VEGF and CD 34: A correlation between tumor angiogenesis and microvessel density-an immunohistochemical study

Kukreja, Ipsita; Kapoor, Prakhar; Deshmukh, Revati; Kulkarni, Venkatesh

doi:10.4103/0973-029x.125200

Cited by 34 publications

(22 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Our results showed that CD24 expression was closely related to MVD, especially in liver metastasis tissues of human CRC patients. These datas suggested that CD24 possiblely promoted the CRC metastasis by regulating the expression of VEGF, which was responsible for tumor MVD [34]. We further confirmed that alerted CD24 expression could affect CRC cells migration, invasion and tubule formatting.…”

Section: Discussionsupporting

confidence: 72%

CD24 promoted cancer cell angiogenesis via Hsp90-mediated STAT3/VEGF signaling pathway in colorectal cancer

et al. 2016

View full text Add to dashboard Cite

CD24 is involved in tumor progression of various cancers, but the effects of CD24 on tumor angiogenesis in colorectal cancer are still unknown. We aimed to investigate the underlying mechanism and role of CD24 on colorectal cancer (CRC) angiogenesis. Our data showed that the microvessal density (MVD) was related to the expression of CD24 in primary and metastasis CRC. Silencing of CD24 could dramatically decrease human umbilical vein endothelial cell (HUVEC) migration, invasion and tubule formation, but trivially affected cell proliferation. We also mechanically showed that silencing CD24 could downregulate the expression of VEGF via inhibiting the phosphorylation and translocation of STAT3. Moreover, Hsp90 was identified as the down-interaction protein of CD24 with co-immunoprecipitation assay and systematic mass spectrometry. Immunofluorescence results showed Hsp90 partly co-localized with CD24 in CRC cell membrane and there was a positive correlation between CD24 and Hsp90 expression in CRC tissues. We gradually evidenced that Hsp90 modulated the stability and degradation of CD24 in a proteasome-depended manner, and transferred the signal transmission from CD24 to STAT3. 17-AAG, a specific Hsp90, could abrogate the CD24 induce- HUVEC migration, invasion and tubule formation in vitro and in vivo. Collectively, our results suggested that CD24 induced CRC angiogenesis in Hsp90-dependent manner and activated STAT3-mediated transcription of VEGF. We provided a new insight into the regulation mechanism of tumor angiogenesis by exploring the role of CD24 in angiogenesis.

show abstract

Section: Discussionsupporting

confidence: 72%

CD24 promoted cancer cell angiogenesis via Hsp90-mediated STAT3/VEGF signaling pathway in colorectal cancer

et al. 2016

View full text Add to dashboard Cite

show abstract

“…These results suggest that RAGE may act as a potential regulatory factor of microvessel formation in endometrial cancer, although a similar phenomenon has previously been observed in renal cell carcinoma [ 6 ] and colorectal cancer [ 7 ]. Folkman (1971) first proposed the concept of angiogenesis-dependent tumour growth [ 17 ], as once the original blood supply is exhausted, the tumour cannot grow without further blood supply [ 18 , 19 ]. The evidence accumulated to date indicates a direct link between microvessel status and cancer development, and a growing body of data suggests that microvessel density is potentially involved in tumour recurrence, metastasis, and survival [ 9 , 10 , 20 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of receptor for advanced glycation endproducts on microvessel formation in endometrial cancer

Zheng

Zhou

et al. 2016

BMC Cancer

View full text Add to dashboard Cite

BackgroundThe receptor for advanced glycation endproducts (RAGE) and microvascular status both play a critical role in cancer progression. However, the crosstalk between RAGE and microvascular formation in endometrial cancer remains largely unknown.MethodsRAGE expression and microvessel density were examined in 20 cases of normal endometrial tissue, 37 cases of well-differentiated endometrial cancer tissue, and 35 cases of poorly-differentiated endometrial cancer tissue. Regression analysis was used to examine the relationship between RAGE and microvessel density. The knockdown of RAGE was achieved using a small interfering RNA in HEC-1A endometrial cancer cells. A xenografted tumour model was used to evaluate RAGE-mediated microvascular formation and proliferation of endometrial cancer cells.ResultsIt was shown that (i) RAGE expression gradually increased in normal endometrium, well-differentiated endometrial cancer, and poorly-differentiated endometrial cancer, respectively; (ii) a positive correlation existed between RAGE and microvessel density in human endometrial cancer samples; (iii) RAGE knockdown was effective in decreasing microvessel formation in xenografted tumour models; and (iv) RAGE knockdown can significantly inhibit the proliferation of endometrial cancer cells in vivo.ConclusionsThese results indicate that RAGE may be a potential trigger in microvascular formation and proliferation in the development of endometrial cancer.Electronic supplementary materialThe online version of this article (doi:10.1186/s12885-016-2126-3) contains supplementary material, which is available to authorized users.

show abstract

“…Few studies have reported that MVD acts as an independent prognostic factor and is also not associated with tumor grade. [ 53 ]…”

Section: Discussionmentioning

confidence: 99%

Evaluation of histomorphological and immunohistochemical parameters as biomarkers of cervical lymph node metastasis in squamous cell carcinoma of oral cavity: A retrospective study

Suresh

Hemalatha

Kumar

et al. 2015

J Oral Maxillofac Pathol

View full text Add to dashboard Cite

Introduction:Oral squamous cell carcinoma (OSCC) is seen worldwide but is more common in India. Lymph node (LN) metastasis has been shown to be the strongest prognostic factor in OSCC. Many histopathological and immunohistochemical markers have been studied to predict the LN metastasis.Aims:To identify clinicopathological factors and immunohistochemical (IHC) biomarkers which predict cervical metastasis in OSCC patients.Study and Design:A total of 105 cases of OSCC were taken up for our study. Histopathological parameters such as tumor thickness, depth, degree of differentiation, pattern of invasion (POI), lymphovascular and neural invasion were assessed. IHC was done on all cases using antibodies against Ki-67, cyclin D, E-cadherin, p53, CD31 and each antibody was assessed according to the standard protocol.Statistical Analysis:To calculate the relation between clinical, histopathological parameter, IHC marker and the occurrence of LN metastasis, chi-square test was used. Variables were tested using multivariate logistic regression method to assess the predictive significance.Results:Out of 105 cases studied, 29 cases showed LN metastasis. Maximum numbers of cases affected were females with involvement of buccal mucosa. We found significant association of cervical LN metastasis with high grade of differentiation, lack of E-cadherin expression, high Ki-67 and cyclin D1 expression. In our study; tumor depth, thickness, extent of peritumoral lympho-plasmacytic infiltration, presence of eosinophils, tumor nest type, p53 and microvessel density (MVD) showed no significant correlation.Conclusion:Significant association of cervical LN metastasis with high grade of differentiation, lack of E-cadherin expression, high Ki-67 and cyclin D1 expression was seen.

show abstract

VEGF and CD 34: A correlation between tumor angiogenesis and microvessel density-an immunohistochemical study

Cited by 34 publications

References 16 publications

CD24 promoted cancer cell angiogenesis via Hsp90-mediated STAT3/VEGF signaling pathway in colorectal cancer

CD24 promoted cancer cell angiogenesis via Hsp90-mediated STAT3/VEGF signaling pathway in colorectal cancer

Effects of receptor for advanced glycation endproducts on microvessel formation in endometrial cancer

Evaluation of histomorphological and immunohistochemical parameters as biomarkers of cervical lymph node metastasis in squamous cell carcinoma of oral cavity: A retrospective study

Contact Info

Product

Resources

About