Verlauf der FSH‐ und LH‐BIutpIasmakonzentrationen und der Zykluslänge beim Rind nach Applikation von boviner Follikel‐ und Zystenflüssigkeit

Abstract

Inhalt: Die vorliegenden Untersuchungen sollten A ujichluj< dariiher gehen, o b sich das Hind als Oiologisches Model1 zur llberpriifung von boviner Follikel-und -zystenfliissigkeit (BFF b z w . Z Y F ) auf Inhibinaktivitat eignet. E x p . I : J e 5 Kiihe wurden a m 16. Zyklustag 3 Ma1 im Abstand von 1 2 Stunden m i t j e 20 ml BFF oder phys. NaCl-Losung i.v. und 1 0 pg G n R H i.m. zum Zeitpunkt der letzt e n Injektion behandelt. Die GnKII-induzierten FSH-und LEI-Gipfel wurden durch B FF n ic h t signifika nt b eein f lufi 1. E x p . 2: J e 5 Kiihe wurden a m 16. Zyklustag 3 Ma1 im Abstand uon 1 2 Stunden m i t j e 2 0 ml BFF brw. Z Y F b z w . phys. NaCl-Losung i.v. behandett. I m Gegensatz zu Z Y F fiihrte BFF schon 1 2 Stunden nach der ersten Verabreichung zu einer selektiven H e mm u n g der FSH-Konzentrationen. Die FSH-Konzentrationen blieben nach der BFF-Behandlung iiher 2 4 Stunden auf 60-75% der Ausgangswerte erniedrigt. Die Rrunstintervalle waren bei d e n RFF-und Z YF-behandelten Kuhen gegeniiber den Kontrollticren u m 5.5 b z w . 3 Tage verlangert. Das Kind eignet sich zur Untersuchung FSH-hemmender Substanzen auf ihre Inhibinaktivitat. (BFF and Z Y F resp.). E x p . 1 : 5 cows each were treated on day 16 and 1 7 o j t h e cycle 3 times at intervals of 1 2 hours with either 20 m l B F F or phys. NaCl-solution i.v. and 10 pg G n R H i.m. at the time of the last injection. The GnKII-induced FSEI-and LH-peaks were not significantly suppressed b y BFF. E x p . 2: 5 cows each were treated o n day 16 and 1 7 o f t h e cycle 3 times at intervals o f 12 hours with either 2 0 ml Contents: "Influence of fluid from ovarian follicles and cysts on FSH and LH blood plasma concentrations and on the length of the estrous cycle in cattle". The present investigations should enlighten t h e question, if the bovine is suitable as an in vivo bioassay model t o examine the inhibin-like activity o f bovine follicular-and bovine cystic fluid . T h e FSH-concentrations decreased 12 hours following the B F F application and remained depressed over 24 hours at 60-75% of t h e values before treatment. T h e LH-concentrations were n o t significantly influenced. T h e injection of Z Y F did not change the overall FSH-concentrations. The bovine is suitable t o examine t h e inhibin-like activity of bovine folliculafluid.Einleitung Seit wenigen Jahren ist bekannt, dal3 Follikelfliissigkeit verschiedener Spezies einen Faktor enthalt, der die FSH-Sekretion selektiv unterdruckt. Diese FSH-hemmende Ak-U.S.

Plasma concentrations of gonadotrophins, preovulatory follicular development and luteal function associated with bovine follicular fluid-induced delay of oestrus in heifers

“…2 are consistent with other reports in cattle that injections of follicular fluid selectively suppress plasma concentrations of FSH (Ireland et al, 1983;Braun et al, 1983;. In contrast, reported that plasma concentrations of FSH were not decreased significantly during treatment of heifers with injections of follicular fluid on Days 1 through 5 of the oestrous cycle.…”

“…Injections of bovine follicular fluid during the follicular phase of the oestrous cycle in heifers and ewes lengthened the interval from luteolysis to oestrus (Miller, Critser, Rowe & Ginther, 1979) and subsequent studies with ewes showed that plasma concentrations of FSH decreased during treatment (Miller, Critser & Ginther, 1982;McNeilly, 1984). Injections of bovine follicular fluid to ovariectomized heifers, prepubertal heifers and cyclic heifers during the late luteal phase of the oestrous cycle decreased plasma concentrations of FSH, but did not alter plasma concentrations of LH (Ireland, Curato & Wilson, 1983;Braun, Schams, Buck & Leidl, 1983;Johnson, Smith & Elmore, 1985). The aim of the present study was to determine whether treatment of heifers with bovine follicular fluid during the follicular phase of the oestrous cycle would selectively suppress plasma concentrations of FSH and, if so, whether this would alter preovulatory follicular development and/or subsequent luteal function.…”