Venomics and Cellular Toxicity of Thai Pit Vipers (Trimeresurus macrops and T. hageni)

Kumkate, Supeecha; Chanhome, Lawan; Thiangtrongjit, Tipparat; Noiphrom, Jureeporn; Laoungboa, Panithi; Khow, Orawan; Vasaruchapong, Taksa; Sitprija, Siravit; Chaiyabutr, Narongsak; Reamtong, Onrapak

doi:10.3390/toxins12010054

Cited by 9 publications

(9 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This suggests that the venom contains toxin(s) or protein(s) that could be cancer-specific in breast tumor, thus potentiating the venom cytotoxicity at a lower dose in the cancer cells compared to the corresponding normal cells. The cytotoxic effect of Malaysian T. purpureomaculatus venom also appeared to be more potent than other pit viper venoms, including venoms of Protobothrops flavoviridis [35], Trimeresurus macrops and Trimeresurus hageni [36], albeit different human cell lines were utilized in each study.…”

Section: Discussionmentioning

confidence: 96%

Cytotoxic and anticancer properties of the Malaysian mangrove pit viper (Trimeresurus purpureomaculatus) venom and its disintegrin (purpureomaculin)

Tan

Liew

Navanesan

et al. 2020

J. Venom. Anim. Toxins incl. Trop. Dis

View full text Add to dashboard Cite

Background: The Asiatic pit vipers from the Trimeresurus complex are medically important venomous snakes. These pit vipers are often associated with snakebite that leads to fatal coagulopathy and tissue necrosis. The cytotoxic venoms of Trimeresurus spp.; however, hold great potential for the development of peptide-based anticancer drugs. Methods: This study investigated the cytotoxic effect of the venom from Trimeresurus purpureomaculatus, the mangrove pit viper (also known as shore pit viper) which is native in Malaysia, across a panel of human cancer cell lines from breast, lung, colon and prostate as well as the corresponding normal cell lines of each tissue. Results: The venom exhibited dose-dependent cytotoxic activities on all cell lines tested, with median inhibition concentrations (IC 50) ranging from 0.42 to 6.98 µg/mL. The venom has a high selectivity index (SI = 14.54) on breast cancer cell line (MCF7), indicating that it is significantly more cytotoxic toward the cancer than to normal cell lines. Furthermore, the venom was fractionated using C 18 reversed-phase high-performance liquid chromatography and the anticancer effect of each protein fraction was examined. Fraction 1 that contains a hydrophilic low molecular weight (approximately 7.5 kDa) protein was found to be the most cytotoxic and selective toward the breast cancer cell line (MCF7). The protein was identified using liquid chromatography-tandem mass spectrometry as a venom disintegrin, termed purpureomaculin in this study. Conclusion: Taken together, the findings revealed the potent and selective cytotoxicity of a disintegrin protein isolated from the Malaysian T. purpureomaculatus venom and suggested its anticancer potential in drug discovery.

show abstract

Section: Discussionmentioning

confidence: 96%

Cytotoxic and anticancer properties of the Malaysian mangrove pit viper (Trimeresurus purpureomaculatus) venom and its disintegrin (purpureomaculin)

Tan

Liew

Navanesan

et al. 2020

J. Venom. Anim. Toxins incl. Trop. Dis

View full text Add to dashboard Cite

show abstract

“…The search parameters were set including one missed cleavage site, variable modifications of carbamidomethyl (C) and oxidation (M), 0.8 Da for MS peptide tolerance, and 0.8 Da for MS/MS tolerance due to the default parameter setting of the Mascot Daemon 2.2.2 software. Three biological replications were performed [33,34].…”

Section: Proteomic Analysismentioning

confidence: 99%

Biochemical and proteomic analyses of venom from a new pit viper, Protobothrops kelomohy

