Vegetative Propagation of Eureka Seedless Lemon (Citrus limon L.Cv Eureka seedless) using Different Types of Stem Cutting and Concentrations of Indole-3-Butyric Acid in Winter

Abstract

Citrus can be propagated successfully through stem cutting. However, success of the propagation is depends on various internal as well as external factors. The 2*7 factorial Randomized Complete Block Design (RCBD) experiment was conducted at the Institute of Agriculture and Animal Sciences, Paklihawa Campus, Rupandehi, Nepal from 22 November 2019 to 5 March 2020 to assess the interaction effect of types of stem cuttings and indole 3 butyric acid (IBA) concentrations on the vegetative propagation of Eureka seedless lemon in winter. Stem cutting types had two levels: hardwood and semi-hardwood cutting and IBA concentrations had seven different level i.e control, 500 ppm, 1000 ppm, 2000 ppm, 3000 ppm, 4000 ppm, and 5000 ppm respectively forming overall 14 treatments and three replications. Statistically, significant interaction effects were seen in root parameters. The highest total number of the root(18.00) were found in hardwood cuttings treated with 4000 ppm and lowest total number of roots(1.66) was found in semi-hardwood cuttings treated with 0 ppm of IBA. A statistically significant interaction effect was not found in most of the shoot parameters. However, maximum sprouting (93.33%), the average number of shoots (4.67), average length of the shoot (7.86), and minimum days for first sprouting (30.67 days) were found in hard wood cuttings treated with 4000 ppm IBA. Considering different root and shoot parameters, hardwood cuttings treated with 4000 ppm performed best for root and shoot development. Thus, hardwood cuttings treated with 4000 ppm IBA can be appropriate method for easy and early propagation of eureka seedless lemon.

Plant Growth Regulators and Rooting Media: A Viable Approach for Growth and Performance of Citrus

“…According to the result, the maximum sprouting percentage was observed at 4000 ppm IBA concentration. Along with that, the days for sprouting were about 33.16 [23].…”

“…The study of Gnawali et al, (2022) demonstrates the vegetative growth of Citrus limon in the treatment of IBA in various doses. An experiment was designed to observe sprouting percentage, day of sprouting, length of leaves and shoots, etc.…”

Evaluation of Potato Varieties Rooted Shoot Tip Cuttings as Influenced by IBA Growth Regulator for Transplant Survival and Tuber Production in Northwestern Ethiopia

“…Plant growth regulator is used for transplant survival, establishment, and tuber production of potato by shoot-tip cutting. However, effective exogenous auxin treatments for successful transplant survival, establishment, and tuber production depend on many factors including type of auxin applied, type of variety treated, and number of nodes on the cutting (cutting length), the age of the mother plant when the cuttings are taken and concentration of exogenous auxin applied (Gnawali et al, 2022). Yasser et al ( 2018) reported that the application of 6000 ppm of IBA gave best growth and yield of potato.…”