Verification of Grape Pedigree by Microsatellite Analysis

Карастан, О. М.; Мулюкина, Нина; Papina, O.

doi:10.3103/s0095452718050031

Cited by 3 publications

(3 citation statements)

References 20 publications

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“…In addition, SSRs provide unique fingerprints for cultivar identification. They are inherited by Mendelian codominant segregation, confirming their suitability for genetic resource characterization, genome mapping, assisted selection, and parentage analysis (Karastan et al, 2018;Khadivi et al, 2019;Mihaljević et al, 2020;Saifert et al, 2018;Vezzulli et al, 2019).…”

Section: Introductionmentioning

confidence: 81%

Genetic diversity, population structure and parentage analysis of Brazilian grapevine hybrids after half a century of genetic breeding

Oliveira

Niederauer

Oliveira

et al. 2023

Scientia Horticulturae

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Section: Introductionmentioning

confidence: 81%

Genetic diversity, population structure and parentage analysis of Brazilian grapevine hybrids after half a century of genetic breeding

Oliveira

Niederauer

Oliveira

et al. 2023

Scientia Horticulturae

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“…In addition, SSRs provide unique fingerprints for cultivar identification. They are inherited following Mendelian codominant segregation, confirming their suitability for genetic resource characterization, genome mapping, assisted selection, and parentage analysis (Karastan et al, 2018;Khadivi et al, 2019;Mihaljević et al, 2020;Saifert et al, 2018;Vezzulli et al, 2019).…”

Section: Introductionmentioning

confidence: 81%

Genetic diversity, population structure and parentage analysis of Brazilian grapevine hybrids after half a century of genetic breeding

Oliveira

Niederauer

Rodrigues

et al. 2022

Preprint

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In the 1940s, the Agronomic Institute (IAC) started a grapevine breeding program to develop new cultivars adapted to the tropical and subtropical regions of Brazil. More than 2,000 crosses were carried out over 50 years, using 850 varieties as parents. However, among the thousands of hybrids developed by the program, only 130 are still maintained in the IAC grapevine germplasm. Little is known about their genetic makeup and usefulness for current breeding programs. In this study, we obtained genotypes of 130 Brazilian grape hybrids at 17 polymorphic microsatellite markers to evaluate the genetic diversity and population structure of the hybrids and verified their disclosed pedigrees. The results showed that the hybrid collection is highly diverse, with an expected heterozygosity (HE) of 0.81 and an observed heterozygosity (Ho) of 0.79. A strong structure in three subgroups based mainly on the usage and combination of parental groups was revealed by STRUCTURE software and confirmed by discriminant analysis of principal components (DAPC). Through molecular profiling analysis, fifteen synonyms, one homonym and one duplicate were identified. Parentage analysis confirmed 22 full parentages, as well as 34 half-kinships. In addition, 14 pedigrees were invalidated, and eight mislabeling events were identified. No compatible parent was identified for 32.30% of the IAC hybrids, highlighting the severe genetic erosion that occurred in the IAC germplasm. The molecular characterization of the breeding hybrid bank collection contributes to our understanding the genetic basis of the varieties, guiding the efficient utilization of available genetic diversity. Together, our results could be applied to other breeding programs and assist in the selection of parents, management of the breeding collection, and conservation of grapevine genetic resources.

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“…The completion of genome sequencing of the Munake cultivar is necessary for comparative and functional genomics studies of table grapes, and the resulting genetic and genomic data will be a valuable resource for Munake cultivar breeding programs (Zhang et al, 2017;Karastan et al, 2018;Minio et al, 2019). This study describes whole-genome sequencing of the Munake cultivar and compares it with a V. vinifera L. reference genome (Jaillon et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification of Sequence Variations and SSR Marker Development in the Munake Grape Cultivar

et al. 2021

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The Munake grape cultivar produces uniquely flavored and high-quality fruits. Despite the numerous beneficial agronomic traits of Munake, there are few genetic resources available for this cultivar. To address this knowledge gap, the entire genome was sequenced using whole-genome sequencing approaches and compared with a Vitis vinifera L. reference genome. This study describes more than 3 million single nucleotide polymorphism (SNP), 300,000 insertion and deletion (InDel), 14,000 structural variation (SV), and 80,000 simple sequence repeat (SSR) markers (one SSR per 4.23 kb), as well as their primers. Among the SSRs, 44 SSR primer pairs were randomly selected and validated by polymerase chain reaction (PCR), allowing discrimination between the different Munake cultivar genotypes. The genetic data provided allow a deeper understanding of Munake cultivar genomic sequence and contribute to better knowledge of the genetic basis behind its key agronomic traits.

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Verification of Grape Pedigree by Microsatellite Analysis

Cited by 3 publications

References 20 publications

Genetic diversity, population structure and parentage analysis of Brazilian grapevine hybrids after half a century of genetic breeding

Genetic diversity, population structure and parentage analysis of Brazilian grapevine hybrids after half a century of genetic breeding

Genetic diversity, population structure and parentage analysis of Brazilian grapevine hybrids after half a century of genetic breeding

Genome-Wide Identification of Sequence Variations and SSR Marker Development in the Munake Grape Cultivar

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