Vasorelaxant effects and mechanisms of action of Heracleum sphondylium L. (Apiaceae) in rat thoracic aorta

Sénejoux, François; Demougeot, Céline; Cuciureanu, Magdalena; Miron, Anca M.; Cuciureanu, Rodica; Berthelot, Alain; Girard, Christian

doi:10.1016/j.jep.2013.03.030

Cited by 36 publications

(9 citation statements)

References 8 publications

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“…Sphondin and pimpinellin were found to be the most effective compounds, while psoralen showed the weakest activity (Dincel and others ). Senejoux and others () investigated the vasorelaxant effect of dichloromethane extract of H. sphondylium on rat isolated thoracic aortic rings. According to this study, the plant exhibited vasorelaxant properties through an endothelium‐independent mechanism involving the inhibition of Ca 2+ mobilization and changes in Kv channel conductance.…”

Section: Biological Activitiesmentioning

confidence: 99%

The Genus Heracleum: A Comprehensive Review on Its Phytochemistry, Pharmacology, and Ethnobotanical Values as a Useful Herb

Bahadori

Dinparast

Zengin

2016

Comp Rev Food Sci Food Safe

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Heracleum species, also known as hogweed, are traditionally used as food additives, spices, and flavoring agents. Moreover, these plants are widely used in folklore medicine for the treatment of many disorders such as inflammation, flatulence, stomachache, epilepsy, psoriasis, and as carminative, wound healing, antiseptic, antidiarrheal, tonic, digestive, pain killer, analgesic, and anticonvulsant agents. The genus Heracleum has broad pharmacological activities: anti-inflammatory, antimicrobial, anticholinesterase, anti-oxidant, antiviral, cytotoxic, and anticarcinogenic. A total of 94 compounds have been isolated from plants of the Heracleum genus, all indicating vital biological activities. Also, about 50 compounds have been identified as major components in their essential oils. The genus is rich in several types of bioactive coumarin compounds, with huge potential for the discovery of new coumarins. Various parts of these plants produce essential oils (mainly aliphatic esters and monoterpenes) with a wide spectrum of biological activities. Heracleum species have great potential for applications in the food, cosmetics, perfumery, and pharmaceutical industries due to their broad ethnobotanical uses and pharmacological properties. Accordingly, this review aims to categorize updated and comprehensive information on ethnobotanical uses, phytochemistry, and pharmacology of Heracleum species in order to open new perspectives for future studies, including possible uses as functional ingredients in food products.

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Section: Biological Activitiesmentioning

confidence: 99%

The Genus Heracleum: A Comprehensive Review on Its Phytochemistry, Pharmacology, and Ethnobotanical Values as a Useful Herb

Bahadori

Dinparast

Zengin

2016

Comp Rev Food Sci Food Safe

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“…Two stainless steel triangles were passed through the lumen of each ring. One triangle was connected to an isometric force transducer connected to a BIOPAC polygraph (MP100WSW, Biopac Systems, Inc, Goleta, CA, USA) to measure tension in the vessels [16,17] . The rings were mounted in organ baths containing 10 mL Krebs-Henseleit (K-H) solution of the following composition [18] .…”

Section: Rho Kinase Activity Assaymentioning

confidence: 99%

DL0805-2, a novel indazole derivative, relaxes angiotensin II-induced contractions of rat aortic rings by inhibiting Rho kinase and calcium fluxes

et al. 2016

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Aim: DL0805-2 [N-(1H-indazol-5-yl)-1-(4-methylbenzyl) pyrrolidine-3-carboxamide] is a DL0805 derivative with more potent vasorelaxant activity and lower toxicity. This study was conducted to investigate the vasorelaxant mechanisms of DL0805-2 on angiotensin II (Ang II)-induced contractions of rat thoracic aortic rings in vitro. Methods: Rat thoracic aortic rings and rat aortic vascular smooth muscle cells (VSMCs) were pretreated with DL0805-2, and then stimulated with Ang II. The tension of the aortic rings was measured through an isometric force transducer. Ang II-induced protein phosphorylation, ROS production and F-actin formation were assessed with Western blotting and immunofluorescence assays. Intracellular free Ca 2+ concentrations were detected with Fluo-3 AM. Results: Pretreatment with DL0805-2 (1-100 μmol/L) dose-dependently inhibited the constrictions of the aortic rings induced by a single dose of Ang II (10 -7 mol/L) or accumulative addition of Ang II (10 -10 -10 -7 mol/L). The vasodilatory effect of DL0805-2 was independent of endothelium. In the aortic rings, pretreatment with DL0805-2 (1, 3, and 10 μmol/L) suppressed Ang II-induced Ca 2+ influx and intracellular Ca 2+ mobilization, and Ang II-induced phosphorylation of two substrates of Rho kinase (MLC and MYPT1). In VSMCs, pretreatment with DL0805-2 (1, 3, and 10 μmol/L) also suppressed Ang II-induced Ca 2+ fluxes and phosphorylation of MLC and MYPT1. In addition, pretreatment with DL0805-2 attenuated ROS production and F-actin formation in the cells. Conclusion: DL0805-2 exerts a vasodilatory action in rat aortic rings through inhibiting the Rho/ROCK pathway and calcium fluxes.

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“…Ca 2+ was added to the organ bath cumulatively at the concentration (10 -4 →x 10 -1 mM) to evoke dose-response contraction curve after which the tissues were wash and allowed to stabilize in K + rich and Ca 2+ free Krebs's solution. The protocol was repeated on the aortic rings incubated with 0.5, 1 and 2 mg/mL of NBF-ASME fraction for 15 minute before contraction with PE and cumulative addition of Ca 2+ (10 -4 →10 -2 M) (Senejoux et al, 2013).…”

Section: Effect Of Nbf-asme On Extracellular Ca 2+ Influxmentioning

confidence: 99%

“…After, equilibration, aortic rings were pre-incubated with 0.5 1 and 2 mg/mL EC 50 concentration of NBF-ASME for 15 minute before stimulating contraction with PE (1.0 µM) to induce the second transient contraction. The ratio between the first and second transient contractions was calculated (T 1 /T 2 ) as described (Dimo et al, 2007;Senejoux et al, 2013).…”

Section: Effect Of Nbf-asme On Intracellular Ca 2+ Releasementioning

confidence: 99%

Mechanisms underlying the antihypertensive effect of Alstonia scholaris

Bello

Usman

Mahmud

et al. 2015

Journal of Ethnopharmacology

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Vasorelaxant effects and mechanisms of action of Heracleum sphondylium L. (Apiaceae) in rat thoracic aorta

Cited by 36 publications

References 8 publications

The Genus Heracleum: A Comprehensive Review on Its Phytochemistry, Pharmacology, and Ethnobotanical Values as a Useful Herb

The Genus Heracleum: A Comprehensive Review on Its Phytochemistry, Pharmacology, and Ethnobotanical Values as a Useful Herb

DL0805-2, a novel indazole derivative, relaxes angiotensin II-induced contractions of rat aortic rings by inhibiting Rho kinase and calcium fluxes

Mechanisms underlying the antihypertensive effect of Alstonia scholaris

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