2014
DOI: 10.5897/ajb2013.13331
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Verification of genetic identity of introduced cacao germplasm in Ghana using single nucleotide polymorphism (SNP) markers

Abstract: Accurate identification of individual genotypes is important for cacao (Theobroma cacao L.) breeding, germplasm conservation and seed propagation. The development of single nucleotide polymorphism (SNP) markers in cacao offers an effective way to use a high-throughput genotyping system for cacao genotype verification. In the present study, high-throughput genotyping with SNP markers was used to fingerprint 160 cacao trees in the germplasm collection at the Cocoa Research Institute of Ghana (CRIG). These access… Show more

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Cited by 22 publications
(9 citation statements)
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“…This is undoubtedly less than the suspected level of mislabeling (15 to 44%) in germplasm collections worldwide 41 . Mislabeling and the verification of genetic identity from introduced as well conserved germplasm is an acute problem, as can be seen in this study and different publications over the last ten years 6,9,16,20,24,42 . Despite the fact that different applications and strategies on tackling cacao mislabeling have been provided by Turnbull et al .…”
Section: Discussionmentioning
confidence: 71%
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“…This is undoubtedly less than the suspected level of mislabeling (15 to 44%) in germplasm collections worldwide 41 . Mislabeling and the verification of genetic identity from introduced as well conserved germplasm is an acute problem, as can be seen in this study and different publications over the last ten years 6,9,16,20,24,42 . Despite the fact that different applications and strategies on tackling cacao mislabeling have been provided by Turnbull et al .…”
Section: Discussionmentioning
confidence: 71%
“…Various SNP-based genotyping methods are currently available, enabling high-throughput genotype calling without the need for electrophoresis 34 . Many SNP based genotyping methods – specific for cacao research – have been described in literature, ranging from TaqMan-based assays 12,16,23 , over Maldi TOF MS 6,24,35 , and Fluidigm SNP genotyping 15,36,37 to the competitive allele specific PCR KASPar chemistry 9 . Yet, no standard SNP genotyping method has been proposed for cacao genetic diversity studies.…”
Section: Discussionmentioning
confidence: 99%
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“…resumen, dichos resultados se traducen en la obtención de balances de rendimiento de producción, simplicidad, ahorro de costos y también; transferencia de datos moleculares de vital uso tanto para los productores como genetistas poblacionales.De alguna manera, los análisis de parentesco molecular y filogenia evolutiva de plantas han permitido a los productores de germoplasma y fitomejoradores, la generación de cultivos perennes con patrones de polinización y flujo de genes altamente rentables(Ashley 2010, Lacombe et al 2013, Takrama et al 2014, lo que lleva a comprender mejor los procesos de domesticación y selección de individuos resilientes(Motamayor et al 2002). En el caso del cacao, ciertas variedades de Trinidad y Tobago (que forman parte del germoplasma mejorado de cacao en África occidental) han mostrado altos registros de reproducción lo que se correlaciona en gran parte con la presencia de SNPs(Takrama et al 2014).Mediante análisis genéticos poblacionales que utilizan marcadores microsatelitales se ha descubierto un gran número de grupos genéticos, con una clara diferenciación entre variedades que se encuentran en la cuenca del Amazonas y variedades criollas situadas en América Central. Aunque no sabemos con total certeza cuál es el origen genético de las variedades genotipificadas en este estudio, mediante análisis de unión de secuencias nucleotídicas vecinas (NJ) (Figura Figura 7.A.…”
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