Paleofloristic reconstruction based on the palynological assemblages recovered from lacustrine Villa Alegria and Cerro Prieto of the Tehuacan-Cuicatlan Valley, Puebla andOaxaca.The main objective of this research was to reconstruct the paleoflora of Lacustrine Villa Alegria (named by Dávalos-Álvarez, 2006) and the Cerro Prieto travertine through paleopalynological analysis, in addition to establishing palynofloristic affinities with other Neogene and Paleogene formations found in Mexico. The study area includes two localities situated within the Tehuacan-Cuicatlan Valley: Lacustrine Villa Alegria to the north of the valley in Puebla State, and the Cerro Prieto travertine in the south of the valley, Oaxaca State. In Lacustrine Villa Alegria, three sections were prospected and a total of 45 travertine rock samples were collected. In the Cerro Prieto travertine, five sections were prospected and a total of sixty samples were collected. All samples were processed under the conventional method of extracting fossil pollen (Traverse, 2007). In Lacustrine Villa Alegria, 44 samples contained a total of 18,389 palynomorphs was counted, of which 2,240 pertain to pollen and spores, while the rest were algae and fungal spores. 68 taxa were identified, of which 80% (n = 55) match angiosperms, 5.9% (n = 4) match gymnosperms and 14.7% (n = 10) are spores. In the Cerro Prieto travertine, of the 60 processed samples 58 were positive. Sums of 14,935 palynomorphs were counted there, of which 2546 pertain to pollen and spores and 12,412 are algae and fungal spores. 53 taxa were identified, of which 71.7% (n = 38) match angiosperms, 7.5% (n = 4) are gymnosperms and 20.7% (n = 11) pertain to spores.The paleopalynological assemblages recovered from both locations were plotted with the Tilia Graph (Grimm, 1997) program. They were analyzed using the CONISS method in order to determine palynozones. In sections VA-2 and VA-3 of Lacustrine Villa Alegria, the most abundant floristic element was Fraxinus sp., followed by Alnus sp. and Asteraceae. In section VA-1 the dominant element was Bursera sp., followed by Anacardiaceae. In most sections of the Cerro Prieto travertine, the genera that dominated palynological assemblages were Fraxinus sp, Quercus sp. and Pinus sp. Bursera sp. was dominant in section CP-3 of said locality. Two palynozones were defined in all Lacustrine Villa Alegria and Cerro Prieto travertine sections; they were characterized by different taxa, and the greatest diversity of species was found to be concentrated mid-section. Richness (S), diversity (H') and evenness (J') of the taxa recovered within the palynological assemblages were analyzed.In Lacustrine Villa Alegria, the highest level of richness was obtained in section VA-3 with S = 2 and the highest Shannon Wiener diversity rate was obtained in section VA-2 with H'= 2.95, as well as the highest value of the Pielou evenness index with J '= 0.77. For the Cerro Prieto travertine, the highest richness value was obtained from section 3 with S = 38, the highest diversity was...