Ventosimonas gracilis gen. nov., sp. nov., a member of the Gammaproteobacteria isolated from Cephalotes varians ant guts representing a new family, Ventosimonadaceae fam. nov., within the order ‘Pseudomonadales’

Lin, Jia; Hobson, William J.; Wertz, John T.

doi:10.1099/ijsem.0.001068

Cited by 18 publications

(8 citation statements)

References 29 publications

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“…These symbiont lineages nest within described orders from the Proteobacteria, Verrucomicrobia, and Bacteroidetes. While most symbionts await formal taxonomic descriptions, work on two core lineages shows that some comprise novel genera 38 and, in at least one case, a novel family 39 .…”

Section: Resultsmentioning

confidence: 99%

Herbivorous turtle ants obtain essential nutrients from a conserved nitrogen-recycling gut microbiome

et al. 2018

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Nitrogen acquisition is a major challenge for herbivorous animals, and the repeated origins of herbivory across the ants have raised expectations that nutritional symbionts have shaped their diversification. Direct evidence for N provisioning by internally housed symbionts is rare in animals; among the ants, it has been documented for just one lineage. In this study we dissect functional contributions by bacteria from a conserved, multi-partite gut symbiosis in herbivorous Cephalotes ants through in vivo experiments, metagenomics, and in vitro assays. Gut bacteria recycle urea, and likely uric acid, using recycled N to synthesize essential amino acids that are acquired by hosts in substantial quantities. Specialized core symbionts of 17 studied Cephalotes species encode the pathways directing these activities, and several recycle N in vitro. These findings point to a highly efficient N economy, and a nutritional mutualism preserved for millions of years through the derived behaviors and gut anatomy of Cephalotes ants.

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Section: Resultsmentioning

confidence: 99%

Herbivorous turtle ants obtain essential nutrients from a conserved nitrogen-recycling gut microbiome

et al. 2018

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“…Moreover, it has been revealed that this bacterium is resistant to the L. sericata larval excreta/secreta (Jaklic et al, 2008). Recently, a member of the Gammaproteobacteria, Ventosimonas gracilis, has been isolated from Cephalotes varians ant guts, which represent a new family, genus, and species (Lin et al, 2016). In our study, the DNA of this obligate aerobic bacterium was found in female flies.…”

Section: Discussionmentioning

confidence: 57%

New Insights Into Culturable and Unculturable Bacteria Across the Life History of Medicinal Maggots Lucilia sericata (Meigen) (Diptera: Calliphoridae)

et al. 2020

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Because of the nutritional ecology of dung-and carrion-feeding, bacteria are the integral part of Lucilia sericata life cycle. Nevertheless, the disinfected larvae of the blowfly are applied to treat human chronic wounds in a biosurgery named maggot debridement therapy (MDT). To realize the effects of location/diet on the gut bacteria, to infer the role of bacteria in the blowfly ecology plus in the MDT process, and to disclose bacteria circulating horizontally in and vertically between generations, bacterial communities associated with L. sericata specimens from various sources were investigated using culture-based and culture-independent methods. In total, 265 bacteria, including 20 families, 28 genera, and 40 species, were identified in many sources of the L. sericata. Culture-dependent method identified a number of 144 bacterial isolates, including 21 species, in flies reared in an insectary; specimens were collected from the field, and thirdinstar larvae retrieved from chronic wounds of patients. Metagenetic approach exposed the occurrences of 121 operational taxonomic units comprising of 32 bacterial species from immature and adult stages of L. sericata. Gammaproteobacteria was distinguished as the dominant class of bacteria by both methods. Bacteria came into the life cycle of L. sericata over the foods and transovarially infected eggs. Enterococcus faecalis, Myroides phaeus, Proteus species, Providencia vermicola, and Serratia marcescens were exchanged among individuals via transstadial transmission. Factors, including diets, feeding status, identification tool, gut compartment, and life stage, governed the bacteria species. Herein, we reemphasized that L. sericata is thoroughly connected to the bacteria both in numerous gut compartments and in different life stages. Among all, transstadially transmitted bacteria are underlined, indicating the lack of antagonistic effect of the larval excretions/secretions on these resident bacteria. While the culture-dependent method generated useful data on the viable aerobic gut bacteria,

