Versatile bio-ink for covalent immobilization of chimeric avidin on sol–gel substrates

Fabrication of Carboxylated Silicon Nitride Sensor Chips for Detection of Antigen–Antibody Reaction Using Microfluidic Reflectometric Interference Spectroscopy

“…The silane coupling reaction is one of the most commonly used coupling techniques for surface modification of biosensing chips. Among silane coupling agents, in particular, 3-aminopropyltriethoxysilane − and 3-glycidoxypropyltrimethoxysilane , are often chosen to immobilize biomolecules on an oxide (or oxide-like) surface in conjunction with the use of heterobifunctional linkers such as N -succinimidyl-3-(2-pyridyldithio) propionate, N -(6-maleimidocaproyloxy) succinimide, and biotin N -hydroxysuccinimide ester.…”

Printing technologies for biomolecule and cell-based applications

“…In order to achieve a good ink transfer, a relative high print pressure (1-5 MPa) is used and the ink should have a rather low viscosity of 50-200 mPa s. However, inks with viscosities down to 1-10 mPa have been successfully gravure printed (Hambsch et al, 2010;Voigt et al, 2010). Heikkinen et al have shown that gravure printing can be used for a high-thoughput deposition of biomolecules (Heikkinen et al, 2011). Chimeric avidin protein patterns were gravure-printed on Fig.…”

Biomolecule immobilization techniques for bioactive paper fabrication

“…They also described laccase encapsulated in cross-linked polyethyleneimine and deposited on paper. Heikkinen et al [23] prepared bioink from the heterobifunctional crosslinker 6-maleimidohexanoic acid N-hydroxysuccinimide, which was first reacted with 3-aminopropyltriethoxysilane to form the silane linker 6-maleimide-N-(3-(triethoxydilyl) propyl)hexanamide. Carboxy-terminal cysteine genetically engineered to chimeric avidin was reacted with the maleimide group of the silane linker in methanol/phosphate-buffered saline solution to form a suspension, which was printed on sol-gel-modified poly(methyl methacrylate) film.…”