Vector correlation technique for pixel-wise detection of collagen fiber realignment during injurious tensile loading

Quinn, Kyle P.; Winkelstein, Beth A.

doi:10.1117/1.3227037

Cited by 35 publications

(118 citation statements)

References 36 publications

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“…70,72,[74][75][76] In particular, those studies collectively demonstrate that areas with the highest fiber realignment correspond to those regions with microstructural damage to the collagen matrix (FIGURE 3B). These studies have shown that areas of greatest fiber realignment are significantly correlated with subsequent rupture, 70,72,76 but do not necessarily correspond to areas of peak maximum principal or shear strain. 72,76 Together, these findings suggest that collagen realignment may be a more sensitive indicator of microstructural injury than strain measurements, and further highlight the possibility that afferent fibers may be more susceptible to activation if they reside in regions where the collagenous matrix undergoes abnormal deformation.…”

Section: Kinematics and Facet Tissue Injury During Whiplashmentioning

confidence: 94%

“…These studies have shown that areas of greatest fiber realignment are significantly correlated with subsequent rupture, 70,72,76 but do not necessarily correspond to areas of peak maximum principal or shear strain. 72,76 Together, these findings suggest that collagen realignment may be a more sensitive indicator of microstructural injury than strain measurements, and further highlight the possibility that afferent fibers may be more susceptible to activation if they reside in regions where the collagenous matrix undergoes abnormal deformation. Nevertheless, fiber realignment is correlated with areas in the ligament in which there is unrecovered strain after whiplash-like loading.…”

Section: Kinematics and Facet Tissue Injury During Whiplashmentioning

confidence: 99%

“…Quantitative polarized light imaging is an optical technique that exploits the natural birefringent optical property of collagen molecules to quantify the dynamic reorganization of collagen fibers during mechanical loading, and has been used extensively to evaluate soft tissue biomechanics and other collagenous tissue equivalents. 49,76,79,98 Using this approach, collagen fiber realignment during tensile and posterior retraction loading of isolated human cadaveric facets, and tensile loading of isolated rat facets, has been defined. 70,72,[74][75][76] In particular, those studies collectively demonstrate that areas with the highest fiber realignment correspond to those regions with microstructural damage to the collagen matrix (FIGURE 3B).…”

Section: Kinematics and Facet Tissue Injury During Whiplashmentioning

confidence: 99%

“…49,76,79,98 Using this approach, collagen fiber realignment during tensile and posterior retraction loading of isolated human cadaveric facets, and tensile loading of isolated rat facets, has been defined. 70,72,[74][75][76] In particular, those studies collectively demonstrate that areas with the highest fiber realignment correspond to those regions with microstructural damage to the collagen matrix (FIGURE 3B). These studies have shown that areas of greatest fiber realignment are significantly correlated with subsequent rupture, 70,72,76 but do not necessarily correspond to areas of peak maximum principal or shear strain.…”

Section: Kinematics and Facet Tissue Injury During Whiplashmentioning

confidence: 99%

“…43 However, as described above, subfailure stretch of the facet capsule exceeding physiologic strains is sufficient to damage the ligament's microstructure and induce pain. 27,76 As such, characterizing the functional changes of neurons and understanding their relationships to the local injury mechanisms during loading below the tissue failure threshold are important, and many studies have initiated these investigations using both in vivo and in vitro models.…”

Section: Neuronal Responses To Excessive Stretchmentioning

confidence: 99%

See 4 more Smart Citations

The Physiological Basis of Cervical Facet-Mediated Persistent Pain: Basic Science and Clinical Challenges

Ita¹,

Zhang²,

Holsgrove³

et al. 2017

Journal of Orthopaedic & Sports Physical Therapy

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FIGURE 1. Schematic illustrating the anatomy in the periphery of the facet joint and the relevant neuronal connections to the central nervous system. Afferents that innervate the facet joint and its capsular ligament have cell bodies in the DRG and synapse with neurons in the spinal dorsal horn. Nociceptive information is encoded by many types of afferents, including IB4-positive nonpeptidergic neurons and peptidergic fibers that produce neuropeptides, such as CGRP and substance P. Noxious stimuli are translated into electrical (eg, action potentials) and biochemical (eg, neurotransmitter) signals. In persistent pain, central sensitization occurs, with neuronal hyperexcitability and altered neurotransmitter production and release in the spinal dorsal horn. Abbreviations: CGRP, calcitonin gene-related peptide; DRG, dorsal root ganglion; IB4, isolectin B4.

