VEGF-C and VEGF-D overexpression is more common in left-sided and well-differentiated colon adenocarcinoma

Szajewski, Mariusz; Kruszewski, Wiesław Janusz; Lakomy, Joanna; Ciesielski, Maciej; Kawecki, K; Jankun, Jerzy; Buczek, Tomasz; Szefel, Jarosław

doi:10.3892/or.2013.2821

Cited by 10 publications

(11 citation statements)

References 35 publications

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“…At the same time pT3-4 poorly differentiated and mucinous tumours were found significantly more frequently in the right side of the colon. Similar results were obtained in another study evaluating simultaneous expression of VEGF-C and VEGF-D in colon pT1-4 cancer cells [ 16 ]. Differences in clinical parameters, histological differentiation, and prognosis in cancers located in the left and right sides of the colon are discussed in many papers.…”

Section: Discussionsupporting

confidence: 89%

“…1 ), which show better prognosis for pT3-4 tumours with present VEGF-C expression (left-sided, moderately and well differentiated tumours). In a previously published paper, we analysed simultaneous expression of lymphangiogenic factors (VEGF-C and VEGF-D) in colon pT1-4 cancer cells [ 16 ]. Both VEGF-C and VEGF-D are involved in the formation of lymphatic microvessels, and through the capability of VEGFR-2 receptor binding they also mediate neovascularisation [ 5 ].…”

Section: Discussionmentioning

confidence: 99%

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VEGF-C expression is not a prognostic factor in locally advanced colon adenocarcinoma

Szajewski

Kruszewski

Lakomy

et al. 2015

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Aim of the studyEvaluation of the relationships between increased expression of VEGF-C (vascular endothelial growth factor-C) and vessel density in the tumour-surrounding stroma, patient survival, and other conventional prognostic factors in patients with pT3-4 colon cancer.Material and methodsExpression of VEGF-C and vessel density were immunohistochemically assessed in 104 specimens of primary, locally advanced (pT3-4) colon adenocarcinoma after surgical resection.ResultsA significant relationship was found between the expression of VEGF-C and increased vessel density in the tumour-surrounding stroma (p = 0.03). A relationship between VEGF-C expression and location of the tumour in the left side of the colon was also found (p = 0.003). Expression of VEGF-C was likely to occur in well-differentiated tumours. No relationship between patient overall survival and the expression level of VEGF-C in locally advanced colon cancer was observed.ConclusionsThe study results indicate that expression of VEGF-C in cells of locally advanced pT3-4 adenocarcinoma of the colon does not affect the survival time of the patients. Increased expression of VEGF-C is accompanied by a significant increase in vessel density in the pT3-4 tumour stroma. Increased expression of VEGF-C in cancer cells is related to the tumour location in the left side of the colon and better tumour differentiation.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

VEGF-C expression is not a prognostic factor in locally advanced colon adenocarcinoma

Szajewski

Kruszewski

Lakomy

et al. 2015

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“…Vascular endothelial growth factor (VEGF) is endothelial cell specific heparin-binding growth factor, which is critical for the proliferation and metastatic potential of colon carcinoma cells [ 13 , 14 ]. We further detected the VEGF protein expression and found that its expression was decreased in p-miR-21-ASO transfected group compared with p-Cont transfected group (Fig.…”

Section: Resultsmentioning

confidence: 99%

Antisense oligonucleotides against microRNA-21 reduced the proliferation and migration of human colon carcinoma cells

