Verden i et sandskorn

Møller, Marie Sofie

doi:10.7146/kok.v29i92.21082

Cited by 1 publication

(1 citation statement)

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“…Unlike the MVLN cell bioassay, the ( -)-a-HCH was found be significantly more potent than the (+)-a-HCH in inhibition rate of ER-b binding affinity assay below a concentration of 100 lM. The significant difference between (+)-a-HCH and ( -)-a-HCH was reported concerning the cytotoxic effect as well as the growth stimulation of mitosis in primary rat hepatocytes [10]. Thus, the quantitative determination of a-HCH as racemic mixture may lead to an overestimation or underestimation of the toxicity of this compound and the enantioselective determination of the potencies of a-HCH may be essential for the study of the biological effects on organisms.…”

Section: Estrogen Receptor-b Inhibition Ratesmentioning

confidence: 74%

An automated enantioselective isolation system for the study of estrogenic potencies: Study of the estrogenic activity of α‐hexachlorocyclohexane

Hashimoto¹,

Yoshimoto

Nakao

et al. 2003

J of Separation Science

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An automated enantioselective isolation system for the study of estrogenic potencies: Study of the estrogenic activity of a-hexachlorocyclohexane Herein we describe an automated enantioselective isolation system that uses a gas chromatograph-flame ionization detector (FID)-preparative fraction collector to separate the enantiomers of chiral compounds. In this system, a switching device was coupled with six glass tubes containing an organic solvent for the separate collection of six compounds, thus eliminating the need for a manual collection procedure after separation by gas chromatography. To evaluate the usefulness of this system, we applied it to the separation and collection of the enantiomers of a-hexachlorocyclohexane (a-HCH), and we measured the estrogenic potency of each enantiomer by means of an MVLN cell (Michigan Cancer Foundation-7 (MCF-7) cell stably transfected with a pVit-tk-LUC reporter plasmid) bioassay and an estrogen receptor binding assay. The variability in the retention time of each enantiomer (relative standard deviation, n = 16) was typically a 0.05%. The recoveries of the dextrorotatory (+) and levorotatory ( -) enantiomers of a-HCH were about 68.8% and 73.4%, respectively. The overall quantity of each enantiomer of a-HCH collected was about 300 lg. Both enantiomers of a-HCH showed similar estrogenic activity. On the basis of the results obtained for a-HCH, we concluded that this analytical method is suitable for the determination of the estrogenic activity of other chiral compounds. Such determinations are essential because enantiomers differ considerably in how they accumulate in organisms and decompose in the environment.

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Section: Estrogen Receptor-b Inhibition Ratesmentioning

confidence: 74%