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Section: Kinematics and Facet Tissue Injury During Whiplashmentioning

confidence: 94%

Section: Kinematics and Facet Tissue Injury During Whiplashmentioning

confidence: 99%

Section: Kinematics and Facet Tissue Injury During Whiplashmentioning

confidence: 99%

Section: Kinematics and Facet Tissue Injury During Whiplashmentioning

confidence: 99%

Section: Neuronal Responses To Excessive Stretchmentioning

confidence: 99%

See 3 more Smart Citations

The Physiological Basis of Cervical Facet-Mediated Persistent Pain: Basic Science and Clinical Challenges

Ita¹,

Zhang²,

Holsgrove³

et al. 2017

Journal of Orthopaedic & Sports Physical Therapy

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Collagen organization regulates stretch‐initiated pain‐related neuronal signals in vitro: Implications for structure–function relationships in innervated ligaments

Zhang

Singh

Winkelstein

2017

Journal Orthopaedic Research

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Injury to the spinal facet capsule, an innervated ligament with heterogeneous collagen organization, produces pain. Although mechanical facet joint trauma activates embedded afferents, it is unclear if, and how, the varied extracellular microstructure of its ligament affects sensory transduction for pain from mechanical inputs. To investigate the effects of macroscopic deformations on afferents in collagen matrices with different organizations, an in vitro neuron-collagen construct (NCC) model was used. NCCs with either randomly organized or parallel aligned collagen fibers were used to mimic the varied microstructure in the facet capsular ligament. Embryonic rat dorsal root ganglia (DRG) were encapsulated in the NCCs; axonal outgrowth was uniform and in all directions in random NCCs, but parallel in aligned NCCs. NCCs underwent uniaxial stretch (0.25 ± 0.06 strain) corresponding to sub-failure facet capsule strains that induce pain. Macroscopic NCC mechanics were measured and axonal expression of phosphorylated extracellular signal-regulated kinase (pERK) and the neurotransmitter substance P (SP) was assayed at 1 day to assess neuronal activation and nociception. Stretch significantly upregulated pERK expression in both random and aligned gels (p < 0.001), with the increase in pERK being significantly higher (p = 0.013) in aligned than in random NCCs. That increase likely relates to the higher peak force (p = 0.025) and stronger axon alignment (p < 0.001) with stretch direction in the aligned NCCs. In contrast, SP expression was greater in stretched NCCs (p < 0.001) regardless of collagen organization. These findings suggest that collagen organization differentially modulates pain-related neuronal signaling and support structural heterogeneity of ligament tissue as mediating sensory function. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:770-777, 2018.

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Cervical facet capsular ligament mechanics: Estimations based on subject‐specific anatomy and kinematics

et al. 2023

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BackgroundTo understand the facet capsular ligament's (FCL) role in cervical spine mechanics, the interactions between the FCL and other spinal components must be examined. One approach is to develop a subject‐specific finite element (FE) model of the lower cervical spine, simulating the motion segments and their components' behaviors under physiological loading conditions. This approach can be particularly attractive when a patient's anatomical and kinematic data are available.MethodsWe developed and demonstrated methodology to create 3D subject‐specific models of the lower cervical spine, with a focus on facet capsular ligament biomechanics. Displacement‐controlled boundary conditions were applied to the vertebrae using kinematics extracted from biplane videoradiography during planar head motions, including axial rotation, lateral bending, and flexion–extension. The FCL geometries were generated by fitting a surface over the estimated ligament–bone attachment regions. The fiber structure and material characteristics of the ligament tissue were extracted from available human cervical FCL data. The method was demonstrated by application to the cervical geometry and kinematics of a healthy 23‐year‐old female subject.ResultsFCL strain within the resulting subject‐specific model were subsequently compared to models with generic: (1) geometry, (2) kinematics, and (3) material properties to assess the effect of model specificity. Asymmetry in both the kinematics and the anatomy led to asymmetry in strain fields, highlighting the importance of patient‐specific models. We also found that the calculated strain field was largely independent of constitutive model and driven by vertebrae morphology and motion, but the stress field showed more constitutive‐equation‐dependence, as would be expected given the highly constrained motion of cervical FCLs.ConclusionsThe current study provides a methodology to create a subject‐specific model of the cervical spine that can be used to investigate various clinical questions by coupling experimental kinematics with multiscale computational models.

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Vector correlation technique for pixel-wise detection of collagen fiber realignment during injurious tensile loading

Cited by 35 publications

References 36 publications

The Physiological Basis of Cervical Facet-Mediated Persistent Pain: Basic Science and Clinical Challenges

The Physiological Basis of Cervical Facet-Mediated Persistent Pain: Basic Science and Clinical Challenges

Collagen organization regulates stretch‐initiated pain‐related neuronal signals in vitro: Implications for structure–function relationships in innervated ligaments

Cervical facet capsular ligament mechanics: Estimations based on subject‐specific anatomy and kinematics

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