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“…In this study, we proved that two novel families, Marinobacteraceae and Perlucidibacaceae ; families Alcanivoracaceae ( 40 ), Balneatrichaceae , Halomonadaceae ( 41 ), Hahellaceae ( 42 ), Oleiphilaceae ( 33 ), Oceanospirillaceae , Saccharospirillaceae ( 43 ), and Zooshikellaceae of order Oceanospirillales ; Pseudomonadaceae and Ventosimonadaceae ( 28 ) of order Pseudomonadales ; and Cellvibrionaceae , Halieaceae , Microbulbiferaceae , Porticoccaceae , and Spongiibacteraceae ( 5 ) of order Cellvibrionales shared a relatively recent ancestor and formed a robust branch based on two typical phylogenomic tree and AAI values in class Gammaproteobacteria . The family Litoricolaceae should also be classified into order Pseudomonadales tentatively because it formed a stable clade with family Saccharospirillaceae (bootstrap value 72) based on the 16S rRNA tree, as no genome is available for Litoricolaceae .…”

Section: Discussionmentioning

confidence: 72%

“…Additionally, the phenotypic characteristics between family Moraxellaceae and other families of order Pseudomonadales are notably different. First, almost all members of family Moraxellaceae contain C 18:1 ω 9 c as a major fatty acid component ( 26 , 27 ), while the component was not detected in other families of Pseudomonadales ( 28 ). Second, the cell shapes of family Moraxellaceae are short rods or coccoid or coccal or may exhibit a characteristic multicellular micromorphology, and cells usually occur in pairs or short chains ( 29 ); however, other families of order Pseudomonadales have just one cell form that is rod-shaped, and cells usually occur in singles ( Table 1 ).…”

Section: Resultsmentioning

confidence: 99%

Reclassification of the Taxonomic Framework of OrdersCellvibrionales, Oceanospirillales, Pseudomonadales, andAlteromonadalesin ClassGammaproteobacteriathrough Phylogenomic Tree Analysis

Liao

Lin

et al. 2020

mSystems

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Orders Oceanospirillales and Pseudomonadales play important roles in various ecosystems as the keystone taxa of microbiomes. However, the two orders present a close evolutionary relationship, which might have caused taxonomic misinterpretation and resulted in an incorrect understanding of their evolutionary history. In this study, first, we used the 16S rRNA gene sequences of 2,049 species of Gammaproteobacteria to build a phylogenetic tree, which demonstrated that reports regarding the evolutionary relationship of orders Cellvibrionales, Oceanospirillales, and Pseudomonadales based on a single conserved gene with a poor resolution have been conflicting; in particular, the major families Moraxellaceae and Pseudomonadaceae of order Pseudomonadales were separated from orders Cellvibrionales and Oceanospirillales. Subsequently, we constructed the bac120 trees of all representative reference genomes of class Gammaproteobacteria based on 120 ubiquitous single-copy proteins from bacteria and a phylogenomic tree based on the 119 core genes of 257 reference genomes obtained from orders Cellvibrionales, Oceanospirillales, and Pseudomonadales to cross validate and infer their intrinsic evolutionary relationships. These results indicated that two novel orders, Moraxellales ord. nov. and Kangiellales ord. nov., and three novel families, Marinobacteraceae fam. nov., Perlucidibacaceae fam. nov., and Zooshikellaceae fam. nov., should be proposed. Additionally, orders Cellvibrionales and Oceanospirillales were merged into the order Pseudomonadales except for families Moraxellaceae and Kangiellaceae in class Gammaproteobacteria, which currently includes 18 families. Our work sheds some light on the evolutionary history of class Gammaproteobacteria, which could facilitate the detection and taxonomic analysis of natural communities. IMPORTANCE The orders Cellvibrionales, Oceanospirillales, and Pseudomonadales, as three major orders of the largest bacterial class, Gammaproteobacteria, play important roles in various ecosystems as the keystone taxa of microbiomes, but their evolutionary relationship is currently polyphyletic and chaotic. Here, we constructed a bac120 tree and core-genome tree and calculated the amino acid identity (AAI) value to explore their intrinsic evolutionary history. In this study, we proposed two novel orders and three novel families. This evolution study vastly reconstructed the taxonomic framework of class Gammaproteobacteria and could provide a more distinct perspective on global distribution and evolutionary patterns of these environmental microorganisms.

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Ventosimonas gracilis gen. nov., sp. nov., a member of the Gammaproteobacteria isolated from Cephalotes varians ant guts representing a new family, Ventosimonadaceae fam. nov., within the order ‘Pseudomonadales’

Cited by 18 publications

References 29 publications

Herbivorous turtle ants obtain essential nutrients from a conserved nitrogen-recycling gut microbiome

Herbivorous turtle ants obtain essential nutrients from a conserved nitrogen-recycling gut microbiome

New Insights Into Culturable and Unculturable Bacteria Across the Life History of Medicinal Maggots Lucilia sericata (Meigen) (Diptera: Calliphoridae)

Reclassification of the Taxonomic Framework of OrdersCellvibrionales, Oceanospirillales, Pseudomonadales, andAlteromonadalesin ClassGammaproteobacteriathrough Phylogenomic Tree Analysis

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