Venom from Opisthacanthus elatus scorpion of Colombia, could be more hemolytic and less neurotoxic than thought

Estrada-Gómez, Sebastián; Muñoz, Leidy Johana Vargas; Saldarriaga‐Córdoba, Mónica; Castillo, Juan Carlos Quintana

doi:10.1016/j.actatropica.2015.09.019

Cited by 13 publications

(17 citation statements)

References 46 publications

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“…Top-down data sets showed that the venom of B.occitanus is very complex, counting around 200 molecular weights ranging from 1868 to 16 720 Da. A similar number of components have been revealed by previous studies [32,33,34], others showed fewer components, as well as Leiurus abdullahbayrami (45 masses) and Opisthacanthus elatus (106 masses) [35,36], whereas some others scorpion venoms were more complex, such as the Pandinus cavimanus (390 masses) and…”

Section: Discussionsupporting

confidence: 82%

“…Top‐down data sets showed that the venom of B. occitanus is very complex, counting around 200 MWs ranging from 1868 to 16 720 Da. A similar number of components have been revealed by previous studies [32–34], others showed fewer components, as well as Leiurus abdullahbayrami (45 masses) and Opisthacanthus elatus (106 masses) [35, 36], whereas some other scorpion venoms were more complex, such as the Pandinus cavimanus (390 masses) and Centruroides limpidus (395 masses) [37, 38]. Additionally, the repartition of MWs showed that < 1% were components with molecular masses < 2000 Da, 14% were those from 2000 to 5000 Da, 74% were those between 5000 and 8000 Da, and 10% were those over than 8000 Da, while the repartition of MW from the French B. occitanus scorpion venom showed an abundance of molecules ranging from 2000 to 3000 Da and those less than 2000 Da [39].…”

Section: Discussionsupporting

confidence: 73%

“…Additionally, the repartition of molecular weights showed that less than 1% were components with molecular masses <2000 Da, 14% those from 2000 to 5000 Da, 74% those between 5000 to 8000 Da, and 10% were those over than 8000 Da, while the repartition of molecular weights from the French B.occitanus scorpion venom showed an abundance of molecules ranging from 2000 to 3000 Da and those less than 2000 Da [39]. revealed by previous studies [32,33,34], others showed fewer components, as well as Leiurus abdullahbayrami (45 masses) and Opisthacanthus elatus (106 masses) [35,36] whereas some others scorpion venoms were more complex, such as the Pandinus cavimanus (390 masses) and…”

Section: Discussionmentioning

confidence: 82%

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Mass spectrometry‐based top‐down and bottom‐up approaches for proteomic analysis of the Moroccan Buthus occitanus scorpion venom

et al. 2021

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Buthus occitanus (B.occitanus) is one of the most dangerous scorpions in the world. Despite the involvement of B.occitanus scorpion in severe cases of envenomation in Morocco, no study has focused yet on the proteomic composition of the Moroccan B.occitanus scorpion venom. Mass spectrometry-based proteomic techniques are commonly used in the study of scorpion venoms. The implementation of top-down and bottom-up approaches for proteomic analyses facilitates screening by allowing a global view of the structural aspects of such complex matrices. Here, we provide a partial overview of the venom of B.occitanus, in order to explore the diversity of its toxins and hereafter understand their effects. To this end, a combination of top-down and bottomup approaches was applied using nano-high liquid chromatography coupled to nano-electrospray tandem mass spectrometry (nano-LC-ESI-MS/MS). TheLC-MS results showed that B.occitanus venom contains around 200 molecular masses ranging from 1868 to 16,720 Da, the most representative of which are those between 5000 and 8000 Da. Interestingly, combined top-down and bottom-up LC-MS/MS results allowed the identification of several toxins, which were mainly those acting on ion channels, including those targeting sodium (NaScTxs), potassium (KScTxs), chloride (ClScTxs) and calcium channels (CaScTx), as well as antimicrobial peptides (AMPs), amphipathic peptides, myotropic neuropeptides and hypothetical secreted proteins. This study reveals the molecular diversity of B.occitanus scorpion venom and identifies components that may have useful pharmacological activities.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionsupporting

confidence: 73%

Section: Discussionmentioning

confidence: 82%

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Mass spectrometry‐based top‐down and bottom‐up approaches for proteomic analysis of the Moroccan Buthus occitanus scorpion venom

et al. 2021

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“…No studies have been found describing the venom content of Chactas reticulatus, Opisthacanthus elatus, Centruroides edwardsii or Tityus asthenes . The only available studies report the phospholipase A 2 content and activity in the venom of O. elatus , and the intraspecific biochemical differences detected in the venom of C. edwardsii from two regions in Colombia [ 16 , 17 ]. The genus Tityus is probably one of the must studied scorpions in South America, but from Colombia the only available studies of this genus are the proteomic analysis of Tityus pachyurus reporting specific toxins affecting Na + and K + channels [ 18 , 20 ], and the peptide content description of Tityus macrochirus [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

