Ventricular remodelling in rabbits with sustained high‐fat diet

Zarzoso, Manuel; Mironov, Sergey V.; Guerrero-Serna, Guadalupe; Willis, B. Cicero; Pandit, Sandeep V.

doi:10.1111/apha.12185

Cited by 25 publications

(21 citation statements)

References 49 publications

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“…We observed a 24 and 22% increase in bodyweight and BMI, respectively, at week 28, showing that rabbits were obese but they did not reach a degree of severe obesity. This is a similar increase in weight obtained in previous studies with younger male rabbits [11] fed with high-fat diet, but far from the 45% increase obtained by Carroll et al . [21] in adult female rabbits.…”

Section: Discussionsupporting

confidence: 90%

“…Rabbits, as herbivores, are very sensitive to dietary fat, therefore, they can rapidly develop hyperlipidemia [24]. In addition, it has been shown that rabbits fed with a high-fat diet have similar hemodynamic and neurohumoral changes as obese humans [21,11]. …”

Section: Discussionmentioning

confidence: 99%

“…For the evaluation of glucose metabolism, intravenous glucose tolerance test (IVGTT) was performed as previously described [11]. Briefly, rabbits were fasted during 7 hours and then the experiment started between 14 and 15 p.m. After auricular vein cannulation, a bolus of a 60% glucose solution (0.6 g kg -1 ) was administered i.v.…”

Section: Methodsmentioning

confidence: 99%

“…On the other hand, in the rabbit ventricular myocardium, the action potential duration is approximately 200–300 ms, the morphology is spike and dome shaped, and the repolarization is mainly mediated by I Kr and I Ks , which is similar to the human ventricle [9]. In addition, the rabbit model has been widely used for studying sustained arrhythmias and ventricular fibrillation [10,11], which is the main cause of SCD.…”

Section: Introductionmentioning

confidence: 99%

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Development and characterization of an experimental model of diet-induced metabolic syndrome in rabbit

et al. 2017

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Metabolic syndrome (MetS) has become one of the main concerns for public health because of its link to cardiovascular disease. Murine models have been used to study the effect of MetS on the cardiovascular system, but they have limitations for studying cardiac electrophysiology. In contrast, the rabbit cardiac electrophysiology is similar to human, but a detailed characterization of the different components of MetS in this animal is still needed. Our objective was to develop and characterize a diet-induced experimental model of MetS that allows the study of cardiovascular remodeling and arrhythmogenesis. Male NZW rabbits were assigned to control (n = 15) or MetS group (n = 16), fed during 28 weeks with high-fat, high-sucrose diet. We measured weight, morphological characteristics, blood pressure, glycaemia, standard plasma biochemistry and the metabolomic profile at weeks 14 and 28. Liver histological changes were evaluated using hematoxylin-eosin staining. A mixed model ANOVA or unpaired t-test were used for statistical analysis (P<0.05). Weight, abdominal contour, body mass index, systolic, diastolic and mean arterial pressure increased in the MetS group at weeks 14 and 28. Glucose, triglycerides, LDL, GOT-AST, GOT/GPT, bilirubin and bile acid increased, whereas HDL decreased in the MetS group at weeks 14 and 28. We found a 40% increase in hepatocyte area and lipid vacuoles infiltration in the liver from MetS rabbits. Metabolomic analysis revealed differences in metabolites related to fatty acids, energetic metabolism and microbiota, compounds linked with cardiovascular disease. Administration of high-fat and high-sucrose diet during 28 weeks induced obesity, glucose intolerance, hypertension, non-alcoholic hepatic steatosis and metabolic alterations, thus reproducing the main clinical manifestations of the metabolic syndrome in humans. This experimental model should provide a valuable tool for studies into the mechanisms of cardiovascular problems related to MetS, with special relevance in the study of cardiovascular remodeling, arrhythmias and SCD.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Development and characterization of an experimental model of diet-induced metabolic syndrome in rabbit

et al. 2017

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“…In contrast, high‐fat feeding induced left ventricular hypertrophy and a type II diabetes phenotype in rabbits (Zarzoso et al . ).…”

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confidence: 97%

High expectations: from treatment to prevention

Ludwig

Högner

2015

Acta Physiologica

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Multiple time‐dependent pathophysiological changes in a rabbit model of high‐fat diet‐induced hyperlipidemia

et al. 2023

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High‐fat diets (HFD) adversely affect organ systems. Several studies have examined HFD‐related disorders in animals but only in a few organs and time points. Herein, we evaluated disease development with time‐dependent HFD‐induced pathological, cardiovascular, and morphological changes in rabbits with lipid metabolism similar to that in humans for 9 weeks. The body weights and waist ratio of the HFD group were higher than those in the control group. HFD significantly increased the total cholesterol, low‐density lipoprotein, high‐density lipoprotein, and phospholipid levels after 3 weeks. Liver enzyme levels increased with hepatomegaly, steatosis, and fibrosis after 3 or 6 weeks. RBCs and hemoglobin decreased, while platelets increased in the HFD group with atherosclerosis and inflammatory cell infiltration in the aorta after 6 weeks. Ejection fraction and fractional shortening values decreased in the HFD group after 9 weeks. Creatinine increased with glomerulosclerosis in the kidneys of the HFD groups after 3 weeks, indicating renal dysfunction. Lipid accumulation was found in the pancreas after 9 weeks. Lipid accumulation and hypertrophy were observed in the adrenal glands after 3 weeks. Overall, our findings provide global reference data on the time‐dependent effects of HFD on the body and may serve as a guide for future HFD risk prevention.

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Ventricular remodelling in rabbits with sustained high‐fat diet

Abstract: High-fat feeding during 18 weeks in rabbits induced a type II diabetes phenotype, LV hypertrophy, abnormalities in repolarization and susceptibility to arrhythmias during hyperkalaemia.

Cited by 25 publications

References 49 publications

Development and characterization of an experimental model of diet-induced metabolic syndrome in rabbit

Development and characterization of an experimental model of diet-induced metabolic syndrome in rabbit

High expectations: from treatment to prevention

Multiple time‐dependent pathophysiological changes in a rabbit model of high‐fat diet‐induced hyperlipidemia

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