Verbascoside enhances radiosensitivity of hepatocellular carcinoma cells through regulating miR‐101‐3p/Wee1 axis

Abstract

Verbascoside is a kind of phenylpropanoid glycoside derived from multiple medicinal plants, exerting anti-tumor effects in diverse human malignancies. However, the function of Verbascoside on the radiosensitivity of hepatocellular carcinoma (HCC) cells remains unknown. Human Huh7 and HepG2 cell lines were treated with Verbascosideis, and cell viability was detected with cell counting kit-8 (CCK-8) assay. Quantitative real-time polymerase chain reaction (qRT-PCR) was applied to detect miR-101-3p expression, and Western blot was used to quantify the expression of WEE1 G2 checkpoint kinase (WEE1). Then, CCK-8 and flow cytometry assays were used to detect the proliferation and apoptosis of HCC cells after Verbascoside and X-ray combined treatment, and the expressions of WEE1 and apoptosis-related proteins Bax and Bcl-2 were detected by Western blot. Verbascoside could improve the radiosensitivity of HCC cells in a dosedependent manner. Verbascoside increased the expression of miR-101-3p but reduced WEE1 expression in HCC cells. Additionally, WEE1 was identified as a target of miR-101-3p. MiR-101-3p inhibition or WEE1 overexpression could reverse the effect of Verbascoside on the viability and apoptosis of HCC cells. Verbascoside increases the radiosensitivity of hepatocellular carcinoma cells via modulating miR-101-3p/WEE1 axis.

Rho GTPase‐activating protein 1 promotes hepatocellular carcinoma progression via modulation by CircPIP5K1A/MiR‐101‐3p

“…For example, microRNA (miRNA) induced gene repression by binding to 3′‐untranslational regions (3′UTR) of target mRNA. In HCC, a reduction in miR‐101‐3p expression has been reported, together with its association with the proliferation of liver cancer cells 13–16 . MiR‐101‐3p was a key tumor‐related gene with potential preclinical development applications.…”

“…In HCC, a reduction in miR-101-3p expression has been reported, together with its association with the proliferation of liver cancer cells. [13][14][15][16] MiR-101-3p was a key tumor-related gene with potential preclinical development applications. Meanwhile, it is suggested that ARHGAP1 is a potential post-transcriptional target of cancer-related miRNAs.…”

miR-101-3p-mediated role of PDZK1 in hepatocellular carcinoma progression and the underlying PI3K/Akt signaling mechanism

“…In serous ovarian cancer, miR-101-3p was found to inhibit epithelial–mesenchymal transition and tumor invasion and metastasis by regulating the PTAR/ZBE1 axis [ 25 ]. miR-101-3p has also been found to be involved in HCC progression and radiosensitivity [ 26 , 27 ]. However, there is very little research about the interaction between PDZK1 and miR-101-3p in HCC.…”