Tao

Wen

et al. 2015

Cancer Cell Int

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BackgroundColon carcinoma is one of the commonly tumors that threaten human beings as its highly morbidity and mortality. Recent evidences suggested that microRNA-21 (miR-21) played an important role in the development of colon carcinoma and might be a potential biological marker for the diagnosis and prognosis of colon carcinoma. However, the potential effect of miR-21 based therapeutic studies in colon carcinoma remains to be fully elucidated.MethodsIn present study, we constructed an eukaryotic expression vector encoding antisense oligonucleotides against miR-21 (termed as p-miR-21-ASO) and the expression of miRNA-21 in human colon cancer was detected by Real-time PCR. To assess its possible effect on the proliferation and migration capacity of human colon carcinoma cells in vitro, CCK-8 assay, colony formation assay and cell invasion, as well as migration assay, were performed respectively. Moreover, PTEN, one of target molecules of miRNA-21, was analyzed by Western blot and Fluorescence activated cell sorter assay. Finally, the transduction of AKT and ERK pathways in human colon carcinoma cells was determined by Western blot.ResultsWe found that transiently transfection of p-miR-21-ASO could efficiently decrease the relative expression of miR-21 in human colon carcinoma HCT116 cells, accompanied by impaired proliferation and clone formation. Furthermore, we found that down-regulation of miR-21 also could significantly abrogate the invasion and migration capacity in vitro, as well as the expression of vascular endothelial growth factor which is critical for the metastatic capacity of colon carcinoma cells. Mechanistic evidence showed that down-regulation of miR-21 increased the expression of its target molecule PTEN in HCT116 cells. Finally, we revealed that the expression level of both phosphor-ERK1/2 and phosphor-AKT also were altered.ConclusionsTherefore, our data suggested miR-21 ASO against miR-21 might be a useful strategy to alter the expression of miR-21 in colon carcinoma cells, which was helpful for the development of miR-21-based therapeutic strategies against clinical colon carcinoma.Electronic supplementary materialThe online version of this article (doi:10.1186/s12935-015-0228-7) contains supplementary material, which is available to authorized users.

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“…Formation of tumor vessel network, including blood and lymph vessels, is an important stage in carcinogenesis process. The discovery of vascular growth factors has resulted into a better understanding of tumor biology, so opening new possibilities in treatment of cancer to target angiogenesis within tumor-associated stroma, including therapy for colon cancer patients is of great importance (Szajewski et al, 2014).…”

Section: Clinical Significance Of Serum Vascular Endothelial Growth Fmentioning

confidence: 99%

Clinical Significance of Serum Vascular Endothelial Growth Factor and Complement 3a Levels in Patients with Colorectal Cancer in Southern Iran

Mehrabani

Shamsdin²,

Dehghan³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Background: Colon cancer (CRC) is perhaps the second most common cause of cancer mortality. This study determined the clinical significance of serum vascular endothelial growth factor (VEGF) and serum complement 3a (C3a) levels in patients with CRC in Fars province, southern Iran. Materials and Methods: Between June 2010 and June 2012, 110 patients with CRC of both genders and different age groups were divided into 3 groups. Group A included patients who had just undergone surgery; Group B had undergone chemotherapy after surgery; and Group C had undergone chemotherapy and radiotherapy after surgery. Twenty one healthy subjects with normal colonoscopy were considered as a control group. ELISA was undertaken to determine VEGF and C3a levels before and after treatment measures. Results: The mean age of patients was 53.9±14.1 years. Considering VEGF level, a significant decrease was visible after treatment measures in groups A and B, but not Group C. For VEGF level, the difference was not statistically significant between two genders and various age groups before and after treatment. No significant difference was found for VEGF level between patients and normal group before any treatment. Regarding C3a levels in 101 subjects, they significantly decreased after treatment measures. Before and after treatment, the difference was statistically significant between two genders, but was not statistically significant among various age groups. Conclusions: As VEGF and C3a levels were significantly lower in patients after treatment, these may be beneficial markers in assessment of CRC therapy especially in early stages.

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VEGF-C and VEGF-D overexpression is more common in left-sided and well-differentiated colon adenocarcinoma

Cited by 10 publications

References 35 publications

VEGF-C expression is not a prognostic factor in locally advanced colon adenocarcinoma

VEGF-C expression is not a prognostic factor in locally advanced colon adenocarcinoma

Antisense oligonucleotides against microRNA-21 reduced the proliferation and migration of human colon carcinoma cells

Clinical Significance of Serum Vascular Endothelial Growth Factor and Complement 3a Levels in Patients with Colorectal Cancer in Southern Iran

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