VEGF and VEGFB Play Balancing Roles in Adipose Differentiation, Gene Expression, and Function

Jin, Honghong; Li, Dan; Wang, Xutong; Jia, Jia; Yang, Chen; Yao, Yapeng; Zhao, Chunlan; Lü, Xiaodan; Zhang, Shujie; Togo, Jacques; Ji, Yan; Zhang, Luqing; Feng, Xuechao; Zheng, Yaowu

doi:10.1210/en.2017-03246

Cited by 34 publications

(23 citation statements)

References 39 publications

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“…7 ) but little identity at the nucleotide sequence level (data not shown). VEGFa and VEGFb play a balance role in adipose differentiation, gene expression and function in energy metabolism 58 . Plant compounds that can down-regulate VEGF expression may have nutritional and therapeutic value.…”

Section: Discussionmentioning

confidence: 99%

Cottonseed-derived gossypol and ethanol extracts differentially regulate cell viability and VEGF gene expression in mouse macrophages

Cao

Sethumadhavan

et al. 2021

Sci Rep

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Vascular endothelial growth factor (VEGF) plays an important role in chronic inflammation associated with several diseases. Many plant extracts have nutritional and healthy benefits by down-regulating VEGF expression, but there was no report on VEGF regulation by cottonseed extracts in any biological system. The objective was to investigate cell viability and VEGF expression regulated by gossypol and ethanol extracts using lipopolysaccharides (LPS) as a control. MTT, qPCR and immunoblotting techniques were used to monitor cell viability, VEGF mRNA and protein levels in mouse RAW264.7 macrophages. Gossypol dramatically reduced macrophage viability but cottonseed extracts and LPS exhibited minor effect on cell viability. VEGFb mRNA levels were approximately 40 fold of VEGFa in the macrophages. Gossypol increased VEGFa and VEGFb mRNA levels up to 27 and 4 fold, respectively, and increased VEGF protein. LPS increased VEGFa mRNA by sixfold but decreased VEGFb mRNA. LPS increased VEGF protein in 2–4 h but decreased in 8–24 h. Glanded seed extracts showed some stimulating effects on VEGF mRNA levels. Glandless seed coat extract showed increased VEGFb mRNA levels but its kernel extract reduced VEGF mRNA levels. This study demonstrated that gossypol and ethanol extracts differentially regulated cell viability and VEGF expression in mouse macrophages.

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Section: Discussionmentioning

confidence: 99%

Cottonseed-derived gossypol and ethanol extracts differentially regulate cell viability and VEGF gene expression in mouse macrophages

Cao

Sethumadhavan

et al. 2021

Sci Rep

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“…PlGF, on the other hand, is thought to be selectively involved in pathological angiogenesis, for instance, in tumors and in ischemic and inflammatory processes [4,5]. VEGF-B is more involved in the growth, differentiation, and survival of certain types of cells [6,7], while VEGF-C and VEGF-D are primarily implicated in lymphangiogenesis [8,9].…”

Section: Introductionmentioning

confidence: 99%

Role of Vascular Endothelial Growth Factor (VEGF) in Human Embryo Implantation: Clinical Implications

Guo

et al. 2021

Biomolecules

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Vascular endothelial growth factor (VEGF) is a well-known angiogenic factor that plays a critical role in various physiological and pathological processes. VEGF also contributes to the process of embryo implantation by enhancing embryo development, improving endometrial receptivity, and facilitating the interactions between the developing embryo and the endometrium. There is a correlation between the alteration of VEGF expression and reproductive failure, including recurrent implantation failure (RIF) and recurrent miscarriage (RM). In order to clarify the role of VEGF in embryo implantation, we reviewed recent literature concerning the expression and function of VEGF in the reproductive system around the time of embryo implantation and we provide a summary of the findings reported so far. We also explored the effects and the possible underlying mechanisms of action of VEGF in embryo implantation.

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“…The synergistic effect on angiogenesis of VEGF from both PRP and fat may be a factor in combined wound‐healing benefits, although there is little literature to support this hypothesis. VEGF also has a complex role in adipocyte survival and differentiation but the exact function is not understood …”

Section: Growth Factors and Their Role In Prp And Fat Synergymentioning

confidence: 99%

“…VEGF also has a complex role in adipocyte survival and differentiation but the exact function is not understood. 127 Transforming growth factor beta 1 (TGF) is involved in all stages of wound healing including angiogenesis, inflammation, cell differentiation, and extracellular matrix deposition. 128,129 Experimental models have shown that there is down regulation of TGF-signalling pathways in chronic non-healing wounds.…”

Section: Growth Factors and Their Role In Prp And Fat Synergymentioning

confidence: 99%

The use of fat grafting and platelet‐rich plasma for wound healing: A review of the current evidence

Smith

Jell

Mosahebi

2018

International Wound Journal

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Fat grafting is becoming a common procedure in regenerative medicine because of its high content of growth factors and adipose derived stem cells (ADSCs) and the ease of harvest, safety, and low cost. The high concentration of ADSCs found in fat has the potential to differentiate into a wide range of wound‐healing cells including fibroblasts and keratinocytes as well as demonstrating proangiogenic qualities. This suggests that fat could play an important role in wound healing. However retention rates of fat grafts are highly variable due in part to inconsistent vascularisation of the transplanted fat. Furthermore, conditions such as diabetes, which have a high prevalence of chronic wounds, reduce the potency and regenerative potential of ADSCs. Platelet‐rich plasma (PRP) is an autologous blood product rich in growth factors, cell adhesion molecules, and cytokines. It has been hypothesised that PRP may have a positive effect on the survival and retention of fat grafts because of improved proliferation and differentiations of ADSCs, reduced inflammation, and improved vascularisation. There is also increasing interest in a possible synergistic effect that PRP may have on the healing potential of fat, although the evidence for this is very limited. In this review, we evaluate the evidence in both in vitro and animal studies on the mechanistic relationship between fat and PRP and how this translates to a benefit in wound healing. We also discuss future directions for both research and clinical practice on how to enhance the regenerative potential of the combination of PRP and fat.

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VEGF and VEGFB Play Balancing Roles in Adipose Differentiation, Gene Expression, and Function

Cited by 34 publications

References 39 publications

Cottonseed-derived gossypol and ethanol extracts differentially regulate cell viability and VEGF gene expression in mouse macrophages

Cottonseed-derived gossypol and ethanol extracts differentially regulate cell viability and VEGF gene expression in mouse macrophages

Role of Vascular Endothelial Growth Factor (VEGF) in Human Embryo Implantation: Clinical Implications

The use of fat grafting and platelet‐rich plasma for wound healing: A review of the current evidence

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