Vasopressin stimulates long‐term net chloride secretion in cortical collecting duct cells

Abstract

The classical short-term effect (within minutes) of arginine vasopressin (AVP) consists in increasing sodium, chloride and water transport in kidney cells. More recently, long-term actions (several hours) of the hormone have been evidenced on water and sodium fluxes, due to transcriptional enhancement in the expression of their transporters. The present study demonstrates that AVP is also responsible for a long-term increase in net chloride secretion. In the RCCD 1 rat cortical collecting duct cell line, 10 38 M AVP induced, after several hours, an increase in net 36 Cl 3 secretion. This delayed effect of AVP was inhibited by basal addition of 10 34 M bumetanide and apical addition of 10 34 M glibenclamide, suggesting chloride entry at the basal membrane through a Na +

Calcyclin Is an Early Vasopressin-induced Gene in the Renal Collecting Duct

“…By contrast, very limited information is available on the long-term effects of this hormone. In addition to the long-term effects of AVP on water transport associated with an increase in AQP2 synthesis (12,26), we have recently shown that AVP exerts a delayed stimulation of sodium and chloride transport in collecting duct cells (13,14). This effect depends on transcription of several transporters of sodium and chloride, in particular ENaC, Na-KATPase, and CFTR.…”

“…This effect consists of a late increase in transepithelial water, sodium, and chloride transport after several hours. This late effect is mediated through a transcriptional/translational mechanism and involves an increase in the mRNA and de novo synthesis of different proteins such as AQP2; the ␤ and ␥ (but not ␣) subunits of ENaC (epithelial Na ϩ channel); the ␣ 1 (but not ␤ 1 ) subunit of Na ϩ /K ϩ -ATPase; and CFTR (cystic fibrosis transmembrane conductance regulator) (12)(13)(14)(15). This long-term effect may involve the genomic pathway by activation of cAMP-responsive elements in the promoter region of these genes and may ensure a sustained increase in sodium, chloride, and water transport in this segment of the nephron.…”

“…7B shows the quantification of the increase in AQP2 and calcyclin mRNA expression in four AVP-treated Brattleboro rats compared with that in four control Brattleboro rats. 1 Cells-Entry of an antibody inside the cell can be yielded by transient permeabilization procedures; thereafter, the cells recover and develop electrical properties similar to those of non-permeabilized cells (14,(21)(22)(23)(24)(25). To test the effect of anti-calcyclin antibodies on the AVP-induced increase in ion transport, two different cell permeabilization techniques were used to ensure antibody entry into the cells.…”

Cisplatin increases urinary sodium excretion in rats: gender-related differences

“…The classical short-term effect (within minutes) of vasopressin results in increased Na, Cl, and water transport in kidney cells. More recently, long-term actions (several hours) of vasopressin have been evidenced on water and Na fluxes, due to transcriptional enhancement in the expression of Na/K/2Cl-cotransporter (27). The V1 receptor mediates the natriuretic effect of vasopressin in rats (28).…”

The Cystic Fibrosis Transmembrane Regulator (CFTR) in the kidney

“…One possible role for CFTR in the kidney is the activation or inhibition of other channels like ORRC, ENaC and ROMK2 (Ismailov et al 1996, Wang 1999, Schwiebert et al 1995, Morris 1999). It has also been shown that argininevasopressin (AVP) produces an increase of chloride secretion due to transcriptional enhancement in the expression of CFTR and other transporters in rat cortical collecting ducts cells (Djelidi et al 1999).…”