Versatile Cloning Vectors Constructed with Genes Indigenous to a Glutamic Acid-Producer,<i>Brevibacterium lactofermentum</i>

Morinaga, Yasushi; Miwa, Kiyoshi; Nakamori, Shigeru; Sano, Konosuke

doi:10.1080/00021369.1986.10867792

“…Selective markers were needed since these plasmids are cryptic. Several common antibiotic resistance markers were tried but owing to the high intrinsic threshold level of resistance of corynebacteria, only a handful of these resistance genes are now in use, including the kanamycin resistance (Km r ) gene of Tn 5 and the trimethoprim resistance gene of B. lactofermentum [23, 42]. In addition, the streptomycin and spectinomycin resistance genes also can be used in these bacteria.…”

Section: Plasmids As Cloning Vectorsmentioning

confidence: 99%

Plasmids of corynebacteria

Deb

¹

,

Nath

²

1999

FEMS Microbiology Letters

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Corynebacteria are pleomorphic, asporogenous, Gram-positive bacteria. Included in this group are nonpathogenic soil corynebacteria, which are widely used for the industrial production of amino acids and detergents, and in biotransformation of steroids. Other members of this group are plant and animal pathogens. This review summarizes the current information available about the plasmids of corynebacteria. The emphasis is mainly on the small plasmids, which have been used for construction of vectors for expression of genes in these bacteria. Moreover, considerable information is now available on their nucleotide sequence, gene organization and modes of replication, which would make it possible to further manipulate these plasmids. Other plasmid properties, such as incompatibility and host range, are also discussed. Finally, use of these plasmids as cloning vectors for the expression of heterologous proteins using corynebacteria as hosts is also summarized to highlight the potential of these bacteria as hosts for recombinant DNA.

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“…Selective markers were needed since these plasmids are cryptic. Several common antibiotic resistance markers were tried but owing to the high intrinsic threshold level of resistance of corynebacteria, only a handful of these resistance genes are now in use, including the kanamycin resistance (Km r ) gene of Tn5 and the trimethoprim resistance gene of B. lactofermentum [23,42]. In addition, the streptomycin and spectinomycin resistance genes also can be used in these bacteria.…”

Section: Plasmids As Cloning Vectorsmentioning

confidence: 99%

Plasmids of corynebacteria

Deb

¹

1999

FEMS Microbiology Letters

View full text Add to dashboard Cite

Corynebacteria are pleomorphic, asporogenous, Gram-positive bacteria. Included in this group are nonpathogenic soil corynebacteria, which are widely used for the industrial production of amino acids and detergents, and in biotransformation of steroids. Other members of this group are plant and animal pathogens. This review summarizes the current information available about the plasmids of corynebacteria. The emphasis is mainly on the small plasmids, which have been used for construction of vectors for expression of genes in these bacteria. Moreover, considerable information is now available on their nucleotide sequence, gene organization and modes of replication, which would make it possible to further manipulate these plasmids. Other plasmid properties, such as incompatibility and host range, are also discussed. Finally, use of these plasmids as cloning vectors for the expression of heterologous proteins using corynebacteria as hosts is also summarized to highlight the potential of these bacteria as hosts for recombinant DNA. z

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Molecular breeding of a Brevibacterium lactofermentum l-phenylalanine producer using a cloned prephenate dehydratase gene

Ito

¹

,

Sato

²

,

Matsui

³

et al. 1990

Appl Microbiol Biotechnol

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The prephenate dehydratase gene was cloned from a mutant of Brevibacterium lactofermenturn, AJl1957 that produced enzyme free from feedback inhibition. The recombinant plasmids pPHI 1 and pPH14 complemented a phenylalanine auxotroph of B. lactofermenturn, A-15, provided the transformant with the desensitized enzyme and caused an increased level of the enzyme compared to that of a wild strain. Plasmid pPH14 was introduced into L-phenylalanine producers genetically induced from Bo lactofermentum; MF358 and FP-1 excreting L-tyrosine and anthranilate, respectively, as by-products. Both transformants predominantly accumulated L-phenylalanine at the expense of by-product formation. Co-existence of pPH14 and pTAR16, a recombinant plasmid expressing desensitized 3-deoxy-D-arabino-hepturosonate-7-phosphate synthase had a marked effect on further improvement in L-phenylalanine productivity, accompanied by an increase in the corresponding enzyme activity. The parent, MF358, accumulating 5.5g/1 L-phenylalanine, 6.8 g/1 L-tyrosine and 0.3 g/1 anthranilate turned into a potent L-phenylalanine producer producing 18.2 g/1 Lphenylalanine and 1.0 g/1 L-tyrosine by-product.

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Versatile Cloning Vectors Constructed with Genes Indigenous to a Glutamic Acid-Producer,Brevibacterium lactofermentum

Cited by 5 publications

References 4 publications

Plasmids of corynebacteria

Plasmids of corynebacteria

Plasmids of corynebacteria

Molecular breeding of a Brevibacterium lactofermentum l-phenylalanine producer using a cloned prephenate dehydratase gene

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