Veränderungen des Enzymmusters in primären Gewebekulturen

Korfsmeier, K. H.

doi:10.1007/bf00306032

Histochemie

1972

DOI: 10.1007/bf00306032

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Veränderungen des Enzymmusters in primären Gewebekulturen

K. H. Korfsmeier

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1975

1988

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Cited by 5 publications

References 44 publications

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Zur Histochemie der Flimmerzellen der menschlichen Endosalpinx

Kügler

1981

Histochemistry

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The human endosalpingeal ciliated cells are not a homogeneous cell population. They can be distinguished into mitochondria prominent and normal cells. The morphological appearance of ciliated cells was studied in organ culture using different hormones and hormone combinations in the culture media. Histological (HE, semithin sections) and histochemical methods (PAS, Alcian Blue, SDH, LDH, ATPase, 5'-Nucleotidase, acid and alkaline Phosphatase) were applied. Those mitochondria prominent ciliated cells which are seen in the native endosalpinx can in vitro also be determined mainly under the influence of steroid hormones (hydrocortisone and progesterone). In hormone free incubation media the apices of ciliated cells are separated. This will happen in hormone containing media, too, but with delay, however. Some results are in agreement with the possible transformation from ciliated cells to secretory cells.

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Zur Histochemie der Flimmerzellen der menschlichen Endosalpinx

Kügler

1981

Histochemistry

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Localization of acid phosphatase activity in collagen-secreting and collagen-resorbing fibroblasts

Yajima

1988

Histochemistry

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The ultrastructural localization of acid phosphatase (ACPase) activity was examined in cultured human gingival fibroblasts in the formative and resorptive phases. In the collagen-secreting fibroblasts, weak ACPase activity was demonstrated in the lysosomes, inner Golgi cisternae, and condensing vacuoles, and none was found in the Golgi-associated endoplasmic reticulum-lysosome system (GERL), presecretory granules, or secretory granules. On the contrary, collagen phagocytosis induced strong ACPase activity in the GERL, which was in addition to the weaker activity found in the same sites as those in the collagen-secreting cells. At the same time, collagen secretion was suppressed, and dense elongated secretory bodies associated with ACPase activity accumulated within the cells. When collagen fibrils had been interiorized in whole or in part within the phagosomes, primary lysosomes derived from the Golgi-GERL complex then fused with them to form phagolysosomes. Collagen degradation occurred within these bodies. The observations indicate significant differences in ACPase activity used as a marker for lysosomal enzyme activities in the different functional phases of fibroblasts. These results suggest that fibroblasts work only one way at a given time, viz., collagen synthesis or collagen degradation.

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Histochemical findings in different types of malignant melanoma: Biological and clinical significance

Paul

1975

Arch. Derm. Res.

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By means of histochemical methods (gel-film incubation-media) superficial spreading melanoma, nodular melanoma and lentigo maligna melanoma are investigated. The result of this examination is that with regard to their enzyme spectra, the nodular melanoma and the nodular part of the superficial spreading melanoma are very similar. Glucose-6-phosphate dehydrogenase shows the strongest enzyme reaction, followed by succinate dehydrogenase and lactate dehydrogenase. The beta-hydroxybutyrate dehydrogenase reaction is always weak. The reaction of acid phosphatase is between negative and weakly positive. Significant differences, however, are observed in lentigo maligna and in lentigo maligna melanoma. In both, the strongest formazan deposits are seen with succinate dehydrogenase, sometimes also with lactate dehydrogenase. The glucose-6-phosphate dehydrogenase reaction, however, is sometimes considerably weaker. In the case of lentigo maligna melanoma, the activity of beta-hydroxybutyrate dehydrogenase often is increased, and acid phosphatase also shows higher reactions than in the other melanomas. These differences in the enzyme pattern correspond to the different biological behavior of the tumours. The enzymatical and biological characteristics of lentigo maligna melanoma possibly derive more from the characteristics of the tumour itself which are not dependent on the area.

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Veränderungen des Enzymmusters in primären Gewebekulturen

Cited by 5 publications

References 44 publications

Zur Histochemie der Flimmerzellen der menschlichen Endosalpinx

Zur Histochemie der Flimmerzellen der menschlichen Endosalpinx

Localization of acid phosphatase activity in collagen-secreting and collagen-resorbing fibroblasts

Histochemical findings in different types of malignant melanoma: Biological and clinical significance

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