MS/MS analysis of four scorpion venoms from Colombia: a descriptive approach

Estrada-Gómez

Muñoz

Saldarriaga‐Córdoba

et al. 2021

J. Venom. Anim. Toxins incl. Trop. Dis

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Background: Scorpions are widely known for the neurotoxic effects of their venoms, which contain peptides affecting ionic channels. Although Colombia is recognized for its scorpion diversity, only a few studies are available describing the venom content. Methods: In this descriptive study, we analyzed the MS/MS sequence, electrophoretic and chromatographic profile linked to a bioinformatics analysis of the scorpions Chactas reticulatus (Chactidae), Opisthacanthus elatus (Hormuridae), Centruroides edwardsii (Buthidae) and Tityus asthenes (Buthidae) from Colombia. Results: Each scorpion showed a specific electrophoretic and chromatographic profile. The electrophoretic profiles indicate the presence of high molecular mass compounds in all venoms, with a predominance of low molecular mass compounds in the Buthidae species. Chromatographic profiles showed a similar pattern as the electrophoretic profiles. From the MS/MS analysis of the chromatographic collected fractions, we obtained internal peptide sequences corresponding to proteins reported in scorpions from the respective family of the analyzed samples. Some of these proteins correspond to neurotoxins affecting ionic channels, antimicrobial peptides and metalloproteinase-like fragments. In the venom of Tityus asthenes, the MS n analysis allowed the detection of two toxins affecting sodium channels covering 50% and 84% of the sequence respectively, showing 100% sequence similarity. Two sequences from Tityus asthenes showed sequence similarity with a phospholipase from Opisthacanthus cayaporum indicating the presence of this type of toxin in this species for the first time. One sequence matching a hypothetical secreted protein from Hottentotta judaicus was found in three of the studied venoms. We found that this protein is common in the Buthidae family whereas it has been reported in other families - such as Scorpionidae - and may be part of the evolutionary puzzle of venoms in these arachnids. Conclusion: Buthidae venoms from Colombia can be considered an important source of peptides similar to toxins affecting ionic channels. An interesting predicted antimicrobial peptide was detected in three of the analyzed venoms.

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“…Although focus in this study was on hyaluronidase enzymes, direct inhibition of hyaluronidases was not tested. Other studies have shown, however, that Aristolochic acid has an inhibitory effect on phospholipases A 2 (PLA 2 s), which are found in scorpion venoms [120,132]. To date, no inhibition studies with Aristolochic acid on PLA 2 s from spider venom have been reported, despite the presence of PLA 2 s in arachnid venoms.…”

Section: Toxin Inhibition By Small Moleculesmentioning

confidence: 99%

Biotechnological Trends in Spider and Scorpion Antivenom Development

Laustsen

Solà

Jappe

et al. 2016

Toxins

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Spiders and scorpions are notorious for their fearful dispositions and their ability to inject venom into prey and predators, causing symptoms such as necrosis, paralysis, and excruciating pain. Information on venom composition and the toxins present in these species is growing due to an interest in using bioactive toxins from spiders and scorpions for drug discovery purposes and for solving crystal structures of membrane-embedded receptors. Additionally, the identification and isolation of a myriad of spider and scorpion toxins has allowed research within next generation antivenoms to progress at an increasingly faster pace. In this review, the current knowledge of spider and scorpion venoms is presented, followed by a discussion of all published biotechnological efforts within development of spider and scorpion antitoxins based on small molecules, antibodies and fragments thereof, and next generation immunization strategies. The increasing number of discovery and development efforts within this field may point towards an upcoming transition from serum-based antivenoms towards therapeutic solutions based on modern biotechnology.

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Venom from Opisthacanthus elatus scorpion of Colombia, could be more hemolytic and less neurotoxic than thought

Cited by 13 publications

References 46 publications

Mass spectrometry‐based top‐down and bottom‐up approaches for proteomic analysis of the Moroccan Buthus occitanus scorpion venom

Mass spectrometry‐based top‐down and bottom‐up approaches for proteomic analysis of the Moroccan Buthus occitanus scorpion venom

MS/MS analysis of four scorpion venoms from Colombia: a descriptive approach

Biotechnological Trends in Spider and Scorpion Antivenom Development

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