivity to vasopressin was found between the skin and the bladder taken from the same toad. In some instances, the bladder responded normally to vasopressin while the skin was totally unresponsive, suggesting the presence of osmoregulatory mechanisms exerting a local modulation of the vasopressin action in different target epithelia of the same animal.

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Vasopressin: Induced Structural Change in Toad Bladder Luminal Membrane

Cited by 210 publications

References 24 publications

Activation of the vasopressin‐sensitive water permeability pathway in the toad bladder by N‐ethyl maleimide

Activation of the vasopressin‐sensitive water permeability pathway in the toad bladder by N‐ethyl maleimide

Relationship of aggregated intramembranous particles to water permeability in vasopressin-treated toad urinary bladder.

Osmotic water flow across the abdominal skin of the toad Bufo marinus: effect of vasopressin and isoprenaline

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