Verbascoside attenuates experimental varicocele-induced damage to testes and sperm levels through up-regulation of the hypothalamus-pituitary-gonadal (HPG) axis

Han, Letian; Xiang, Shufen; Rong, Baohai; Liang, Yanchen; Zhao, Shengtian

doi:10.1080/13880209.2021.1933085

Cited by 7 publications

(3 citation statements)

References 38 publications

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“…AC can significantly enhance sperm viability, increase Johnson’s score, and decrease apoptotic index. These might be associated with up regulation of the hypothalamus-pituitary-gonadal axis by AC [150] . AC exhibits anti-estrogenic effects in ERα- and Erβ-transfected HeLa cells.…”

Section: Miscellaneous Sectionmentioning

confidence: 99%

The pharmacokinetic property and pharmacological activity of acteoside: A review

Xiao¹,

Ren²,

Wu³

2022

Biomedicine & Pharmacotherapy

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Section: Miscellaneous Sectionmentioning

confidence: 99%

The pharmacokinetic property and pharmacological activity of acteoside: A review

Xiao¹,

Ren²,

Wu³

2022

Biomedicine & Pharmacotherapy

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“…Varicocele is considered to be a cause of male infertility, able to induce abnormal semen with decreased motility of spermatozoa, low sperm count, and abnormal structure of the seminiferous epithelium [10,11,20]. Recent evidence suggested the pathophysiologic mechanisms of the disease, demonstrating that many factors, such as increased scrotal temperature, hypoxia, oxidative stress, augmented inflammatory cytokines, apoptosis, and hormonal imbalance are involved.…”

Section: Discussionmentioning

confidence: 99%

Positive Effects of the Nutraceutical Association of Lycopene and Selenium in Experimental Varicocele

Freni,

Pallio,

Marini

et al. 2023

IJMS

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Many natural substances commonly found in healthy diets have been studied for their potential to reduce male infertility associated with varicocele. A positive role of selenium (Se) or lycopene alone was demonstrated in experimental varicocele, while no data are available on their association. One group of male Sprague–Dawley rats was sham operated and daily treated with Se (3 mg/kg, i.p.), lycopene (1 mg/kg, i.p.), or their association. A second group underwent surgery to induce varicocele. Sham and half of the varicocele animals were sacrificed after twenty-eight days, while the residual animals were treated for one more month and then sacrificed. In varicocele animals, testosterone levels and testes weight were reduced, Hypoxia Inducible Factor-1α (HIF-1α) expression was absent in the tubules and increased in Leydig cells, caspare-3 was increased, seminiferous epithelium showed evident structural changes, and many apoptotic germ cells were demonstrated with TUNEL assay. The treatment with lycopene or Se alone significantly increased testis weight and testosterone levels, reduced apoptosis and caspase-3 expression, improved the tubular organization, decreased HIF-1α positivity of Leydig cells, and restored its tubular positivity. Lycopene or Se association showed a better influence on all biochemical and morphological parameters. Therefore, the nutraceutical association of lycopene plus Se might be considered a possible therapeutic tool, together with surgery, in the treatment of male infertility. However, long-term experimental and clinical studies are necessary to evaluate sperm quantity and quality.

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“…Additionally, salicin and salvianolic acid B can protect mitochondrial function and inhibit the mitochondrial apoptosis pathway; however, its effects on spermatogenic cells and sperm vitality have not been widely examined (Song et al, 2019; Zheng et al, 2020). Verbascoside has protective effects on reproductive organs, significantly increases sperm viability, and reduces the AI (Han et al, 2021). Finally, Lycium barbarum polysaccharide elicits an apparent protective effect on the reproductive and spermatogenic functions of male rats and can significantly reduce spermatogenic cell apoptosis by upregulating Bcl‐2 while downregulating Bax (Luo et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Jujing Zhuyu decoction inhibits apoptosis in rats with asthenozoospermia by regulating the mitochondrial apoptosis pathway

Lin

et al. 2022

Andrologia

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Jujing Zhuyu decoction (JZD) is a traditional Chinese medicine that effectively improves sperm motility. However, the molecular mechanism of JZD on asthenozoospermia still remains unknown. In this study, we investigated the effect of JZD on the mitochondrial apoptosis pathway in an asthenozoospermia rat model. Sixty Sprague–Dawley rats were randomly divided into five groups—control, tripterygium glycosides (GTW) model, JZD‐low (JZD‐L), JZD‐medium (JZD‐M), and JZD‐high (JZD‐H) groups (n = 12/group). GTW was used to generate the asthenozoospermia model. The JZD‐L, JZD‐M, and JZD‐H groups were administered 5, 10, or 15 g kg−1 day−1 of JZD granules respectively, for 4 weeks. Testicular tissue morphology was examined using histological staining, while sperm count was determined using manual and computer‐aided semen analyses. Apoptosis of spermatogenic cells was detected with the TUNEL assay, and the expression of proteins and genes related to mitochondrial apoptosis was detected using western blotting and quantitative reverse transcription–polymerase chain reaction respectively. Histomorphological evaluation revealed superior seminiferous tubule structure and arrangement as well as improved spermatogenic cell morphology in the JZD‐L, JZD‐M, and JZD‐H groups compared to those in the model group. Moreover, semen quality and the apoptotic index were significantly improved in the JZD‐L, JZD‐M, and JZD‐H groups compared to those in the model group. Additionally, the mRNA expression and protein abundance of Apaf‐1, Bax, Cyto‐c, and caspase‐3 was reduced, while those of Bcl‐2 were increased in all JZD groups compared to those in the model group. JZD reduces the apoptosis rate of sperm cells and significantly promotes sperm survival by regulating the mitochondrial apoptosis pathway. This mechanism provides experimental support for the treatment of asthenozoospermia by JZD.

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Verbascoside attenuates experimental varicocele-induced damage to testes and sperm levels through up-regulation of the hypothalamus-pituitary-gonadal (HPG) axis

Abstract: 2021) Verbascoside attenuates experimental varicocele-induced damage to testes and sperm levels through up-regulation of the hypothalamus-pituitary-gonadal (HPG) axis,

Cited by 7 publications

References 38 publications

The pharmacokinetic property and pharmacological activity of acteoside: A review

The pharmacokinetic property and pharmacological activity of acteoside: A review

Positive Effects of the Nutraceutical Association of Lycopene and Selenium in Experimental Varicocele

Jujing Zhuyu decoction inhibits apoptosis in rats with asthenozoospermia by regulating the mitochondrial apoptosis pathway

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