Vereinfachte Methode Der Blutzuckerbestimmung

Jendrassik, L.; Polgar, Alexander M.

doi:10.1007/bf02303998

Cited by 3 publications

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The serum activity of acetylcholinesterase (AChE) [ 17 ], total protein [ 18 ], alkaline phosphatase [ 19 ], aspartate aminotransferase (AST) and alanine aminotransferase (ALT) [ 20 ], glucose [ 21 ], urea [ 22 ], creatinin [ 23 ], and bilirubin [ 24 ] were estimated as described.…”

Section: Acute Oral and Injection Toxicity Studies Of Acaait Acmnpv And Slnpv In Albino Ratsmentioning

confidence: 99%

Biosafety of Recombinant and Wild Type Nucleopolyhedroviruses as Bioinsecticides

Ashour

Ragheb

El-Sheikh

et al. 2007

IJERPH

View full text Add to dashboard Cite

The entomopathogenic Autographa californica (Speyer) nucleopolyhedrovirus (AcMNPV) has been genetically modified to increase its speed of kill. The potential adverse effects of a recombinant AcMNPV (AcAaIT) as well as wild type AcMNPV and wild type Spodoptera littoralis NPV (SlNPV) were studied. Cotton plants were treated with these viruses at concentrations that were adjusted to resemble the recommended field application rate (4 × 1012 PIBs/feddan, feddan = 4,200 m2) and 3rd instar larvae of S. littoralis were allowed to feed on the contaminated plants. SDS-PAGE, ELISA, and DNA analyses were used to confirm that larvae that fed on these plants were virus-infected. Polyhedra that were purified from the infected larvae were subjected to structural protein analysis. A 32 KDa protein was found in polyhedra that were isolated from all of the viruses. Subtle differences were found in the size and abundance of ODV proteins. Antisera against polyhedral proteins isolated from AcAaIT polyhedra were raised in rabbits. The terminal bleeds from rabbits were screened against four coating antigens (i.e., polyhedral proteins from AcAaIT, AcAaIT from field-infected larvae (AcAaIT-field), AcMNPV, and SlNPV) using a two-dimensional titration method with the coated antigen format. Competitive inhibition experiments were conducted in parallel to optimize antibody and coating antigen concentrations for ELISA. The IC50 values for each combination ranged from 1.42 to 163 μg/ml. AcAaIT-derived polyhedrin gave the lowest IC50 value, followed by those of SlNPV, AcAaIT-field, and AcMNPV. The optimized ELISA system showed low cross reactivity for AcMNPV (0.87%), AcAaIT-field (1.2%), and SlNPV (4.0%). Genomic DNAs isolated from AcAaIT that were passaged in larvae of S. littoralis that were reared in the laboratory or field did not show any detectable differences. Albino rats (male and female) that were treated with AcAaIT, AcMNPV or SlNPV (either orally or by intraperitoneal injection at doses of 1 × 108 or 1 × 107 PIBs/rat, respectively) appeared to be healthy and showed increased body weight at 21 days posttreatment. The effect of virus administration on hematological, serum biochemical, and histopathological parameters were determined. Slight to moderate differences were observed in most of the hematological parameters. Specifically, serum proteins were decreased markedly in female rats treated orally with SlNPV, and in male rats injected with AcAaIT. SDS-PAGE analysis also showed some changes in serum protein profiles. No marked changes in acetylcholine esterase (AChE) activity were found. Changes in serum glucose, alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), bilirubin, creatinin, and urea were also observed. Immunohistochemical observation of tissues from stomach, intestine, liver, kidney, brain, spleen, and lung also showed slight changes. Fish (Tilapia nilotica) were also exposed to AcAaIT, AcMNPV or SlNPV by incorporating each of the viruses into diet (1 × 109 PIBs/group). No mortality...

show abstract

Section: Acute Oral and Injection Toxicity Studies Of Acaait Acmnpv And Slnpv In Albino Ratsmentioning

confidence: 99%

Biosafety of Recombinant and Wild Type Nucleopolyhedroviruses as Bioinsecticides

Ashour

Ragheb

El-Sheikh

et al. 2007

IJERPH

View full text Add to dashboard Cite

show abstract

“…Most methods for determining bilirubin are still based on the diazo reaction. Fog (1958) has produced a modification of the Jendrassik and Gr6f (1938) procedure by reading the diazo pigment in alkali at 660 mIL. This reduces the effect of other pigments.…”

Section: Methodsmentioning

confidence: 99%

Changing Perspectives on Bilirubin

Lathe

1964

Proceedings of the Association of Clinical Biochemists

View full text Add to dashboard Cite

“…Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured using ALT and AST diagnostic kit according to International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) without pyridoxal phosphate activation [19,20]. The absorbance was read at 340nm against sample blank which automatically recorded the concentration of ALT and AST in international unit (U/L).…”

mentioning

confidence: 99%

“…The absorbance was read at 340nm against sample blank which automatically recorded the concentration of ALT and AST in international unit (U/L). Creatinine was measured with creatinine diagnostic kit using modified Jaffe method [19] . The absorbance was measured at 510nm and creatinine concentration was given in µmol/L [3,4] .…”

mentioning

confidence: 99%

Effect of dihydroartemisinin and its disulphidederivative on biochemical, histological and hematological parameters in rat

Etim

Udoh

Udobang

et al. 2018

IJMSCI

View full text Add to dashboard Cite

Abstract:Introduction: The disulphide derivative of dihydroartemisinin was synthesized and confirmed of being effective against Plasmodium berghei berghei, some gramme positive bacteria and fungi. Objective: The study was designed to compare the effect of the synthesized disulphide derivative of dihydroartemisinin with pure dihydroartemisinin on biochemical, histological and hematological parameters. Method: Doses of 10%, 20% and 30% of the LD 50 of pure dihydroartemisinin and its disulphide derivatives were orally administered to each group of rat for 14 days. On the 14 th day, the rats were sacrificed, and blood collected by cardiac puncture was used for hematological and biochemical studies. The liver and kidney were removed, weighed and preserved in 10% formalin for histopathology studies. Results: Using Lork's method, the LD 50 for pure dihydroartemisinin was 547.72 mg/kg while the disulfide derivative was 346.41mg/kg. There was a significant difference (p<0.05) in the concentrations of hematological parameters in treated rats from those of the control group. There was a dose dependent increase in the levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in the treated groups. The cells of the liver and kidney were slightly affected by all the doses of dihydroartemisinin and moderately by the disulphide derivative. Conclusion: The toxicity of dihydroartemisinin (DHA) and its disulphide derivative (SDHA) are almost the same, making the SDHA not too toxic for animals. The effect of SDHA on the liver, kidney and hematological parameters are more than those of DHA, however they are within tolerated range. Clinical and therapeutic effectiveness should determine the comparative advantages of the newly synthesized SDHA over DHA and vise versa.

show abstract

Vereinfachte Methode Der Blutzuckerbestimmung

Cited by 3 publications

References 0 publications

Biosafety of Recombinant and Wild Type Nucleopolyhedroviruses as Bioinsecticides

Biosafety of Recombinant and Wild Type Nucleopolyhedroviruses as Bioinsecticides

Changing Perspectives on Bilirubin

Effect of dihydroartemisinin and its disulphidederivative on biochemical, histological and hematological parameters in rat

Contact Info

Product

Resources

About