Velvet activated McrA plays a key role in cellular and metabolic development in Aspergillus nidulans

Lee, Mi-Kyung; Son, Ye-Eun; Park, Hee-Soo; Alshannaq, Ahmad; Han, Kap-Hoon; Yu, Jae‐Hyuk

doi:10.1038/s41598-020-72224-y

Cited by 7 publications

(8 citation statements)

References 48 publications

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“…Fifty-five Zn 2 Cys 6 transcription factors, Gal4, Hap1, and Leu3, have been found in S. cerevisiae 16 . Some Zn 2 Cys 6 transcription factors in A. nidulans have been characterized (e.g., AlcA, PrnA, AflR, NirA, SclB, and McrA) [17][18][19][20][21][22] . However, the function of AN5849 has not been investigated.…”

Section: Resultsmentioning

confidence: 99%

“…In the conidiation regulators of A. nidulans, CsgA, McrA, SclB, SfgA, and ZcfA are Zn 2 Cys 6 transcription factors, as well as SrdA. SclB and McrA positively regulate conidiation, whereas CsgA and ZcfA are necessary for proper asexual and sexual development 21,22,40,41 . It has been reported that the heterodimer of the velvet proteins, VosA-VelB, directly binds to the promoter region of the target genes and regulates conidiation and spore viability [42][43][44] .…”

Section: Discussionmentioning

confidence: 99%

“…It has been reported that the heterodimer of the velvet proteins, VosA-VelB, directly binds to the promoter region of the target genes and regulates conidiation and spore viability [42][43][44] . The expression of sclB and mcrA are regulated by the VosA-VelB complex 21,22 . Although SrdA is not a positive regulator, VosA-VelB complex may be involved in regulating srdA expression.…”

Section: Discussionmentioning

confidence: 99%

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srdA mutations suppress the rseA/cpsA deletion mutant conidiation defect in Aspergillus nidulans

Ogawa

Fukuda

Iwama

et al. 2023

Sci Rep

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Conidiation is an important reproductive process in Aspergillus. We previously reported, in A. nidulans, that the deletion of a putative glycosyltransferase gene, rseA/cpsA, causes an increase in the production of extracellular hydrolases and a severe reduction in conidiation. The aim of this study was to obtain novel genetic factors involved in the repression of conidiation in the rseA deletion mutant. We isolated mutants in which the rseA deletion mutant conidiation defect is suppressed and performed a comparative genomic analysis of these mutants. A gene encoding a putative transcription factor was identified as the associated candidate causative gene. The candidate gene was designated as srdA (suppressor gene for the conidiation defect of the rseAdeletion mutant). The conidiation efficiency of the rseAsrdA double-deletion mutant was increased. Introduction of wild-type srdA into the suppressor mutants caused a conidiation defect similar to that of the rseA deletion mutant. Notably, the conidiation efficiencies of the rseAsrdA double-deletion and srdA single-deletion mutants were higher than that of the wild-type strain. These results indicate that srdA is a novel genetic factor that strongly represses conidiation of the rseA deletion mutant, and a putative transcriptional regulator, SrdA is a negative regulator of conidiation in A. nidulans.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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srdA mutations suppress the rseA/cpsA deletion mutant conidiation defect in Aspergillus nidulans

Ogawa

Fukuda

Iwama

et al. 2023

Sci Rep

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“…In A. nidulans , only a few GAL4-like proteins have been studied. For example, McrA, which encodes a GAL4-like Zn 2 Cys 6 domain at the C-terminus, plays a key role in fungal growth, spore viability, and secondary metabolism 36 . ZcfA, a zinc cluster TF containing a GAL4-like Zn 2 Cys 6 binuclear cluster DNA-binding domain, also regulates both asexual and sexual development in A. nidulans 21 .…”

Section: Discussionmentioning

confidence: 99%

The function of a conidia specific transcription factor CsgA in Aspergillus nidulans

