2002
DOI: 10.1091/mbc.01-07-0368
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VE-Cadherin Regulates Endothelial Actin Activating Rac and Increasing Membrane Association of Tiam

Abstract: Previously published reports support the concept that, besides promoting homotypic intercellular adhesion, cadherins may transfer intracellular signals. However, the signaling pathways triggered by cadherin clustering and their biological significance are still poorly understood. We report herein that transfection of VE-cadherin (VEC) cDNA in VEC null endothelial cells induces actin rearrangement and increases the number of vinculin positive adhesion plaques. VEC expression augments the level of active Rac but… Show more

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Cited by 231 publications
(243 citation statements)
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“…Lampugnani and colleagues studied the effect of VE-cadherin expression on Rho-GTPase activity. 88 Compared with VE-cadherin-deficient endothelial cells, VE-cadherin-reconstituted cells showed increased Rac1 activity and decreased activation of RhoA. This increase in Rac1 activity was suggested to be mediated by the GEF Tiam-1, since expression of VE-cadherin increased protein and mRNA levels of Tiam-1 and induced its localization at cell-cell junctions.…”
Section: Endothelial Adherens Junction Control By Rho Gtpasesmentioning
confidence: 97%
See 1 more Smart Citation
“…Lampugnani and colleagues studied the effect of VE-cadherin expression on Rho-GTPase activity. 88 Compared with VE-cadherin-deficient endothelial cells, VE-cadherin-reconstituted cells showed increased Rac1 activity and decreased activation of RhoA. This increase in Rac1 activity was suggested to be mediated by the GEF Tiam-1, since expression of VE-cadherin increased protein and mRNA levels of Tiam-1 and induced its localization at cell-cell junctions.…”
Section: Endothelial Adherens Junction Control By Rho Gtpasesmentioning
confidence: 97%
“…This increase in Rac1 activity was suggested to be mediated by the GEF Tiam-1, since expression of VE-cadherin increased protein and mRNA levels of Tiam-1 and induced its localization at cell-cell junctions. 88 Others showed that endothelial junction disruption in response to TNF-a was mediated by the GEF P-Rex1 through activation of Rac1. The P-Rex1-Rac1 signaling axis was described to induce generation of reactive oxygen species (ROS).…”
Section: Endothelial Adherens Junction Control By Rho Gtpasesmentioning
confidence: 99%
“…Briefly, the cDNA fragment encoding RhoA N19 was mutagenized from pEGFP-WT-RhoA (gift from M. Philips, New York University, New York, NY). The cDNA fragment encoding VE⌬ (Navarro et al, 1995;Lampugnani et al, 2002) was polymerase chain reaction amplified from pBluescript-VE-cadherin-EGFP (clone 35-21; gift from S. Shaw and F. Luscinskas, Brigham and Women's Hospital, Boston, MA) by using the following primers: 5Ј-GAGGCGGCCGCCCACCATGCA-GAGGCTCATGATG-3Ј and 5Ј-GAGCTCGAGCTAATACAGCAGCTCCTC-CCGGGGG-3Ј. Both fragments were polymerase chain reaction amplified and cloned into the shuttle vector pShuttle-IRES-hrGFP-1.…”
Section: Construction Of Recombinant Adenovirusesmentioning
confidence: 99%
“…Cadherin-mediated adhesion also directly initiates both mechanical and biochemical signals known to affect FA formation. First, mechanical stress exerted at cadherin cell Carmeliet et al, 1999;Etienne-Manneville and Hall, 2002;Juliano, 2002;Lampugnani et al, 2002;Kouklis et al, 2003). Yet, despite the logical existence and biological ramifications of cross talk between these adhesion systems, previous attempts to demonstrate specifically the effects of cell-cell contact on cell-ECM adhesion have remained controversial.…”
Section: Introductionmentioning
confidence: 99%
“…Modulation of cell-cell contacts that regulate cell adhesion and cell motility likely requires interactions between cadherins and catenins, and it has been shown that p120 catenin regulates actin cytoskeletal organization and cell motility by activation of Rho GTPases [269][270][271]. In addition, VEcadherin associates with VEGFR-2, known to be transactivated by the P2Y 2 R [20,89], and also with Src, Shc, Csk [272,273], and the vascular endothelial protein tyrosine phosphatase, VE-PTP [274]. VEGFR-2 activation in endothelial cells has been shown to stimulate the tyrosine phosphorylation of VE-cadherin, β-and p120-catenins, plakoglobin, and PECAM-1 [254].…”
Section: Proinflammatory P2y 2 R Functions In Endotheliummentioning
confidence: 99%