VEGF‐promoted surgical angiogenesis in necrotic bone

Suzuki, Osami; Bishop, Allen T.; Sunagawa, Toru; Katsube, Kosuke; Friedrich, Patricia F.

doi:10.1002/micr.10190

Cited by 39 publications

(28 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies have shown that VEGF gene transfer or direct administration of VEGF can promote surgical angiogenesis in necrotic bone (36)(37)(38); these studies, however, did not provide evidence regarding bone tissue regeneration or reparative osteogenesis. In a rat femoral head model of vessel deprivation-induced osteonecrosis, high VEGF expression accounted for the striking bone resorption-related remodeling of necrotic debris early during repair, with subsequent substitution by newly formed bone (39,40).…”

Section: Discussionmentioning

confidence: 97%

Continuous occurrence of both insufficient neovascularization and elevated vascular permeability in rabbit proximal femur during inadequate repair of steroid‐associated osteonecrotic lesions

Zhang¹,

Sheng²,

He³

et al. 2009

Arthritis & Rheumatism

View full text Add to dashboard Cite

Objective. To examine the features of the intraosseous vasculature, the size of the marrow stem cell pool (MSCP), and expression of vascular endothelial growth factor A (VEGF) during inadequate repair of steroid-associated osteonecrotic lesions in rabbits.Methods. Steroid-associated osteonecrosis was induced in male rabbits. At 0, 1, 2, 4, and 6 weeks postinduction, vascularization and permeability indices were quantified by dynamic magnetic resonance imaging (MRI). In addition, the size of the MSCP in the hematopoietic and mesenchymal compartments was determined, and marrow mononuclear cells expressing specific surface markers for endothelial progenitor cells or periendothelial mural precursor cells were counted. At various time points after the rabbits were killed, the proximal femora were dissected to examine the intraosseous vasculature by angiography, histomorphometry, and ultramorphology. In addition, osteonecrotic lesion repair and marrow VEGF expression were evaluated.Results. Lesion formation without repair was observed at 2 weeks after induction of steroid-associated osteonecrosis. Rabbits displaying destructive repair (DR؉) and those displaying reparative osteogenesis (DR؊) from 4 weeks to 6 weeks postinduction were identified. From week 2 to week 6, the vascularization index was significantly lower in DR؉ rabbits compared with DR؊ rabbits, whereas the permeability index was significantly higher in DR؉ rabbits compared with DR؊ rabbits. The features of the intraosseous vasculature determined by angiography, histomorphometry, and ultramorphology were consistent with those determined by dynamic MRI. The MSCP size and number of marrow mononuclear cells expressing specific surface markers were all significantly lower in DR؉ rabbits than in DR؊ rabbits from week 1 to week 6. The increased VEGF expression at 2 weeks was maintained through week 6 in DR؉ rabbits, whereas VEGF expression decreased in DR؊ rabbits from week 2 to week 6.Conclusion. Continuous occurrence of both insufficient neovascularization and elevated vascular permeability is accompanied by a continuously lowlevel MSCP and uncontrolled VEGF expression during inadequate repair of steroid-associated osteonecrotic lesions.Pulsed steroids are frequently prescribed as lifesaving agents for serious infectious diseases such as severe acute respiratory syndrome and acquired immunodeficiency syndrome, or as disease-modifying agents for chronic autoimmune diseases such as systemic lupus erythematosus and rheumatoid arthritis. However, pulsed administration of steroids often results in osteonecrosis (also known as avascular necrosis). Orthopedic surgeons face the challenge of managing subchondral

show abstract

Section: Discussionmentioning

confidence: 97%

Continuous occurrence of both insufficient neovascularization and elevated vascular permeability in rabbit proximal femur during inadequate repair of steroid‐associated osteonecrotic lesions

Zhang¹,

Sheng²,

He³

et al. 2009

Arthritis & Rheumatism

View full text Add to dashboard Cite

show abstract

“…VEGF acts in a paracrine and autocrine manner on endothelial cells as a heparin-binding homodimeric glycoprotein (1). VEGF gene transduction has also been found to promote bone marrow cell and endothelial cell proliferation, and induce lumen formation in vitro (2,3). Therefore, the VEGF gene is considered a plausible biological candidate for ONFH.…”

Section: Introductionmentioning

confidence: 99%

Association between vascular endothelial growth factor gene polymorphisms and the risk of osteonecrosis of the femoral head: Systematic review

Hong

Chen

et al. 2015

Biomedical Reports

View full text Add to dashboard Cite

Abstract.Emerging evidence has shown that vascular endothelial growth factor (VEGF) gene polymorphisms are the key initiators that regulate the expression of the VEGF protein, which has a vital role in osteonecrosis of the femoral head (ONFH). The aim of the present study was to investigate whether polymorphisms of the VEGF genes are associated with the occurrence of ONFH. A comprehensive search was performed on MEDLINE, Embase, Web of Science and China National Knowledge Infrastructure databases before June 2015. Meta-analyses were carried out for the VEGF gene -634G/C polymorphisms (single-nucleotide polymorphism with 3 eligible studies). The pooled odds ratios with 95% confidence intervals (CIs) were used to evaluate the strength of the association. All the eligible studies, involving 1,564 individuals, were identified. According to the inclusion criteria, 3 case-control studies were finally included in the meta-analysis. The present meta-analysis indicates that the VEGF gene -634G/C polymorphism [CC+GC vs. GG: Response rate (RR)=0.79; 95% CI, 0.67-0.92; GG vs. GC: RR=0.83; 95% CI, GG vs. CC: RR=0.82; 95% CI,] is associated with the occurrence of ONFH, and the association with the male subgroup (RR=0.78; 95% CI, 0.65-0.94; P=0.009) is more evident. In conclusion, the present meta-analysis suggests that the VEGF gene -634G/C polymorphism has a significant association with ONFH occurrence among the investigated patients (P<0.01).

show abstract

“…The alternative is to detect GFP immunochemically using antibody to GFP. Direct VEGF administration can improve blood flow and neovascularization in necrotic bone [38]. In addition to its effects on angiogenesis, VEGF plays an important role in bone growth and repair by interacting with other growth factors [35,36].…”

Section: Limitationsmentioning

confidence: 99%

Emerging Ideas: Treatment of Precollapse Osteonecrosis Using Stem Cells and Growth Factors

Cui

Botchwey

2011

Clinical Orthopaedics &Amp; Related Research

View full text Add to dashboard Cite

show abstract

VEGF‐promoted surgical angiogenesis in necrotic bone

Cited by 39 publications

References 36 publications

Continuous occurrence of both insufficient neovascularization and elevated vascular permeability in rabbit proximal femur during inadequate repair of steroid‐associated osteonecrotic lesions

Continuous occurrence of both insufficient neovascularization and elevated vascular permeability in rabbit proximal femur during inadequate repair of steroid‐associated osteonecrotic lesions

Association between vascular endothelial growth factor gene polymorphisms and the risk of osteonecrosis of the femoral head: Systematic review

Emerging Ideas: Treatment of Precollapse Osteonecrosis Using Stem Cells and Growth Factors

Contact Info

Product

Resources

About