Versatile fabrication of vascularizable scaffolds for large tissue engineering in bioreactor

Tocchio, Alessandro; Tamplenizza, Margherita; Martello, Federico; Gerges, Irini; Rossi, Eleonora; Argentiere, Simona; Rodighiero, Simona; Zhao, Weiwei; Milani, P.; Lenardi, Cristina

doi:10.1016/j.biomaterials.2014.12.031

Cited by 117 publications

(89 citation statements)

References 47 publications

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“…Multiple methods have been reported to create hollow channels in polymeric biomaterial scaffolds, including the use of multi-material 3D fiber deposition [26,27], electrospinning [28], the casting of scaffold material around sacrificial materials [29,30], laser drilling [31], and the use of silicon molds [32,33] to replicate patterns. Even though these approaches have been successful in generating well-organized endothelialized vascular networks, these structures are generally bordered by a dense, impenetrable layer of biomaterial, limiting further vascular remodeling and nutrient transport.…”

Section: Patterning Of Endothelial Cells In Engineered Tissuesmentioning

confidence: 99%

Vascularization and Angiogenesis in Tissue Engineering: Beyond Creating Static Networks

Rouwkema

Khademhosseini

2016

Trends in Biotechnology

538

453

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Section: Patterning Of Endothelial Cells In Engineered Tissuesmentioning

confidence: 99%

Vascularization and Angiogenesis in Tissue Engineering: Beyond Creating Static Networks

Rouwkema

Khademhosseini

2016

Trends in Biotechnology

538

453

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“…In addition to the biochemical signalling provided to cells by ECM proteins, stiffness, topography and geometry of the extracellular space also modulate cell behaviour and phenotype123. Recently, three-dimensional geometry has been reported as an important feature of synthetic scaffolds favoring cell stemness, maintenance and differentiation, nerve regeneration and vascularization11121314. Further evidence of the role of matrix geometry is the fact that a depletion of all integrins does not impede the movement of dendritic cells in 3D matrices15, whereas matrix geometry and the resulting cell confinement were the main parameters regulating cell phenotype and cell behavior161718.…”

mentioning

confidence: 99%

Linearized texture of three-dimensional extracellular matrix is mandatory for bladder cancer cell invasion

Alfano

Nebuloni

Allevi

et al. 2016

Sci Rep

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In the fields of biomaterials and tissue engineering simulating the native microenvironment is of utmost importance. As a major component of the microenvironment, the extracellular matrix (ECM) contributes to tissue homeostasis, whereas modifications of native features are associated with pathological conditions. Furthermore, three-dimensional (3D) geometry is an important feature of synthetic scaffolds favoring cell stemness, maintenance and differentiation. We analyzed the 3D structure, geometrical measurements and anisotropy of the ECM isolated from (i) human bladder mucosa (basal lamina and lamina propria) and muscularis propria; and, (ii) bladder carcinoma (BC). Next, binding and invasion of bladder metastatic cell line was observed on synthetic scaffold recapitulating anisotropy of tumoral ECM, but not on scaffold with disorganized texture typical of non-neoplastic lamina propria. This study provided information regarding the ultrastructure and geometry of healthy human bladder and BC ECMs. Likewise, using synthetic scaffolds we identified linearization of the texture as a mandatory feature for BC cell invasion. Integrating microstructure and geometry with biochemical and mechanical factors could support the development of an innovative synthetic bladder substitute or a tumoral scaffold predictive of chemotherapy outcomes.

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“…Various attempts have been made to generate a functional vasculature in recent years, including biochemical modification [18][19][20][21], exogenous cells [10,[22][23][24], and microengineering technology [25][26][27][28][29][30]. Most of these attempts, however, have only focused on applying exogenous vascular cells and angiogenic factors.…”

Section: Introductionmentioning

confidence: 99%

Ectopic tissue engineered ligament with silk collagen scaffold for ACL regeneration: A preliminary study

Ran

et al. 2017

Acta Biomaterialia

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a b s t r a c tAnterior cruciate ligament (ACL) reconstruction remains a formidable clinical challenge because of the lack of vascularization and adequate cell numbers in the joint cavity. In this study, we developed a novel strategy to mimic the early stage of repair in vivo, which recapitulated extra-articular inflammatory response to facilitate the early ingrowth of blood vessels and cells. A vascularized ectopic tissue engineered ligament (ETEL) with silk collagen scaffold was developed and then transferred to reconstruct the ACL in rabbits without interruption of perfusion. At 2 weeks after ACL reconstruction, more wellperfused cells and vessels were found in the regenerated ACL with ETEL, which decreased dramatically at the 4 and 12 week time points with collagen deposition and maturation. ACL treated with ETEL exhibited more mature ligament structure and enhanced ligament-bone healing post-reconstructive surgery at 4 and 12 weeks, as compared with the control group. In addition, the ETEL group was demonstrated to have higher modulus and stiffness than the control group significantly at 12 weeks post-reconstructive surgery. In conclusion, our results demonstrated that the ETEL can provide sufficient vascularity and cellularity during the early stages of healing, and subsequently promote ACL regeneration and ligament-bone healing, suggesting its clinic use as a promising therapeutic modality. Statement of SignificanceEarly inflammatory cell infiltration, tissue and vessels ingrowth were significantly higher in the extraarticular implanted scaffolds than theses in the joint cavity. By mimicking the early stages of wound repair, which provided extra-articular inflammatory stimulation to facilitate the early ingrowth of blood vessels and cells, a vascularized ectopic tissue engineered ligament (ETEL) with silk collagen scaffold was constructed by subcutaneous implantation for 2 weeks. The fully vascularized TE ligament was then transferred to rebuild ACL without blood perfusion interruption, and was demonstrated to exhibit improved ACL regeneration, bone tunnel healing and mechanical properties.

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Versatile fabrication of vascularizable scaffolds for large tissue engineering in bioreactor

Cited by 117 publications

References 47 publications

Vascularization and Angiogenesis in Tissue Engineering: Beyond Creating Static Networks

Vascularization and Angiogenesis in Tissue Engineering: Beyond Creating Static Networks

Linearized texture of three-dimensional extracellular matrix is mandatory for bladder cancer cell invasion

Ectopic tissue engineered ligament with silk collagen scaffold for ACL regeneration: A preliminary study

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