Cho

Park

2022

Sci Rep

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Aspergillus spp. mainly reproduce asexually via asexual spores called conidia. In this study, we identified CsgA, a conidia-specific Zn2Cys6 transcription factor containing the GAL4-like zinc-finger domain, and characterized the roles of CsgA in the model organism Aspergillus nidulans. In A. nidulans, the ΔcsgA strain produced abnormal conidiophores and exhibited increased conidial production. The deletion of csgA resulted in impaired production of sexual fruiting bodies (cleistothecia) and lower mutA expression levels. Overexpression of csgA led to decreased conidia production but increased cleistothecia production, suggesting that CsgA is essential for proper asexual and sexual development in A. nidulans. In conidia, the deletion of csgA resulted in increased trehalose content, higher spore viability, and increased tolerance to thermal and oxidative stresses. Transcriptomic analysis revealed that the loss of csgA affects the expression of genes related to conidia germination, DNA repair, and secondary metabolite biosynthesis. Further analysis revealed that the ΔcsgA strain exhibited delayed conidial germination and abnormal germ tube length. Additionally, the production of sterigmatocystin increased in the ΔcsgA conidia compared to that in the controls. Overall, these results suggest that CsgA is crucial for proper fungal development, spore viability, conidial germination, and sterigmatocystin production in A. nidulans.

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“…Sterigmatocystin biosynthesis depends on a gene cluster of which the AflR transcription factor is a key regulator [ 22 - 24 ]. Other important genes involved in sterigmatocystin production include MtfA, VeA, LaeA, and McrA [ 25 - 28 ].…”

Section: Introductionmentioning

confidence: 99%

Functions of PUF Family RNA-Binding Proteins in Aspergillus nidulans

Son

Jang

Park

2021

J. Microbiol. Biotechnol.

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Aspergillus nidulans is a model filamentous fungus widely used in fungal development and metabolism studies [1,2]. A. nidulans propagates through both asexual and sexual spores, but asexual spores are their primary reproductive particle [3,4]. Spores germinate to form germ tubes and a web-like mass of fungal hyphae [5]. These hyphae acquire developmental competence and undergo asexual or sexual development, depending on the environmental conditions [6,7]. For example, light can induce the formation of asexual structures-the conidiophores-but sexual fruiting bodies-the cleistothecia-are formed in dark conditions [8,9]. These reproductive processes are regulated by a myriad of transcription factors and several signaling pathways [10,11]. In asexual development, BrlA, AbaA, and WetA are central transcription factors that control mRNA expression of development-related genes [6,12]. BrlA is a C 2 H 2 zinc-finger transcription factor that controls mRNA levels of abaA and other developmental genes [13]. AbaA induces mRNA expression of three key transcription factors: WetA, VosA, and VelB [14][15][16]. These transcription factors regulate asexual spore maturation, trehalose biosynthesis, and conidial stress tolerance [15,17]. Fungal species produce various primary and secondary metabolites during development [18]. Secondary metabolites that have detrimental effects on humans are called mycotoxins [19,20]. Sterigmatocystin produced by A. nidulans is a precursor aflatoxin and is considered as a group 2B carcinogen [21]. Sterigmatocystin biosynthesis depends on a gene cluster of which the AflR transcription factor is a key regulator [22][23][24]. Other important genes involved in sterigmatocystin production include MtfA, VeA, LaeA,.RNA-binding proteins play essential roles in the posttranscriptional regulation of gene expression in eukaryotes [29][30][31]. In fungi, RNA-binding proteins participate in various fundamental processes, including development, stress responses, filamentation, and pathogenesis [32,33]. The PUF (Pumilio/Fem-3 binding factor) family of canonical RNA-binding proteins is conserved among most eukaryotic systems [34]. The Pumilio in Drosophila melanogaster and Fem-3 binding factor (FBF) in Caenorhabditis elegans are the founding members of the PUF family proteins [35]. They contain several repeated PUF domains, which interact with sequencespecific RNA elements of their target. [35]. In Ascomycota fungi, PUF proteins recognize 8-to 10-nucleotide binding elements, reducing translational efficiency or by increasing its decay [36,37]. Most of the research on the RNA targets and the biological roles of the PUF proteins have been carried out in Saccharomyces cerevisiae [35,38,39]. S. cerevisiae contains six PUF proteins involved in posttranscriptional processes, mating type switching, lifespan, thermotolerance, cell wall integrity, and mitochondrial biogenesis [35,40]. For example, PUF6 targets RNA-binding proteins are involved in RNA metabolism and posttranscriptional regulation of various fundamental biolog...

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Velvet activated McrA plays a key role in cellular and metabolic development in Aspergillus nidulans

Cited by 7 publications

References 48 publications

srdA mutations suppress the rseA/cpsA deletion mutant conidiation defect in Aspergillus nidulans

srdA mutations suppress the rseA/cpsA deletion mutant conidiation defect in Aspergillus nidulans

The function of a conidia specific transcription factor CsgA in Aspergillus nidulans

Functions of PUF Family RNA-Binding Proteins in Aspergillus nidulans

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