Vernonia amygdalina extract induces apoptosis and inhibits epithelial-mesenchymal transition in Hep 3B cells through the inhibition of PI3k/Akt signaling pathway

Lai, Chen-Chu; Zhou, Xinran; Wang, Hao‐Kuang; Lin, Yingchao; Lin, Hui-Yi; Way, Tzong‐Der; Liu, Bing-Lan

doi:10.6703/ijase.202203_19(1).002

Int. J. Appl. Sci. Eng.

2022

DOI: 10.6703/ijase.202203_19(1).002

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Vernonia amygdalina extract induces apoptosis and inhibits epithelial-mesenchymal transition in Hep 3B cells through the inhibition of PI3k/Akt signaling pathway

Chen-Chu Lai

Xinran Zhou

Hao‐Kuang Wang

et al.

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“…Usmani et al, 2019, Ghareeb et al, 2019, Ige et al, 2017 Zingiber officinale, Curcuma Longa, Coconut Oil, Vernonia amygdalinaLai et al, 2021, Ezzat et al, 2020, Ige et al, 2017 Curcuma longa, Glycyrrhiza glabra, Moringa oleiferaAshraf et al, 2018, Hassan et al, 2015, Sarfo-Antwi et al, 2018 …”

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Hepatoprotective Activity of Plant Extracts in Non-Human Models: The Toxicants Solvents and Diseased Models-A Review

Dongsogo,

Larbie,

Idrissu

et al. 2023

Am. J. Multidis. Res. Innov.

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The search for an ideal hepatic regenerative agent has led to the modelling of several forms of liver disorders. This involves the usage of various substances in in-vitro and in-vivo systems that are injurious to the human liver in in-vitro and in-vivo systems and then challenged with plants parts extracted with organic and inorganic solvents. Reported view works on hepatotoxicity have been focused on the plant species, this review therefore focused on the toxicants, the solvents for extraction and models used in inducing the hepatotoxicity. Google search, Elsevier and PubMed databases were searched for primary articles from 2005-2022 on hepatotoxicity. In all, 206 articles were retrieved, of which 46 were rejected and 165 were included for the analysis. Authors reported 55 toxicants used in inducing 10 in-vitro and in-vitro models and challenged with 94 different medicinal plants species extracted using 11 different organic and inorganic solvents. Comparatively, tetrachloromethane (CCL4) was the most frequent toxicant 55 of 94 (58.5%) reported plant species while ethanol 25 (45.5%) was the most frequently used solvent for extracting plant phytoconstituents. For the models used in inducing hepatotoxicity, Wistar albino rats 46 of the 55 (83.6%) reported toxicants was the most predominant. The liver is prone to many available toxicants however, phytohepatoregeneration is possible with many medicinal plants which can easily be extracted with common solvents.

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mentioning

confidence: 99%

Hepatoprotective Activity of Plant Extracts in Non-Human Models: The Toxicants Solvents and Diseased Models-A Review

Dongsogo,

Larbie,

Idrissu

et al. 2023

Am. J. Multidis. Res. Innov.

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Vernonia amygdalina Leaf Extract Induces Apoptosis in HeLa Cells: A Metabolomics and Proteomics Study

Samutrtai,

Yingchutrakul,

Faikhruea

et al. 2024

Pharmaceuticals

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Medicinal plants produce various bioactive molecules with potential anti-cancer properties with favorable safety profiles. We aimed to investigate the comprehensive composition of Vernonia amygdalina leaf extract and its cytotoxic effects via apoptosis in HeLa cells. The metabolomics approach using LC-MS/MS was conducted to gather the metabolite profile of the extract. Proteomics was performed to understand the comprehensive mechanistic pathways of action. The apoptosis was visualized by cellular staining and the apoptotic proteins were evaluated. V. amygdalina leaf extract exhibited dose-dependent cytotoxic effects on both HeLa and Vero cells after 24 h of exposure in the MTT assay with the IC50 values of 0.767 ± 0.0334 and 4.043 ± 0.469 µg mL−1, respectively, which demonstrated a higher concentration required for Vero cell cytotoxicity. The metabolomic profile of 112 known metabolites specified that the majority of them were alkaloids, phenolic compounds, and steroids. Among these metabolites, deacetylvindoline and licochalcone B were suggested to implicate cytotoxicity. The cytotoxic pathways involved the response to stress and cell death which was similar to doxorubicin. The upstream regulatory proteins, phosphatase and tensin homolog deleted on chromosome ten (PTEN) and X-box binding protein 1 (XBP1), were significantly altered, supporting the regulation of apoptosis and cell death. The levels of apoptotic proteins, c-Jun N-terminal kinases (JNK), p53, and caspase-9 were significantly increased. The novel insights gained from the metabolomic profiling and proteomic pathway analysis of V. amygdalina leaf extract have identified crucial components related to apoptosis induction, highlighting its potential to develop future chemotherapy.

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Vernonia amygdalina extract induces apoptosis and inhibits epithelial-mesenchymal transition in Hep 3B cells through the inhibition of PI3k/Akt signaling pathway

Cited by 2 publications

References 0 publications

Hepatoprotective Activity of Plant Extracts in Non-Human Models: The Toxicants Solvents and Diseased Models-A Review

Hepatoprotective Activity of Plant Extracts in Non-Human Models: The Toxicants Solvents and Diseased Models-A Review

Vernonia amygdalina Leaf Extract Induces Apoptosis in HeLa Cells: A Metabolomics and Proteomics Study

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