Venous endothelin guides sympathetic innervation of the developing mouse heart

Manousiouthakis, Eleana; Mendez, Monica; Garner, Madeline; Exertier, Prisca; Makita, Takako

doi:10.1038/ncomms4918

Cited by 32 publications

(43 citation statements)

References 26 publications

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“…For whole-mount immunohistochemical analysis, samples were dehydrated by a methanol series and incubated overnight in methanol containing DMSO (20%) and H 2 O 2 (3%) to quench endogenous peroxidase activity [20]. Samples were rehydrated by methanol series and blocked and permeabilised in PBS containing BSA (10% (w/v)) and triton-X100 (1% (w/v)).…”

Section: Methodsmentioning

confidence: 99%

Imaging the mammary gland and mammary tumours in 3D: optical tissue clearing and immunofluorescence methods

et al. 2016

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BackgroundHigh-resolution 3D imaging of intact tissue facilitates cellular and subcellular analyses of complex structures within their native environment. However, difficulties associated with immunolabelling and imaging fluorescent proteins deep within whole organs have restricted their applications to thin sections or processed tissue preparations, precluding comprehensive and rapid 3D visualisation. Several tissue clearing methods have been established to circumvent issues associated with depth of imaging in opaque specimens. The application of these techniques to study the elaborate architecture of the mouse mammary gland has yet to be investigated.MethodsMultiple tissue clearing methods were applied to intact virgin and lactating mammary glands, namely 3D imaging of solvent-cleared organs, see deep brain (seeDB), clear unobstructed brain imaging cocktails (CUBIC) and passive clarity technique. Using confocal, two-photon and light sheet microscopy, their compatibility with whole-mount immunofluorescent labelling and 3D imaging of mammary tissue was examined. In addition, their suitability for the analysis of mouse mammary tumours was also assessed.ResultsVarying degrees of optical transparency, tissue preservation and fluorescent signal conservation were observed between the different clearing methods. SeeDB and CUBIC protocols were considered superior for volumetric fluorescence imaging and whole-mount histochemical staining, respectively. Techniques were compatible with 3D imaging on a variety of platforms, enabling visualisation of mammary ductal and lobulo-alveolar structures at vastly improved depths in cleared tissue.ConclusionsThe utility of whole-organ tissue clearing protocols was assessed in the mouse mammary gland. Most methods utilised affordable and widely available reagents, and were compatible with standard confocal microscopy. These techniques enable high-resolution, 3D imaging and phenotyping of mammary cells and tumours in situ, and will significantly enhance our understanding of both normal and pathological mammary gland development.Electronic supplementary materialThe online version of this article (doi:10.1186/s13058-016-0754-9) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Imaging the mammary gland and mammary tumours in 3D: optical tissue clearing and immunofluorescence methods

et al. 2016

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“…Furthermore, in the outermost layer of larger coronary artery segments is the adventitia, which contains fibroblasts and other cell types (18, 19). Finally, select coronary vessels, particularly arteries and veins, are innervated (20, 21) and associate with lymphatic vessels (22). Interestingly, in humans, larger arteries and veins are located in a subepicardial position, whereas in rodents, arteries are deep within the myocardium (intramyocardial), and veins are on the surface (subepicardial).…”

Section: Overview Of Coronary Vessel Developmentmentioning

confidence: 99%

Coronary Artery Development: Progenitor Cells and Differentiation Pathways

Sharma

Chang

Red‐Horse

2017

Annu. Rev. Physiol.

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Coronary artery disease (CAD) is the number one cause of death worldwide and involves the accumulation of plaques within the artery wall that can occlude blood flow to the heart and cause myocardial infarction. The high mortality associated with CAD makes the development of medical interventions that repair and replace diseased arteries a high priority for the cardiovascular research community. Advancements in arterial regenerative medicine could benefit from a detailed understanding of coronary artery development during embryogenesis and of how these pathways might be reignited during disease. Recent research has advanced our knowledge on how the coronary vasculature is built and revealed unexpected features of progenitor cell deployment that may have implications for organogenesis in general. Here, we highlight these recent findings and discuss how they set the stage to interrogate developmental pathways during injury and disease.

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“…Postganglionic fibers then originate from these ganglia and travel along epicardial vascular structures as dictated by embryological growth cues of endothelin-1 and nerve growth factor (NGF) released by vascular smooth muscle cells, particularly along coronary veins and then arteries. 5,6 Therefore, sympathetic innervation is particularly dense around the sinus node and coronary sinus, with decreasing in density from the base of the ventricle to the apex. 7 In addition, these fibers provide input to the numerous ganglionated subplexuses interspersed throughout bilateral atria and ventricles.…”

Section: Anatomymentioning

confidence: 99%

Cardiac Innervation and the Autonomic Nervous System in Sudden Cardiac Death

Huang¹,

Boyle²,

Vaseghi³

2017

Cardiac Electrophysiology Clinics

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Summary Neural remodeling in the autonomic nervous system contributes significantly to sudden cardiac death. The fabric of cardiac excitability and propagation are controlled by autonomic innervation. Heart disease predisposes to malignant ventricular arrhythmias by causing neural remodeling at the level of the myocardium, the intrinsic cardiac ganglia, extra-cardiac intrathoracic sympathetic ganglia, extra-thoracic ganglia, spinal cord, and the brainstem, as well as the higher centers and the cortex. Therapeutic strategies at each of these levels aim to restore the balance between the sympathetic and parasympathetic branches. Understanding this complex neural network will provide further important therapeutic insights into the treatment of sudden cardiac death.

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Venous endothelin guides sympathetic innervation of the developing mouse heart

Cited by 32 publications

References 26 publications

Imaging the mammary gland and mammary tumours in 3D: optical tissue clearing and immunofluorescence methods

Imaging the mammary gland and mammary tumours in 3D: optical tissue clearing and immunofluorescence methods

Coronary Artery Development: Progenitor Cells and Differentiation Pathways

Cardiac Innervation and the Autonomic Nervous System in Sudden Cardiac Death

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