Versatile fiber-reinforced hydrogels to mimic the microstructure and mechanics of human vocal-fold upper layers

Ferri-Angulo, Daniel; Yousefi-Mashouf, Hamid; Michel, Margot; McLeer, Anne; Orgéas, Laurent; Bailly, Lucie; Sohier, Jérôme

doi:10.1016/j.actbio.2023.09.035

Cited by 5 publications

(5 citation statements)

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“…These non-physiological tendencies can be explained by several discrepancies that remain between the histo-mechanical characteristics of a biological larynx and our current in vitro idealization: firstly, the longitudinal tensile response of all the materials studied in this work is still quite far from that of native vocal-fold tissues, even for the optimized hydrogel, due to its isotropy. In particular, the non-linear strain-hardening of tangent moduli observed on excised human 34 , 59 , 114 or animal vocal folds 52 , 53 , which is linked to the progressive recruitment, deployment and reorientation of collagen fibres towards the load direction 34 , 35 , 49 , 115 , is not yet reproduced 26 , 48 ; then, the laryngeal envelope and induced boundary conditions are probably still too soft to mimic the stiffness of native cartilages; the “active” hardening of the vocalis during its contraction in vivo is also left out of the current replica. Finally, the sound quality produced by the four candidates is affected.…”

Section: Resultsmentioning

confidence: 99%

“…One key limitation of the study is the nature of our current synthetic vocal folds made of a simplified, single-layered structure filled with homogeneous materials, which are still unable to reproduce the highly non-linear mechanical behavior of native vocal folds under finite-strains tension. The embedding of fibrous reinforcement with suitable microstructure gradients in the upper layers 49 should allow to approach the J-shaped anisotropic target response in tension, and extend the phonation capabilities of artificial replicas to bring them closer to physiological ranges. Also, the use of more rigid lateral boundaries on the laryngeal envelope, such as a stiffer cartilaginous glottal stage casing, would enable more physiological geometry and vibratory patterns to be achieved.…”

Section: Discussionmentioning

confidence: 99%

“…Today, the development of improved vocal-fold replicas and enriched experiments is still necessary to better understand the complexity of the multiphysical couplings involved, on the one hand, and to evaluate and dialogue with the various theories and numerical models mentioned above, on the other hand. Thus, in recent years, while improving current manufacturing procedures 45 , 46 , the search for optimal materials 47 – 49 , multi-scale structures 49 and mechanical control 50 , 51 for increasingly “bio-/phono-mimetic” vocal-fold replicas is the subject of active investigation. However: Even though vocal-fold stretching is a major aspect of phonation biomechanical control 3 , 32 – 35 , 39 , 52 , 53 , the number of in vitro studies involving experimental models of vocal folds able to measure and control the laryngeal longitudinal pre-strain occurring before any phonatory event is very limited.…”

Section: Introductionmentioning

confidence: 99%

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Flow-induced oscillations of vocal-fold replicas with tuned extensibility and material properties

Luizard,

Bailly,

Yousefi-Mashouf

et al. 2023

Sci Rep

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Human vocal folds are highly deformable non-linear oscillators. During phonation, they stretch up to 50% under the complex action of laryngeal muscles. Exploring the fluid/structure/acoustic interactions on a human-scale replica to study the role of the laryngeal muscles remains a challenge. For that purpose, we designed a novel in vitro testbed to control vocal-folds pre-phonatory deformation. The testbed was used to study the vibration and the sound production of vocal-fold replicas made of (i) silicone elastomers commonly used in voice research and (ii) a gelatin-based hydrogel we recently optimized to approximate the mechanics of vocal folds during finite strains under tension, compression and shear loadings. The geometrical and mechanical parameters measured during the experiments emphasized the effect of the vocal-fold material and pre-stretch on the vibration patterns and sounds. In particular, increasing the material stiffness increases glottal flow resistance, subglottal pressure required to sustain oscillations and vibratory fundamental frequency. In addition, although the hydrogel vocal folds only oscillate at low frequencies (close to 60 Hz), the subglottal pressure they require for that purpose is realistic (within the range 0.5–2 kPa), as well as their glottal opening and contact during a vibration cycle. The results also evidence the effect of adhesion forces on vibration and sound production.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Flow-induced oscillations of vocal-fold replicas with tuned extensibility and material properties

