Versatile microfluidic complement fixation test for disease biomarker detection

Li, Man; Shi, Zhuanzhuan; Fang, Can; Gao, AnXiu; Li, Chang Ming; Yu, Ling

doi:10.1016/j.aca.2016.02.026

Cited by 5 publications

(4 citation statements)

References 20 publications

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“…One recent example used an on-chip complement fixation test for the detection of carcinoembryonic antigen and recombinant avian influenza A virus using a PDMS-glass microchip [22]. Fluidic-based and agar gel-based complement fixation tests were developed to indicate the presence of a specific antibody or antigen.…”

Section: On-chip Sample Preparation Methodsmentioning

confidence: 99%

Recent advances in microfluidic sample preparation and separation techniques for molecular biomarker analysis: A critical review

Sonker

Sahore

Woolley

2017

Analytica Chimica Acta

142

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Microfluidics is a vibrant and expanding field that has the potential for solving many analytical challenges. Microfluidics show promise to provide rapid, inexpensive, efficient, and portable diagnostic solutions that can be used in resource-limited settings. Researchers have recently reported various microfluidic platforms for biomarker analysis applications. Sample preparation processes like purification, preconcentration and labeling have been characterized on-chip. Additionally, improvements in microfluidic separation techniques have been reported for cellular and molecular biomarkers. This review critically evaluates microfluidic sample preparation platforms and separation methods for biomarker analysis reported in the last two years. Key advances in device operation and ability to process different sample matrices in a variety of device materials are highlighted. Finally, current needs and potential future directions for microfluidic device development to realize its full diagnostic potential are discussed.

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Section: On-chip Sample Preparation Methodsmentioning

confidence: 99%

Recent advances in microfluidic sample preparation and separation techniques for molecular biomarker analysis: A critical review

Sonker

Sahore

Woolley

2017

Analytica Chimica Acta

142

View full text Add to dashboard Cite

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“…Moreover, POC tests can be coupled to smartphones and analyze testing results, giving quantitative information and strengthening its potential as a POC. However, it is necessary to standardize the conditions of use to ensure that the result can be read without interference [ 21 , 37 ].…”

Section: Main Components Of Paper-based Point-of-care Platformsmentioning

confidence: 99%

Current Challenges and Future Trends of Enzymatic Paper-Based Point-of-Care Testing for Diabetes Mellitus Type 2

2021

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A point-of-care (POC) can be defined as an in vitro diagnostic test that can provide results within minutes. It has gained enormous attention as a promising tool for biomarkers detection and diagnosis, as well as for screening of chronic noncommunicable diseases such as diabetes mellitus. Diabetes mellitus type 2 is one of the metabolic disorders that has grown exponentially in recent years, becoming one of the greatest challenges to health systems. Early detection and accurate diagnosis of this disorder are essential to provide adequate treatments. However, efforts to reduce incidence should remain not only in these stages but in developing continuous monitoring strategies. Diabetes-monitoring tools must be accessible and affordable; thus, POC platforms are attractive, especially paper-based ones. Paper-based POCs are simple and portable, can use different matrixes, do not require highly trained staff, and are less expensive than other platforms. These advantages enhance the viability of its application in low-income countries and hard-to-reach zones. This review aims to present a critical summary of the main components required to create a sensitive and affordable enzymatic paper-based POC, as well as an oriented analysis to highlight the main limitations and challenges of current POC devices for diabetes type 2 monitoring and future research opportunities in the field.

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“…In shell viral culture, cells are grown in 1 dram shell vials and stained with specific monoclonal antibodies for influenza detection. Serological tests include hemagglutination inhibition (HAI), [135][136][137] virus neutralization (VN), 136,138 complement fixation, 139,140 single radial hemolysis tests (SHR), 136,137,141 and enzyme-linked immunosorbent assay (ELISA). 128,136,[142][143][144] ELISA, one of the most commonly performed laboratory assays, uses mono-and polyclonal antibodies against nucleoproteins on the influenza virus.…”

Section: Current Diagnosis Of H7n9 Influenza Virus Infectionmentioning

confidence: 99%

Virus-induced pathogenesis, vaccine development, and diagnosis of novel H7N9 avian influenza A virus in humans: a systemic literature review

Mrc

et al. 2019

J Int Med Res

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H7N9 avian influenza virus (AIV) caused human infections in 2013 in China. Phylogenetic analyses indicate that H7N9 AIV is a novel reassortant strain with pandemic potential. We conducted a systemic review regarding virus-induced pathogenesis, vaccine development, and diagnosis of H7N9 AIV infection in humans. We followed PRISMA guidelines and searched PubMed, Web of Science, and Google Scholar to identify relevant articles published between January 2013 and December 2018. Pathogenesis data indicated that H7N9 AIV belongs to low pathogenic avian influenza, which is mostly asymptomatic in avian species; however, H7N9 induces high mortality in humans. Sporadic human infections have recently been reported, caused by highly pathogenic avian influenza viruses detected in poultry. H7N9 AIVs resistant to adamantine and oseltamivir cause severe human infection by rapidly inducing progressive acute community-acquired pneumonia, multiorgan dysfunction, and cytokine dysregulation; however, mechanisms via which the virus induces severe

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Versatile microfluidic complement fixation test for disease biomarker detection

Cited by 5 publications

References 20 publications

Recent advances in microfluidic sample preparation and separation techniques for molecular biomarker analysis: A critical review

Recent advances in microfluidic sample preparation and separation techniques for molecular biomarker analysis: A critical review

Current Challenges and Future Trends of Enzymatic Paper-Based Point-of-Care Testing for Diabetes Mellitus Type 2

Virus-induced pathogenesis, vaccine development, and diagnosis of novel H7N9 avian influenza A virus in humans: a systemic literature review

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