Vegetative compatibility groups of Japanese isolates of Verticillium dahliae

Wakatabe, Dai; Nagao, Hideyuki; Arai, Hiroaki; Shiraishi, Toshimasa; Koike, Masanori; Iijima, Tsutomu

doi:10.1007/bf02464964

Cited by 17 publications

(38 citation statements)

References 19 publications

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“…Each pair of isolates represented the subgroup provisionally designated as J1, J2, and J3 depending upon their compatible reactions (Wakatabe et al, 1997). Recovery and characterization of nit mutants Nit mutants were generated according to a modified method of Puhalla (1985).…”

Section: Methodsmentioning

confidence: 99%

“…Recovery and characterization of nit mutants Nit mutants were generated according to a modified method of Puhalla (1985). Procedures for nit mutants were conducted as previously reported (Wakatabe et al, 1997). Complementation tests Pairings were conducted by placing two mycelial blocks of nit1 and/or NitM mutants 15 mm apart on MM in 90-ram Petri dishes.…”

Section: Methodsmentioning

confidence: 99%

“…Isolates of pepper patho-*E-maih sakazuki@midori.h, chiba-u.ac.jp. type did not show pathogenicity to tomato in the artificial inoculation (Wakatabe et al, 1997). Nor did tomato pathotype show pathogenicity to pepper in the artificial inoculation Wakatabe et al, 1997).…”

mentioning

confidence: 92%

“…type did not show pathogenicity to tomato in the artificial inoculation (Wakatabe et al, 1997). Nor did tomato pathotype show pathogenicity to pepper in the artificial inoculation Wakatabe et al, 1997). In 1991, four isolates of V. dahliae were obtained from diseased peppers in a field in Nagano Pref.…”

mentioning

confidence: 99%

“…Verticillium dahliae was genetically classified into four VC groups using nitrate nonutilizing (nit) mutants Rowe, 1990, 1991;Strausbaugh et al, 1992;Strausbaugh, 1993). Japanese isolates of V. dahliae comprised a VCG including three subgroups (J1, J2 and J3) distinguished by the strength of heterokaryon (Wakatabe et al, 1997). There was a good relationship between pathogenicity to tomato and vegetative compatibility.…”

mentioning

confidence: 99%

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Vegetative compatibility of an isolate of Verticillium dahliae pathogenic to both tomato and pepper

Nagao

Arai

Oshima³

et al. 1998

Mycoscience

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An isolate of Verticillium dahliae Vdp-4, pathogenic to both tomato and pepper (tomato-pepper pathotype), was examined for its vegetative compatibility with testers of the Japanese vegetative compatibility group (subgroups J 1, J2, and J3). Seven isolates of V. dahliae from the same field as Vdp-4 in Misato, Nagano Pref. and two isolates from Hokkaido were separately determined as either tomato pathotype (B) or pepper pathotype (C). Isolate 5922 previously reported as tomato-pepper pathotype was also examined. Compatible nit1 and NitM mutants were obtained from all isolates except for isolates Vdp-3 and Vdt-lO. The isolate of tomato-pepper pathotype Vdp-4 showed a strong reaction with VCGJ 1 and J3 and was thus assigned to J3. Seven of these isolates showed compatibility and were assigned into three provisional subgroups. The isolate 5922 was self-incompatible.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 92%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Vegetative compatibility of an isolate of Verticillium dahliae pathogenic to both tomato and pepper

Nagao

Arai

Oshima³

et al. 1998

Mycoscience

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Assessment of vegetative compatibility of race-2 tomato wilt isolates of Verticillium dahliae in Japan

Nagao

Shiraishi²,

Oshima³

et al. 1997

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Verticillium dahliae race-2 can invade the resistant cultivars of tomato possessing the Ve gene. This new race was recently found in several regions in Japan, and 10 isolates of V. dahliae race-2 from these regions were used in our study. Pathogenicity tests identified these isolates as the tomato pathotype (B). We examined the vegetative compatibility of 8 of these 10 Japanese isolates of V. dahliae race-2 to estimate their genetic relatedness with the testers of Japanese vegetative compatibility group previously proposed (VCGJ) using nit mutants. Compatible nit1 and NitM mutants were obtained from all V. dahliae race-2 isolates. Selected representative nit1 and NitM mutants of each V, dahliae race-2 isolates were paired with VCGJ testers. All isolates of V. dahliae race-2 showed a strong reaction with VCGJ2, i.e., tomato pathotype. All isolates of V. dahliae race-2 except for isolate To22 reacted weakly to VCGJ1 and J3. Japanese isolates of V. dahliae race-2 were assigned as VCGJ2 and were hence vegetatively closely related with those of V. dahliae race-1. The origin of Japanese isolates of V. dahliae race-2 was discussed.

