Vergleich von serologischen Ergänzungstests mit dem molekularbiologischen Nukleinsäuredirektnachweis in der Diagnostik der Hepatitis-C

Abstract

43 serum specimens, positive for HCV antibodies by means of an HCV-screening fest, were analysed in four hepatitis C supplementary assays: HCV EIA supplemental assay, Wellcozyme western blot, Line immuno assay and Matrix dot immuno assay. Additional a reverse transcription polymerase chain reaction (RT-PCR) was applied to hepatitis-Cvirus RNA detection in the serum samples. The supplementary tests confirmed 63%, 81% f 92%, 94% ofthe results achieved by the screening assay. Only 50% ofthe tested samples showed identical results in all four supplementary tests. In 56% ofthe sera HCVspecific RNA was detected: A correlation between the antibody reactivitypatterns in the supplementary test and the RT-PCR results could not be seen.