Chanhome¹,

Khow²,

Reamtong³

et al. 2022

J. Venom. Anim. Toxins incl. Trop. Dis

View full text Add to dashboard Cite

Background: A new pit viper, Protobothrops kelomohy, has been recently discovered in northern and northwestern Thailand. Envenoming by the other Protobothrops species across several Asian countries has been a serious health problem since their venom is highly hematotoxic. However, the management of P. kelomohy bites is required as no specific antivenom is available. This study aimed to investigate the biochemical properties and proteomes of P. kelomohy venom (PKV), including the cross-neutralization to its lethality with antivenoms available in Thailand. Methods: PKV was evaluated for its neutralizing capacity (ER 50 ), lethality (LD 50 ), procoagulant and hemorrhagic effects with three monovalent antivenoms (TAAV, DSAV, and CRAV) and one polyvalent (HPAV) hematotoxic antivenom. The enzymatic activities were examined in comparison with venoms of Trimeresurus albolabris (TAV), Daboia siamensis (DSV), Calloselasma rhodostoma (CRV). Molecular mass was separated on SDS-PAGE, then the specific proteins were determined by western blotting. The venom protein classification was analyzed using mass spectrometry-based proteomics. Results: Intravenous LD 50 of PKV was 0.67 µg/g. ER 50 of HPAV, DSAV and TAAV neutralize PKV at 1.02, 0.36 and 0.12 mg/mL, respectively. PKV exhibited procoagulant effect with a minimal coagulation dose of 12.5 ± 0.016 µg/mL and hemorrhagic effect with a minimal hemorrhagic dose of 1.20 ± 0.71 µg/mouse. HPAV was significantly effective in neutralizing procoagulant and hemorrhagic effects of PKV than those of TAAV, DSAV and CRAV. All enzymatic activities among four venoms exhibited significant differences. PKV proteome revealed eleven classes of putative snake venom proteins, predominantly metalloproteinase (40.85%), serine protease (29.93%), and phospholipase A 2 (15.49%).

show abstract

“…A 30 μg sample of O . monticola venom was separated by 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) (Bio-Rad, USA) and stained by Coomassie R-250 solution (Bio-Rad, USA) as previously described [ 13 ]. The whole lane of venom was excised into 10 pieces and further subjected to in-gel digestion.…”

Section: Methodsmentioning

confidence: 99%

“…A mixture of 50% acetonitrile (ACN) in 50 mM ammonium bicarbonate was used for de-staining the blue color from gel slides [ 13 ]. Venom proteins were reduced by 4mM DTT and incubated at 60°C for 15 min.…”

Section: Methodsmentioning

confidence: 99%

Proteomics and immunocharacterization of Asian mountain pit viper (Ovophis monticola) venom

et al. 2021

Self Cite

View full text Add to dashboard Cite

The venomic profile of Asian mountain pit viper Ovophis monticola is clarified in the present study. Using mass spectrometry-based proteomics, 247 different proteins were identified in crude venom of O. monticola found in Thailand. The most abundant proteins were snake venom metalloproteases (SVMP) (36.8%), snake venom serine proteases (SVSP) (31.1%), and phospholipases A2 (PLA2) (12.1%). Less abundant proteins included L-amino acid oxidase (LAAO) (5.7%), venom nerve growth factor (3.6%), nucleic acid degrading enzymes (3.2%), C-type lectins (CTL) (1.6%), cysteine-rich secretory proteins (CRISP) (1.2%) and disintegrin (1.2%). The immunoreactivity of this viper’s venom to a monovalent antivenom against green pit viper Trimeresurus albolabris, or to a polyvalent antivenom against hemotoxic venom was investigated by indirect ELISA and two-dimensional (2D) immunoblotting. Polyvalent antivenom showed substantially greater reactivity levels than monovalent antivenom. A titer for the monovalent antivenom was over 1:1.28x107 dilution while that of polyvalent antivenom was 1:5.12x107. Of a total of 89 spots comprising 173 proteins, 40 spots of predominantly SVMP, SVSP and PLA2 were specific antigens for antivenoms. The 49 unrecognized spots containing 72 proteins were characterized as non-reactive proteins, and included certain types of CTLs and CRISPs. These neglected venom constituents could limit the effectiveness of antivenom-based therapy currently available for victims of pit viper envenomation.

show abstract

Venomics and Cellular Toxicity of Thai Pit Vipers (Trimeresurus macrops and T. hageni)

Cited by 9 publications

References 45 publications

Cytotoxic and anticancer properties of the Malaysian mangrove pit viper (Trimeresurus purpureomaculatus) venom and its disintegrin (purpureomaculin)

Cytotoxic and anticancer properties of the Malaysian mangrove pit viper (Trimeresurus purpureomaculatus) venom and its disintegrin (purpureomaculin)

Biochemical and proteomic analyses of venom from a new pit viper, Protobothrops kelomohy

Proteomics and immunocharacterization of Asian mountain pit viper (Ovophis monticola) venom

Contact Info

Product

Resources

About