VEGF and bFGF increase survival of xenografted human ovarian tissue in an experimental rabbit model

Effect of recombinant human vascular endothelial growth factor on testis tissue xenotransplants from prepubertal boys: a three-case study

“…Administration of VEGF, either by culture of the tissue before transplantation or by hydrogel delivery, promoted angiogenesis, follicular survival and enhanced the function of ovarian tissue xenotransplants, including human (Shikanov et al, 2011;Labied et al, 2013;Wang et al, 2013;Henry et al, 2015;Langbeen et al, 2016;Tavana et al, 2016). A previous study on autotransplantation of mouse ITT revealed a positive short-term effect of VEGF on transplant neovascularization 5 and 21 days post-transplantation (Poels et al, 2016), whereas the enhancement of germ cell survival has been demonstrated in several species, including mouse, pig and bovine (Schmidt et al, 2006;Caires et al, 2009Caires et al, , 2012Dores and Dobrinsky, 2014;Poels et al, 2016, Tian et al, 2016.…”

Transplantation of testicular tissue in alginate hydrogel loaded with VEGF nanoparticles improves spermatogonial recovery

“…Indeed, it was established that in the course of revascularization of ovarian tissue grafts, hypoxia occurred during the first 5 days, followed by progressive reoxygenation after this period [11], and that ischemic stress led to tissue apoptosis or necrosis [12]. Hence, different approaches to minimize the side effects of ischemia, such as administration of antioxidants and growth factors including vascular endothelial growth factor (VEGF), either locally or systemically, have been studied in ovarian transplants [13,14]. Data on testicular tissue transplantation outcomes or means of improvement are very limited,…”

In vitro evaluation of the anti-apoptotic drug Z-VAD-FMK on human ovarian granulosa cell lines for further use in ovarian tissue transplantation

“…Moreover, in a previous study, granulosa cells, more so than oocytes, were identified as the main cells affected by the freeze/thaw processing of ovarian tissue [13]. Granulosa cells seem to be able to respond to hypoxia by increasing their synthesis of vascular endothelial growth factor (VEGF) [14], which is an important angiogenic factor that could reduce the hypoxic period post-transplantation, as previously shown in ovarian grafting studies [11,[15][16][17][18][19][20]. Because of the restricted availability and relatively short life span of primary cultures of granulosa cells, human granulosa cell lines were developed to facilitate research [21].…”