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How do Japanese isolates of Verticillium dahliae correspond with standardized VCG testers?

et al. 1999

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We examined the vegetative compatibility of 56 Japanese isolates provisionally assigned to four subgroups of V, dahliae to estimate the genetic relatedness with testers of the standardized VCGs. Subgroup J1 was assigned to VCG 2A/B as a new category of assignment. Subgroup J2, except isolate Vdt 110, was assigned to VCG 2A, and subgroup J3, except isolate Vdf 1, was assigned to VCG 2B. Isolates Vdf 1 and Vdt 110 were assigned to VCG 2A/B. Subgroup J4 was assigned to two subgroups, VCG 4B for Vde 1 and VCG 4A/B for FY 3 and HR 1. Four isolates were compatible with both VCG 2 and 4. Isolate U56 was compatible with VCG 2A/B and 4A. Isolates of VCG 2A, Vdt 9 and FF 1, were compatible with either VCG 4A or 4A/B. One isolate of VCG 2B, Vdp-4, was compatible with VCG 4A. Three isolates of subgroup J2 showed weak reactions with the testers of VCG 4. These isolates may be "bridging strains". Japanese isolates were composed of two VCGs, 2 and 4, "bridging strains" compatible with these VCGs, and some self-incompatible isolates. Testers of VCG 1 and VCG 3 did not show any reactions with the Japanese isolates.Key Words bridging strain; pathotype; standardized tester; VCG; Verticillium dahliae.Verticillium dahliae Kleb. is one of the important soilborne fungal plant pathogens. It infects a wide range of crops. As the pathogenicity depends upon the isolate (Bewley, 1922;Kendrick and Middleton, 1959), inoculation tests are required to determine the pathogenicity. In Japan, a differential host method has been proposed (lijima, 1983) and improved to determine certain host ranges (Hagiwara, 1990;Horiuchi et al., 1990;Suwa et al., 1987). However, this differential host method has not proven conclusive. Over time, as new differential hosts were used for the pathogenicity test, new categorizations resulted (lijima, 1983;Takeda et al., 1988). Recently, V, dahliae race-2 of tomato wilt (Kobayashi et al., 1989) and tomato-pepper pathotype of V. dahliae (lijima, 1983;Oshima et al., 1993) appeared as new diseases in Japan. Now, determination of pathotypes of V. dahliae has become quite complicated using the differential host method.On the other hand, genetic relatedness in fungi can be estimated by their vegetative compatibility (Glass and Kuldau, 1992;Leslie, 1993 Puhalla and Hummel (1983). By this method, the number of distinct VCGs was reduced from 16 to 4. Several authors have followed this system (Bao et al., 1998;Daayf et al., 1995;Dobinson et al., 1998;Elena, 1997;Korolev, et al., 1997aKorolev, et al., , 1997bRataj-Guranowska and Hiemsta, 1997;Strausbaugh et al., 1992;Strausbaugh, 1993). It should be emphasized that the method used in VCG analysis can affect the results significantly (Rowe, 1995).Evaluation of weak reactions led to subdivisions of VCGs 2 and 4. Joaquim and Rowe (1991) discussed differential reactions among isolates of VCG 2 but avoided the subdivision of this VCG in order to keep their VCG system for V. dahliae as simple as possible. In the case of VCG 4, three subgroups (4A, 4B, and 4A/B) were proposed to accomod...

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Vegetative compatibility groups of Japanese isolates of Verticillium dahliae

Cited by 17 publications

References 19 publications

Vegetative compatibility of an isolate of Verticillium dahliae pathogenic to both tomato and pepper

Vegetative compatibility of an isolate of Verticillium dahliae pathogenic to both tomato and pepper

Assessment of vegetative compatibility of race-2 tomato wilt isolates of Verticillium dahliae in Japan

How do Japanese isolates of Verticillium dahliae correspond with standardized VCG testers?

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