VCAM1-targeted RNA interference inhibits the proliferation of human oral squamous carcinoma HN12 cells

Abstract

In the present study, RNA interference (RNAi) was used to investigate the effect of vascular cell adhesion molecule 1 (VCAM1) silencing on the proliferation of human oral squamous carcinoma HN12 cells. HN12 cells were divided into three groups: The untreated blank control cell group (CK), the negative control group transfected with non-homologous vector (NC) and the positive group transfected with the target sequence VCAM1 small hairpin RNA (KD). Reverse-transcription polymerase chain reaction and western blot analysis were used to examine the effects of VCAM1-knockdown on the mRNA expression of VCAM1 and subsequent protein expression. Furthermore, the HN12 cell growth inhibition rate was detected using the cell counting kit-8 method. The VCAM1-targeted lentiviral vector RNAi significantly inhibited VCAM1 mRNA, and subsequent protein, expression. Compared with the NC group, the VCAM1 gene knockdown efficiency was ~85%, while the expression level of VCAM1 protein was reduced by ~74% in KD group cells. In addition, cell growth was significantly inhibited in the KD group, with a growth inhibition rate of ~34%. Therefore, this targeted lentiviral vector RNAi effectively inhibited VCAM1 gene, and subsequent protein, expression, as well as the proliferation of oral squamous carcinoma cells. These results may provide an experimental reference for the diagnosis and treatment of oral squamous cell carcinoma.

An integrated characterization of contractile, electrophysiological, and structural cardiotoxicity of Sophora tonkinensis Gapnep. in human pluripotent stem cell-derived cardiomyocytes

“…The cell viability was evaluated using Cell Counting Kit 8 (CCK-8) and trypan blue exclusion assay [20], and hiPSC-CMs were incubated with different concentrations of matrine, oxymatrine, cytisine, and sophocarpine (2, 10, 50 μM) for 24 h (Additional file 1). The results showed cell viability decreased dose-dependently with increasing dose concentrations, particularly cell viability was significantly decreased at the 10 μM dose in Fig.…”

Integrated network pharmacology and experimental verification to explore the potential mechanism of San Ying decoction for treating triple-negative breast cancer

“…Studies have shown that overexpression of VCAM1 can enhance tumor invasion and migration and promote tumor metastasis [22]. Suppressing the expression of VCAM1 can reduce the proliferation ability of tumors [23]. The percentage of patients with positive VCAM1 expression in breast cancer tissues is increased, and the expression rate is greater in patients with lymph San Ying decoction for treating triple-negative breast cancer 773 node metastasis.…”

Influence of Aerosolization on Endothelial Cells for Efficient Cell Deposition in Biohybrid and Regenerative Applications

“…An interesting aspect to further investigate would be the differences in the protein expression of sprayed cells. It has been shown that inhibited gene expression of VCAM-1 correlates with inhibited protein expression [ 46 ]. Interestingly, MCP-1 protein expression levels do not directly correspond to mRNA expression [ 47 ].…”