VEGF mRNA Assessment in Human Pterygium: A New ‘Scope' for a Future Hope

Sava, Mihai G. Poenaru; Raica, Marius; Cimpean, Anca Maria G.

doi:10.1159/000363142

Cited by 7 publications

(5 citation statements)

References 30 publications

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“…Recent research found that miR-21/vascular endothelial growth factor (VEGF) plays an important role in the development of ocular angiogenesis [37]. Previous studies showed that anti-VEGF therapy was effective in some patients with pterygium [38] taking into account that miR-21 is the relative upstream factor and anti-miR-21 may be a possible treatment strategy for pterygium. Of course, a delivery system is required to ensure stability in vivo and to efficiently and safely introduce nucleic acid–based drugs into cells [39].…”

Section: Discussionmentioning

confidence: 99%

miR-21 Promotes Human Nucleus Pulposus Cell Proliferation through PTEN/AKT Signaling

Liu

Xiang-wang

et al. 2014

IJMS

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The precise role of nucleus pulposus cell proliferation in the pathogenesis of intervertebral disc degeneration remains to be elucidated. Recent findings have revealed that microRNAs, a class of small noncoding RNAs, may regulate cell proliferation in many pathological conditions. Here, we showed that miR-21 was significantly upregulated in degenerative nucleus pulposus tissues when compared with nucleus pulposus tissues that were isolated from patients with idiopathic scoliosis and that miR-10b levels were associated with disc degeneration grade. Moreover, bioinformatics target prediction identified PTEN as a putative target of miR-21. miR-21 inhibited PTEN expression by directly targeting the 3′UTR, and this inhibition was abolished through miR-21 binding site mutations. miR-21 overexpression stimulated cell proliferation and AKT signaling pathway activation, which led to cyclin D1 translation. Additionally, the increase in proliferation and cyclin D1 expression induced by miR-21 overexpression was almost completely blocked by Ly294002, an AKT inhibitor. Taken together, aberrant miR-21 upregulation in intervertebral disc degeneration could target PTEN, which would contribute to abnormal nucleus pulposus cell proliferation through derepressing the Akt pathway. Our study also underscores the potential of miR-21 and the PTEN/Akt pathway as novel therapeutic targets in intervertebral disc degeneration.

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Section: Discussionmentioning

confidence: 99%

miR-21 Promotes Human Nucleus Pulposus Cell Proliferation through PTEN/AKT Signaling

Liu

Xiang-wang

et al. 2014

IJMS

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“…Although the exact pathogenesis of pterygium is still unclear, chronic inflammation, angiogenesis, and uncontrolled proliferation seem to be the key mechanisms in progression (1,5,20). Several inflammatory and angiogenic factors, such as VEGF, MMP, and TGF, were suggested to be related to its pathogenesis (21)(22)(23). In the present study, we investigated the cell expressions of COX-2 and CXCR-4 in pterygium tissue.…”

Section: Discussionmentioning

confidence: 94%

Chemokine CXCR-4 and cyclooxygenase-2 in the pathogenesis of pterygium

Baser¹,

Sivrikoz²,

Karahan³

et al. 2017

Turk J Med Sci

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Background/aim: This study aimed to investigate the expression of chemokine receptor 4 (CXCR-4) and cyclooxygenase-2 (COX-2) in the epithelium and stroma of pterygium tissue in comparison with healthy conjunctiva. Materials and methods:The expression of CXCR4 and COX-2 was investigated by immunohistochemistry in the epithelium and stroma of the pterygium tissue of 29 eyes and compared with healthy conjunctival tissues. The correlation between CXCR4 and COX-2 expression as well as the correlation of these markers with the area of pterygium were evaluated statistically.Results: COX-2 staining scores were 1.75 ± 0.63 in the epithelium and 1.20 ± 0.62 in the stroma of the pterygium tissue. Mean CXCR-4 staining in the epithelium was 0.069 ± 0.37, whereas it was 5.0 ± 2.84 cells in the stroma. There was almost no staining of COX-2 and CXCR4 in the control samples. There was a strong positive correlation between the expression of CXCR-4 and COX-2 in the stroma of the pterygium. Conclusion:CXCR-4 and COX-2 may play important roles in the pathogenesis of pterygium.

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“…The detection sensitivity of the RNAscope method has been proven by using positive (POLR2A) and negative controls (probes against the bacterial dap B gene), evaluated using the same protocol applied in our previous studies [ 32 , 33 , 34 , 35 ].…”

Section: Methodsmentioning

confidence: 99%

“…In situ hybridization techniques were applied on human placenta tissue [31] but not for the detection and characterization of PDPN expression/distribution in the stromal and epithelial components of the human placenta. Among these methods, RNAscope is a commercially accessible in situ hybridization (ISH) technique used to identify RNA in formalin-fixed paraffin-embedded tissue [13,[32][33][34]. This technique enables the visualization of single RNA molecules within individual cells.…”

Section: Introductionmentioning

confidence: 99%

“…This technique enables the visualization of single RNA molecules within individual cells. Its efficacy has been examined and confirmed in several human medical conditions [13,[32][33][34]. Crucially, the main distinction from the usual RNA ISH is that RNAScope identifies targetspecific probes, reducing the occurrence of non-specific off-target signals.…”

Section: Introductionmentioning

confidence: 99%

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Combining RNAscope, Immunohistochemistry (IHC) and Digital Image Analysis to Assess Podoplanin (PDPN) Protein and PDPN_mRNA Expression on Formalin-Fixed Paraffin-Embedded Normal Human Placenta Tissues

Tomescu,

Cosma,

Fenesan

et al. 2024

CIMB

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The expression and function of podoplanin (PDPN) in the normal human placenta has been debated in placental evaluation. This study emphasizes the importance of a multimodal approach of PDPN expression in normal human placentas. A complete examination is performed using immunohistochemistry, RNAscope and automated Digital Image examination (DIA) interpretation. QuPath DIA-based analysis automatically generated the stromal and histological scores of PDPN expression for immunohistochemistry and RNAscope stains. The umbilical cord’s isolated fibroblasts and luminal structures expressed PDPN protein and PDPN_mRNA. RNAscope detected PDPN_mRNA upregulation in syncytial placental knots trophoblastic cells, but immunohistochemistry did not certify this at the protein level. The study found a significant correlation between the IHC and RNAscope H-Score (p = 0.033) and Allred Score (p = 0.05). A successful multimodal strategy for PDPN assessment in human placentas confirmed PDPN expression heterogeneity in the full-term human normal placenta and umbilical cord at the protein and mRNA level. In placental syncytial knots trophoblastic cells, PDPN showed mRNA overexpression, suggesting a potential role in placenta maturation.

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VEGF mRNA Assessment in Human Pterygium: A New ‘Scope' for a Future Hope

Cited by 7 publications

References 30 publications

miR-21 Promotes Human Nucleus Pulposus Cell Proliferation through PTEN/AKT Signaling

miR-21 Promotes Human Nucleus Pulposus Cell Proliferation through PTEN/AKT Signaling

Chemokine CXCR-4 and cyclooxygenase-2 in the pathogenesis of pterygium

Combining RNAscope, Immunohistochemistry (IHC) and Digital Image Analysis to Assess Podoplanin (PDPN) Protein and PDPN_mRNA Expression on Formalin-Fixed Paraffin-Embedded Normal Human Placenta Tissues

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