Luizard,

Bailly,

Yousefi-Mashouf

et al. 2023

Sci Rep

Self Cite

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“…One approach to improve the stiffness, strength, and toughness of hydrogels is to introduce fibrous reinforcement, for example with electrospun polymer mats or glass fibers 7–11 . Hydrogel‐based systems for use in biomedical applications have been investigated that utilize this strategy, such as a polycaprolactone (PCL) fiber‐reinforced polyethylene glycol (PEG) mimic of human vocal‐fold tissue 12 . Mechanical models to describe the behavior of such composite materials are well developed for more conventional composites utilizing semicrystalline polymeric matrices, such as epoxy resins, and can be used to design materials with specific mechanical properties 13–18 .…”

Section: Introductionmentioning

confidence: 99%

Predicting the tensile and compressive modulus of electrospun fiber mat‐reinforced hydrogels using the Halpin–Tsai equations

Beckett,

Lewis,

Tonge

et al. 2024

J of Applied Polymer Sci

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The reinforcement of mechanically‐weak hydrogels to yield composites with increased stiffness, strength, or toughness is a well‐established approach. In particular, introducing electrospun nanofibers into hydrogels is a common strategy for biomedical applications, as the resulting hierarchical structure mimics biology and allows for control over fiber diameter and alignment and tuning of mechanical properties. However, further study of the link between the constituent materials and the mechanical properties of the composite is uncommon. One potential model to understand the mechanical properties of fiber‐reinforced hydrogels involves the Halpin–Tsai equations, which relate the modulus values of the fibers and hydrogel matrix and the fiber volume fraction, to the modulus of the composite. To assess the application of this model to fiber‐reinforced hydrogels, predicted values were compared with experimental values from mechanical testing of a poly(ethylene glycol) (PEG) matrix reinforced with an electrospun polycaprolactone (PCL) fiber mat. Although the equations described these systems well in tension, providing a facile approach to identify a fiber volume fraction that will achieve a desired modulus, the Halpin–Tsai approach was less successful under compression. This study motivates additional investigation of the role of structural features of hydrogel composites in determining mechanical properties to enable design of materials for specific applications.

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“…In recent years, with the development of chemical synthesis and characterization technology, scientists have synthesized a series of dendrimers, such as polyamidoamine (PAMAM) dendrimers, polypropyleneimine dendrimers, peptide dendrimers, glycan dendrimers, etc. [ 13 , 14 , 15 , 16 , 17 , 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Recent Research Progress on Polyamidoamine-Engineered Hydrogels for Biomedical Applications

Liu,

Li,

Yang

et al. 2024

Biomolecules

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Hydrogels are three-dimensional crosslinked functional materials with water-absorbing and swelling properties. Many hydrogels can store a variety of small functional molecules to structurally and functionally mimic the natural extracellular matrix; hence, they have been extensively studied for biomedical applications. Polyamidoamine (PAMAM) dendrimers have an ethylenediamine core and a large number of peripheral amino groups, which can be used to engineer various polymer hydrogels. In this review, an update on the progress of using PAMAM dendrimers for multifunctional hydrogel design was given. The synthesis of these hydrogels, which includes click chemistry reactions, aza-Michael addition, Schiff base reactions, amidation reactions, enzymatic reactions, and radical polymerization, together with research progress in terms of their application in the fields of drug delivery, tissue engineering, drug-free tumor therapy, and other related fields, was discussed in detail. Furthermore, the biomedical applications of PAMAM-engineered nano-hydrogels, which combine the advantages of dendrimers, hydrogels, and nanoparticles, were also summarized. This review will help researchers to design and develop more functional hydrogel materials based on PAMAM dendrimers.

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Versatile fiber-reinforced hydrogels to mimic the microstructure and mechanics of human vocal-fold upper layers

Cited by 5 publications

References 76 publications

Flow-induced oscillations of vocal-fold replicas with tuned extensibility and material properties

Flow-induced oscillations of vocal-fold replicas with tuned extensibility and material properties

Predicting the tensile and compressive modulus of electrospun fiber mat‐reinforced hydrogels using the Halpin–Tsai equations

Recent Research Progress on Polyamidoamine-Engineered Hydrogels for Biomedical Applications

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