Vegetative Cell and Spore Proteomes of<i>Clostridioides difficile</i>show finite differences and reveal potential biomarkers.

Abhyankar, Wishwas; Zheng, Lirong; Brul, Stanley; Koster, Chris G. de; Koning, Leo J. de

doi:10.1101/598045

Cited by 1 publication

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“…The B. cereus homolog with the best match to B. subtilis YetF (UniProt ID Q813Q8), as well as a 2Duf homolog (UniProt ID Q812J9), were also present in the B. cereus spore IM proteome (iBAQ ranks 559 and 626, respectively out of 1,254), but not in vegetative cells or the vegetative cell membrane fraction (Gao et al, 2021). In addition, the one C. difficile spores' 219 aa YetF homolog (UniProt ID Q187A0) was also identified in these spores' IM proteome, with an approximate abundance at the median out of 1,021 proteins identified; this protein was also absent from the vegetative cell membrane fraction (Abhyankar et al, 2019).…”

Section: Presence and Relative Levels Of Yetf Homologs In Growing Cel...mentioning

confidence: 98%

“…There are many publications reporting mass spectrometry analyses of the proteome of B. subtilis, B. cereus and Clostridiodes difficile vegetative cells and spores, including IM proteins (Zheng et al, 2016;Swarge et al, 2018;Abhyankar et al, 2019;Swarge et al, 2020;Gao et al, 2021Gao et al, , 2022. Such work identified all five YetF homologs in the B. subtilis spore IM (Zheng et al, 2016).…”

Section: Presence and Relative Levels Of Yetf Homologs In Growing Cel...mentioning

confidence: 99%

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Identification and characterization of new proteins crucial for bacterial spore resistance and germination

Kanaan

Shames

et al. 2023

Front. Microbiol.

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2Duf, named after the presence of a transmembrane (TM) Duf421 domain and a small Duf1657 domain in its sequence, is likely located in the inner membrane (IM) of spores in some Bacillus species carrying a transposon with an operon termed spoVA2mob. These spores are known for their extreme resistance to wet heat, and 2Duf is believed to be the primary contributor to this trait. In this study, we found that the absence of YetF or YdfS, both Duf421 domain-containing proteins and found only in wild-type (wt) B. subtilis spores with YetF more abundant, leads to decreased resistance to wet heat and agents that can damage spore core components. The IM phospholipid compositions and core water and calcium-dipicolinic acid levels of YetF-deficient spores are similar to those of wt spores, but the deficiency could be restored by ectopic insertion of yetF, and overexpression of YetF increased wt spore resistance to wet heat. In addition, yetF and ydfS spores have decreased germination rates as individuals and populations with germinant receptor-dependent germinants and increased sensitivity to wet heat during germination, potentially due to damage to IM proteins. These data are consistent with a model in which YetF, YdfS and their homologs modify IM structure to reduce IM permeability and stabilize IM proteins against wet heat damage. Multiple yetF homologs are also present in other spore forming Bacilli and Clostridia, and even some asporogenous Firmicutes, but fewer in asporogenous species. The crystal structure of a YetF tetramer lacking the TM helices has been reported and features two distinct globular subdomains in each monomer. Sequence alignment and structure prediction suggest this fold is likely shared by other Duf421-containing proteins, including 2Duf. We have also identified naturally occurring 2duf homologs in some Bacilli and Clostridia species and in wt Bacillus cereus spores, but not in wt B. subtilis. Notably, the genomic organization around the 2duf gene in most of these species is similar to that in spoVA2mob, suggesting that one of these species was the source of the genes on this operon in the extremely wet heat resistant spore formers.

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Section: Presence and Relative Levels Of Yetf Homologs In Growing Cel...mentioning

confidence: 98%

Section: Presence and Relative Levels Of Yetf Homologs In Growing Cel...mentioning

confidence: 99%

Identification and characterization of new proteins crucial for bacterial spore resistance and germination

Kanaan

Shames

et al. 2023

Front. Microbiol.

Self Cite

View full text Add to dashboard Cite

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Vegetative Cell and Spore Proteomes ofClostridioides difficileshow finite differences and reveal potential biomarkers.

Abstract: 1 Clostridioides difficile show finite differences and 2 reveal potential protein markers. Abstract 1 7

Cited by 1 publication

References 74 publications

Identification and characterization of new proteins crucial for bacterial spore resistance and germination

Identification and characterization of new proteins crucial for bacterial spore resistance and germination

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